Merck
All Photos(1)

AC2

86061902

All Photos(1)

fonte biológica

mouse blood

modo de crescimento

Suspension

cariótipo

Not specified

morfologia

Lymphoblast

produtos

Not specified

receptores

Interleukin 3 (IL-3)

technique(s)

cell culture | mammalian: suitable

enviado em

dry ice

temperatura de armazenamento

−196°C

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This Item
SML0129CB_85040103CB_90071812
AC2 86061902

AC2

AC-265347 ≥98% (HPLC)

Sigma-Aldrich

SML0129

AC-265347

BHK/AC9 85040103

BHK/AC9

CTAC 90071812

CTAC

biological source

mouse blood

biological source

-

biological source

hamster kidney

biological source

dog thyroid

growth mode

Suspension

growth mode

-

growth mode

Suspension

growth mode

Adherent

karyotype

Not specified

karyotype

-

karyotype

2n = 44, pseudodiploid

karyotype

2n = 78

morphology

Lymphoblast

morphology

-

morphology

Not specified

morphology

Epithelial-like

products

Not specified

products

-

products

Not specified

products

Not specified

receptors

Interleukin 3 (IL-3)

receptors

-

receptors

Not specified

receptors

Not specified

Origem de linhagem celular

Mouse lymphoblast

Descrição de linhagem celular

Derived from a mouse basophil clone and is dependent on Interleukin-3 (IL-3) for growth. IL-3 may be added in the form of Wehi conditioned media (10-20%). Culture densities between 10,000 and 100,000 cells/ml is necessary to retain cells in exponential growth phase.

Aplicação

Growth factor studies, IL-2 assays

Meio de cultura

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Maintain cultures between 10,000 to 100,000 cells/ml; 5% CO2; 37°C. Cells will rapidly die at high densities above 5x100,000 cells/ml. Culture very sensitive to agitation.

Outras notas

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