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C8919

Sigma-Aldrich

Collagen from calf skin

Bornstein and Traub Type I, (0.1% solution in 0.1 M acetic acid), aseptically processed, BioReagent, suitable for cell culture

Número CAS:
Número MDL:
NACRES:
NA.75

fonte biológica

bovine (calf) skin

Nível de qualidade

esterilidade

aseptically processed

linha de produto

BioReagent

forma

solution (0.1% solution in 0.1 M acetic acid)

embalagem

pkg of 20 mL

availability

not available in Canada (at this time; for questions or status updates, please email us at antibody.canada@sial.com)

concentração

(0.1% solution in 0.1 M acetic acid)

technique(s)

cell culture | mammalian: suitable

cobertura de superfície

6‑10 μg/cm2

nº de adesão UniProt

enviado em

wet ice

temperatura de armazenamento

2-8°C

Gene Information

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Sigma-Aldrich

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C8919

Collagen from calf skin

Collagen from chicken sternal cartilage Type II (Miller), powder, BioReagent, suitable for cell culture

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C9301

Collagen from chicken sternal cartilage

Sigma-Aldrich

Sigma-Aldrich

CC050

Human Collagen Type I

Sigma-Aldrich

Sigma-Aldrich

CC077

Human Collagen Type V

form

solution (0.1% solution in 0.1 M acetic acid)

form

powder

form

liquid

form

liquid

packaging

pkg of 20 mL

packaging

glass bottle of 100 mg, poly bottle of 25 mg, glass bottle of 5 mg

packaging

-

packaging

-

concentration

(0.1% solution in 0.1 M acetic acid)

concentration

60-80% (biuret)

concentration

1 mg/mL

concentration

1 mg/mL

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

P02453, P02459

UniProt accession no.

P02460

UniProt accession no.

P02452

UniProt accession no.

P20908

Descrição geral

Collagen type I is a component of skin, bone, tendon, and other fibrous connective tissues. It consists of repeating triplet amino acids glycine, proline and hydroxyproline. Collagen is a left handed helix with three polypeptide chains. It is a component of extracellular matrix and as close to 28 types are present in bovine.

Aplicação

Collagen from calf skin has been used:
  • for pre-coting glass slides for immunofluorescence studies
  • as a cell adhesion factor and modification of poly(vinylidene fluoride-trifluoroethylene) (P(VDF-TrFE)) films for neuron culture
  • for coating culture dishes for murine embryonic fibroblasts culture

This product is intended to produce thin layer coatings on tissue culture plates to facilitate attachment of anchorage-dependent cells, recommended for use at 6-10 μg/cm2. It is NOT intended for production of 3-D gels. Type I collagen is often used in cell culture as an attachment substratum with myoblasts, spinal ganglia, hepatocytes, embryonic lung, heart explants, fibroblasts, endothelial cells, and islet cells have all been cultured successfully on films or gels of type I collagen. Collagen type I may also be used in research of Idiopathic pulmonary fibrosis (IPF), studies on the effect of ER stress IPF on lung fibroblasts. Collagen in acidic solution can produce three dimensional scaffolding with use in bioengineering and cell culture applications.

Ações bioquímicas/fisiológicas

Collagen type I on heat denaturation results in disruption of triple helix to a randomly coil. Mutations in the collagen gene are implicated in a variety of cattle diseases. It has applications in food and cosmetics. Collagen is used as a biomaterial and as a tissue scaffold in tissue engineering. Collagen type I fiber assembly is very crucial for physiological processes and cellular signaling.

Componentes

All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type I collagen differs from other collagens by its low lysine hydroxylation and low carbohydrate composition.

Nota de preparo

This product is a 0.1% (1mg/ml) solution of calf skin collagen 0.1 M acetic acid. The product′s appearance is turbid, milky white solution. It is recommended that dilutions of product are prepared in sterile water. Please refer to information given on the product information sheet prior to introduction of product to cells and media.

Outras notas

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Código de classe de armazenamento

12 - Non Combustible Liquids

WGK

nwg

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable


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1000309185

insira como1.000309185)

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Bovine Collagen Solution

Extracellular Collagen VI Has Prosurvival and Autophagy Instructive Properties in Mouse Fibroblasts
Castagnaro S, et al.
Frontiers in Physiology, 9(3), 1129-1129 (2018)
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