Merck
Todas as fotos(5)

Documentos

C7661

Sigma-Aldrich

Colágeno

Bornstein and Traub Type I, powder, BioReagent, suitable for cell culture

Faça loginto View Organizational & Contract Pricing

Número CAS:
Número CE:
Número MDL:
NACRES:
NA.75

fonte biológica

rat tail

linha de produto

BioReagent

forma

powder

peso molecular

120—160 kDa

embalagem

glass bottle of 5 mg

technique(s)

cell culture | mammalian: suitable

cobertura de superfície

6‑10 μg/cm2

solubilidade

soluble (Product is clear to hazy colorless solution with a few insolubles at 1 mg/ml in water with 2 μl acetic acid (or 0.1 N acetic acid). The insolubles can be removed by settling or centrifugation.)

nº de adesão NCBI

nº de adesão UniProt

Especificidade de ligação

Peptide Source: Fibrinogen

Peptide Source: Laminin

temperatura de armazenamento

2-8°C

Gene Information

Procurando produtos similares? Visit Guia de comparação de produtos

Categorias relacionadas

Comparar Itens Semelhantes

Ver Comparação Total

Mostrar diferenças

1 of 4

Este Item
C386711179179001C9791
Collagen from rat tail Bornstein and Traub Type I, powder, BioReagent, suitable for cell culture

Sigma-Aldrich

C7661

Collagen from rat tail

Sigma-Aldrich

Sigma-Aldrich

C3867

Collagen, Type I solution from rat tail

Collagen from rat tail tendon

Roche

11179179001

Collagen

Collagen from calf skin Bornstein and Traub Type I, solid, BioReagent, suitable for cell culture

Sigma-Aldrich

C9791

Collagen from calf skin

form

powder

form

liquid

form

lyophilized (clear, colorless solution after reconstitution)

form

solid

packaging

glass bottle of 5 mg

packaging

pkg of 1 vial

packaging

pkg of 30 mg

packaging

poly bottle of 10 mg, poly bottle of 100 mg, poly bottle of 250 mg, poly bottle of 50 mg

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

surface coverage

6‑10 μg/cm2

surface coverage

6‑10 μg/cm2

surface coverage

-

surface coverage

6‑10 μg/cm2

solubility

soluble (Product is clear to hazy colorless solution with a few insolubles at 1 mg/ml in water with 2 μl acetic acid (or 0.1 N acetic acid). The insolubles can be removed by settling or centrifugation.)

solubility

-

solubility

-

solubility

0.1 M acetic acid: 1 mg/mL (Allow to stir at room temperature 1-3 hours until dissolved.)

Descrição geral

All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type I collagen differs from other collagens by its low lysine hydroxylation and low carbohydrate composition. Col1a1 (collagen, type I, α1) or collagen is a major structural human protein, which assembles in the form of fibrils. It is a very long, thin and the most abundant protein found in the human body. It is a supercoiled right-helix of three left-handed polypeptide chains. These chains are composed of ~1040 amino acids, which are essentially repeats of three amino acids -(Gly-X-Y)n. Gly is glycine and X and Y can be any amino acids, but in humans are usually proline and hydroxyproline, respectively.

Aplicação

Collagen from rat tail is used for the following applications:

  • Immunohistochemistry
  • Cellular activity assays
  • Used in generation of dorsal root ganglion (DRG) explant cultures
  • Used as one of the components during the preparation of the functionalized surface (in NMR setup)
  • Used in cell culture (the glass coverslips were coated with nanowires at high concentrations mixed with collagen)
  • Used for biofunctionalization of the microchannels

Ações bioquímicas/fisiológicas

Collagen is an essential ingredient of connective tissue. Studies in a Chinese family show that mutation in COL1A1 (collagenase type I) is linked with type I osteogenesis imperfecta. Collagen is linked with subchondral turnover of bone, and might have potential as marker to determine the state of joint space narrowing and osteophytes in osteoarthritis.Collagen from rat tail is intended to produce thin layer coatings on tissue culture plates to facilitate attachment of anchorage-dependent cells, recommended for use at 6-10 μg/cm2. It is NOT intended for production of 3-D gels. Type I collagen is often used in cell culture as an attachment substratum with myoblasts, spinal ganglia, hepatocytes, embryonic lung, heart explants, fibroblasts, endothelial cells, and islet cells have all been cultured successfully on films or gels of type I collagen. Collagen type I may also be used in research of Idiopathic pulmonary fibrosis (IPF), studies on the effect of ER stress IPF on lung fibroblasts. Collagen in acidic solution can produce three dimensional scaffolding with use in bioengineering and cell culture applications.

Nota de preparo

Product is clear to hazy colorless solution with a few insolubles at 1 mg/ml in water with 2 μl acetic acid (or 0.1 N acetic acid). The insolubles can be removed by settling or centrifugation.

Outras notas

O colágeno é classificado em diversos tipos estrutural e geneticamente distintos. Usamos a nomenclatura proposta por Bornstein e Traub. Não confunda as designações de tipos da Sigma com tipos de classificação de colágeno reconhecidos.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Os números dos produtos são combinados com os tamanhos/quantidades da embalagem quando exibidos no site (exemplo: T1503-25G). Por favor, certifique-se você insere APENAS o número do produto no campo Número do produto (exemplo: T1503).

Exemplo

T1503
Número do produto
-
25G
Tamanho/quantidade da embalagem

Exemplos adicionais:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

inserir como 1.000309185)

Está com problemas? Sinta-se à vontade para entrar em contato Assistência Técnica para obter assistência

Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Produtos Aldrich

  • Se encontrar um número de lote como TO09019TO - insira o número do lote 09019TO sem as duas primeiras letras.

  • Se encontrar um número de lote com um código de preenchimento, como 05427ES-021 - insira o número do lote 05427ES sem o código de preenchimento -021.

  • Se encontrar um número de lote com um código de preenchimento como STBB0728K9 - digite o número do lote STBB0728 sem o código de preenchimento K9.

Não encontra o que está procurando?

Em alguns casos, um COA pode não estar disponível on-line. Se a sua pesquisa não encontrou o COA, você pode solicitar um.

Solicitar COA

Os clientes também visualizaram

Slide 1 of 7

1 of 7

Collagen human Bornstein and Traub Type I, acid soluble, powder, ~95% (SDS-PAGE)

Sigma-Aldrich

C5483

Collagen human

SAFC

SAFC

125-50

Collagen Coating Solution (50 ML)

Collagen from bovine achilles tendon powder, suitable for substrate for collagenase

Sigma-Aldrich

C9879

Collagen from bovine achilles tendon

Collagen from chicken sternal cartilage Type II (Miller), powder, BioReagent, suitable for cell culture

Sigma-Aldrich

C9301

Collagen from chicken sternal cartilage

Sigma-Aldrich

Sigma-Aldrich

C5608

Collagen from rabbit skin

Collagen from human placenta Bornstein and Traub Type IV, powder, BioReagent, suitable for cell culture

Sigma-Aldrich

C5533

Collagen from human placenta

H P Huinink et al.
Magnetic resonance in medicine, 59(6), 1282-1286 (2008-04-19)
A major application of molecular MR imaging is receptor mapping of cells lining blood vessels with targeted contrast agents. Since these agents accumulate at interfaces, knowledge of their influence on the relaxation process in this specific configuration is a prerequisite
K Makibayashi et al.
The American journal of pathology, 158(5), 1733-1741 (2001-05-05)
OCT (22-oxa-calcitriol), a vitamin D analog, has been reported to show strong inhibitory effects on mesangial cell proliferation in vitro. In the present study, we report a study of the effect of OCT on anti-thy-1 glomerulonephritis. Both OCT and 1,25(OH)(2)D(3)
Shali Chen et al.
Investigative ophthalmology & visual science, 53(13), 8333-8343 (2012-11-15)
Diabetic retinopathy entails proliferation of vascular endothelial cells (ECs) and unregulated angiogenesis. We have previously shown that ECs increase the expression of an embryonic variant of fibronectin (FN), called extra domain-B FN (ED-B FN) in response to high glucose. We
M A Fallah et al.
Biomicrofluidics, 7(4), 44124-44124 (2014-01-10)
The role of von Willebrand factor (VWF) as a shear stress activated platelet adhesive has been related to a coiled-elongated shape conformation. The forces dominating this transition have been suggested to be controlled by the proteins polymeric architecture. However, the
Hui Zhang et al.
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics, 31(6), 730-732 (2014-12-03)
To detect mutation of COL1A1 gene in a Chinese family affected with type I osteogenesis imperfecta (OI) and to provide prenatal diagnosis for a fetus at 17th gestational week. Polymerase chain reaction, DNA sequencing and restriction endonuclease analysis were used

Artigos

The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica