bovine (calf) skin
solution (0.1% solution in 0.1 M acetic acid)
pkg of 20 mL
not available in Canada (at this time; for questions or status updates, please email us at firstname.lastname@example.org)
(0.1% solution in 0.1 M acetic acid)
cell culture | mammalian: suitable
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12 - Non Combustible Liquids
Insira o número de lote para pesquisar o Certificado de análise (COA).
Insira o número de lote para pesquisar o Certificado de origem (COO).
We recommend transferring the collagen solution to a glass bottle with a screw cap and carefully layering chloroform at the bottom. The amount of chloroform to use should be ~10% of the volume of collagen solution. DO NOT SHAKE OR STIR. Allow to stand overnight at 2-8 °C. Aseptically remove the top layer containing the collagen solution to a new bottle. We do not recommend sterilizing the collagen solution by membrane filtration. We have found substantial protein loss by this method.
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
This product is NOT intended to produce 3-D (thick) gels, since the solution will not gel. This may be due to gamma-irradiation of the powder during the production process. We recommend using Product No C4243, which is tested and found to be suitable for 3-D gel formation.
C8919, Collagen Solution from calf skin, is prepared by allowing irradiated sterile collagen strands to mix with sterile filtered 0.1N acetic acid over several hours. The collagen dissolves to an opalescent solution that is aseptically dispensed while stirring. No further filtration is necessary. Due to cross-linkage and sequence variability, as well as structural changes that can occur during isolation and purification, Collagen Type I (Bornstein and Traub) is a heterogeneous mixture rather than a pure compound. This variability, along with slight differences in the raw material, lends itself to shifts in the opacity of the solution from lot to lot. This product is tested for sterility prior to release for sale to our customers. A milky or particulate appearance is not indicative of microbial contamination and does not affect product use. .
A protocol for coating cultureware can be found on the product information sheet (under Documents, above).
We make no recommendations about storage of plates after coating. Since factors such as humidity may impact on longevity of coated plates, customers should make their own determination as to whether plates can be stored and under which conditions. We used to offer SigmaScreen collagen coated plates as Product No. S3315. For optimal performance of that product, we recommend that the unopened product should be stored in a dry place at 2-8°C. We found that the S3315 could be stored at room temperature for up to three months, and that it should never be exposed to temperatures above 50°C.
This can occur but does not harm the product. When warmed to room temperature, the product will liquify. Please make sure it is well mixed prior to dilution for coating cultureware.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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