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C9791

Sigma-Aldrich

Collagen from calf skin

Bornstein and Traub Type I, solid, BioReagent, suitable for cell culture

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Sinônimo(s):
Type I collagen
Número CAS:
Número CE:
Número MDL:
NACRES:
NA.75

fonte biológica

bovine (calf) skin

linha de produto

BioReagent

forma

solid

embalagem

poly bottle of 10 mg
poly bottle of 100 mg
poly bottle of 250 mg
poly bottle of 50 mg

availability

not available in Canada (at this time; for questions or status updates, please email us at antibody.canada@sial.com)

technique(s)

cell culture | mammalian: suitable

cobertura de superfície

6‑10 μg/cm2

solubilidade

0.1 M acetic acid: 1 mg/mL (Allow to stir at room temperature 1-3 hours until dissolved.)

nº de adesão UniProt

Especificidade de ligação

Peptide Source: Fibronectin

Peptide Source: Laminin

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

Gene Information

bovine ... COL1A1(282187)

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1 of 4

Este Item
C8919C2124C9301
Collagen from calf skin Bornstein and Traub Type I, solid, BioReagent, suitable for cell culture

Sigma-Aldrich

C9791

Collagen from calf skin

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C8919

Collagen from calf skin

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C2124

Collagen solution from bovine skin

Collagen from chicken sternal cartilage Type II (Miller), powder, BioReagent, suitable for cell culture

Sigma-Aldrich

C9301

Collagen from chicken sternal cartilage

form

solid

form

solution (0.1% solution in 0.1 M acetic acid)

form

liquid

form

powder

packaging

poly bottle of 10 mg, poly bottle of 100 mg, poly bottle of 250 mg, poly bottle of 50 mg

packaging

pkg of 20 mL

packaging

pkg of 50 mL

packaging

glass bottle of 100 mg, poly bottle of 25 mg, glass bottle of 5 mg

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

surface coverage

6‑10 μg/cm2

surface coverage

6‑10 μg/cm2

surface coverage

6‑10 μg/cm2

surface coverage

6‑10 μg/cm2

solubility

0.1 M acetic acid: 1 mg/mL (Allow to stir at room temperature 1-3 hours until dissolved.)

solubility

-

solubility

-

solubility

acetic acid: 0.5-2.0 mg/mL (Dissolve for several hours at 2-8 °C, occasionally swirling.)

Descrição geral

All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type I collagen differs from other collagens by its low lysine hydroxylation and low carbohydrate composition. Collagen type I is a component of skin, bone, tendon, and other fibrous connective tissues. It is a left handed helix with three polypeptide chains and contains repeating units of glycine, proline and hydroxyproline amino acids. It is a component of extracellular matrix and close to 28 types is present in bovine.

Aplicação

Collagen from calf skin has been used:
  • as a component of collagen gel matrix for culturing preantral follicles
  • as a component of Roswell Park Memorial Institute, for culturing gilthead seabream kidney leukocytes and macrophages and acidophilic granulocytes
  • to coat transwells prior to seeding of epithelial cell culture

This product is intended to produce thin layer coatings on tissue culture plates to facilitate attachment of anchorage-dependent cells, recommended for use at 6-10 μg/cm2. It is NOT intended for production of 3-D gels. Type I collagen is often used in cell culture as an attachment substratum with myoblasts, spinal ganglia, hepatocytes, embryonic lung, heart explants, fibroblasts, endothelial cells, and islet cells have all been cultured successfully on films or gels of type I collagen. Collagen type I may also be used in research of Idiopathic pulmonary fibrosis (IPF), studies on the effect of ER stress IPF on lung fibroblasts. Collagen in acidic solution can produce three dimensional scaffolding with use in bioengineering and cell culture applications.

Ações bioquímicas/fisiológicas

Collagen from calf skin is intended to produce thin layer coatings on tissue culture plates to facilitate attachment of anchorage-dependent cells, recommended for use at 6-10 μg/cm2. It is NOT intended for production of 3-D gels. Type I collagen is often used in cell culture as an attachment substratum with myoblasts, spinal ganglia, hepatocytes, embryonic lung, heart explants, fibroblasts, endothelial cells, and islet cells have all been cultured successfully on films or gels of type I collagen. Collagen type I may also be used in research of Idiopathic pulmonary fibrosis (IPF), studies on the effect of ER stress IPF on lung fibroblasts. Collagen in acidic solution can produce three dimensional scaffolding with use in bioengineering and cell culture applications. Mutations in collagen encoding proteins are implicated cattle diseases. Collagen type I on heat denaturation results in disruption of triple helix to a randomly coils. It has applications in food and cosmetics and is used as biomaterial in in tissue engineering.

Componentes

All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type I collagen differs from other collagens by its low lysine hydroxylation and low carbohydrate composition.

Nota de preparo

This product was prepared by a modification of Gallop, P.M. and Seifter, S., Meth. Enzymol., VI, 635 (1963). It is soluble at 1 mg/mL in .1 M acetic acid and should be stirred at room temperature for 1-3 hours until dissolved.

Outras notas

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


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Bals R, et al.
Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society, 3, 49-51 (2004)
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