Merck
All Photos(2)

C9080

Sigma-Aldrich

Monoclonal Anti-Vimentin−Cy3 antibody produced in mouse

clone V9, purified immunoglobulin, buffered aqueous solution

Synonym(s):
Monoclonal Anti-Vimentin
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

200

conjugado

CY3 conjugate

forma do anticorpo

purified immunoglobulin

antibody product type

primary antibodies

clone

V9, monoclonal

forma

buffered aqueous solution

peso molecular

antigen ~58 kDa

species reactivity

pig, canine, feline, hamster, rabbit, gerbil, monkey, bovine, chicken, human, horse, rat

technique(s)

direct immunofluorescence: 1:200 using cultured cells
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 using human tonsil
immunohistochemistry (frozen sections): suitable using human tonsil

Isotipo

IgG1

nº de adesão UniProt

enviado em

wet ice

temperatura de armazenamento

2-8°C

Gene Information

human ... VIM(7431)
rat ... Vim(81818)

Looking for similar products? Visit Product Comparison Guide

Descrição geral

Monoclonal Anti-Vimentin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Vimentin is expressed in breast myoepithelial cells,[1] osteocytes, Langerhans cells of the skin, Schwann cells and astrocytes.
Vimentin is one of the five major groups of intermediate filaments with a molecular mass of 57 kDa.[2] Intermediate filaments (IFs) with characteristic 10 nm diameter are a distinct class of molecularly heterogenous cytoskeletal filaments defined by ultrastructural, immunological and biochemical criteria. IFs differ significantly from the other cytoskeletal elements of the cell, namely microtubules and microfilaments and are components of most eukaryotic cells.

Especificidade

The antibody localizes vimentin in fibroblasts, endothelial cells, lymphoid tissue and melanocytes in immunohistochemical staining. It also stains vimentin in sarcomas, lymphomas, and melanomas.

Imunogênio

pig eye lens vimentin.

Aplicação

Monoclonal Anti-Vimentin-Cy3 antibody produced in mouse has been used in:
  • immunofluorescence and confocal microscopy[3]
  • cytoskeleton staining[4]
  • double labeling experiments
  • immunohistochemical
  • immunocytochemical localozation

Ações bioquímicas/fisiológicas

Cy3 Monoclonal Anti-Vimentin reacts with normal and pathological tissue of mesenchymally-derived cells, lens tissue and various cultured cells. It localizes vimentin in fibroblasts, lipocytes, endothelial cells, some lymphoid cells, melanocytes,[5] macrophages, and chondrocytes. The antibody stains tumors derived from these cells, including sarcomas, most lymphomas, melanomas and their metastatic lesions. Co-expression of vimentin with keratin was described in most carcinomas of certain sites (e.g., kidney and thyroid) and certain carcinomas of other sites (e.g., breast). [6]
Vimentin was found to be important for stabilizing the architecture of the cytoplasm. It was found, that activated macrophages secrete vimentin into the extracellular space in vitro.[2]

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Armazenamento e estabilidade

Store at 2-8 °C. Protect from prolonged exposure to light. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Informações legais

Cy is distributed under license from Amersham Biosciences Limited.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de classe de armazenamento

12 - Non Combustible Liquids

WGK

nwg

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service