Merck
All Photos(3)

C9282

Sigma-Aldrich

Sodium cholate hydrate

suitable for cell culture, BioReagent

Synonym(s):
3α,7α,12α-Trihydroxy-5β-cholan-24-oic acid sodium salt, Cholalic acid sodium salt
Empirical Formula (Hill Notation):
C24H39NaO5 · xH2O
CAS Number:
Molecular Weight:
430.55 (anhydrous basis)
Beilstein:
3582354
Número EC:
Número MDL:
ID de substância PubChem:
NACRES:
NA.75

fonte biológica

bovine bile
ovine bile

Nível de qualidade

200

descrição

anionic

linha de produto

BioReagent

teor

≥99.0% (HPLC)

forma

powder

peso molecular

micellar avg mol wt 900-1300

número de agregação

2-3

technique(s)

cell culture | mammalian: suitable

CMC

9-15 mM (20-25°C)

solubilidade

water: 100 mg/mL, clear, colorless to light yellow

HLB

18

SMILES string

O.[Na+].C[C@H](CCC([O-])=O)C1CCC2C3[C@H](O)CC4C[C@H](O)CC[C@]4(C)C3C[C@H](O)[C@]12C

InChI

1S/C24H40O5.Na.H2O/c1-13(4-7-21(28)29)16-5-6-17-22-18(12-20(27)24(16,17)3)23(2)9-8-15(25)10-14(23)11-19(22)26;;/h13-20,22,25-27H,4-12H2,1-3H3,(H,28,29);;1H2/q;+1;/p-1/t13-,14+,15-,16-,17+,18+,19-,20+,22+,23+,24-;;/m1../s1

InChI key

MUVVIYFKOVLQHL-RCVKHMDESA-M

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Descrição geral

Cholate is a primary bile acid produced from cholesterol in the liver. It is an anionic detergent synthesized from cholesterol in the presence of sterol 12α-hydroxylase (CYP8B1).

Aplicação

Cholate is a bile acid produced in liver during cholesterol breakdown. Cholate downregulates cholesterol-7-α-hydroxylase (rate-limiting step in bile acid synthesis). Cholate, an anionic detergent, is used alone and in combination with urea to extract and reconstitute membrane proteins and protein complexes.
Sodium cholate hydrate has been used:
  • as a bile acid to induce bile acid-responsive (BEAR) transcription system,
  • as a component of Campylobacter defined broth (CDB) to test its effect on stress response in Campylobacter jejuni{52
  • to test its sensitivity towards the bile acid sensor

Embalagem

25, 100 g in poly bottle

Ações bioquímicas/fisiológicas

Reduction in 12-α hydroxylase activity leads to low levels of cholic acid in liver disease.

Código de classe de armazenamento

13 - Non Combustible Solids

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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