P3X63Ag8.653-TurboDoma

03092502

Propriedades

• fonte biológica: mouse blood
• modo de crescimento: Suspension
• cariótipo: Not specified
• morfologia: Lymphoblast
• produtos: Not specified
• receptores: Not specified
• technique(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• enviado em: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse BALB/c myeloma, serum-free

Descrição de linhagem celular

P3X63Ag8.653 cell line (Sigma Catalogue number. 85011420) adapted to grow in the chemically defined protein- and peptide-free medium TurboDoma^™. Suitable for use as a fusion parent for serum-free generation of hybridomas.

Aplicação

Fusion partner for generation of serum-free hybridomas.

Meio de cultura

TurboDoma^™ medium + 4mM Glutamine. When freezing cells down use TurboDoma medium (50:50 conditioned medium:fresh medium) + 4mM Glutamine + 10% DMSO.

Rotina de subcultura

Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100g for 2-3 minutes to pellet cells and seed at a relatively high density of 5-7 x 10⁵ cells/ml. Leave culture flask upright and observe regularly until viable proliferating cells are seen. Once established use a split ratio of 1:2 approximately every 4 to 5 days; 5% CO₂; 37°C.

Outras notas

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Informações legais

TurboDoma is a trademark of Cell Culture Technologies