Merck
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D5025

Sigma-Aldrich

Deoxyribonuclease I from bovine pancreas

Type IV, lyophilized powder, ≥2,000 Kunitz units/mg protein

Sinônimo(s):
Deoxyribonucleate 5′-oligonucleotido-hydrolase, DNase I
Número CAS:
Número da licença da enzima:
Número EC:
Número MDL:
NACRES:
NA.54

Nível de qualidade

300

fonte biológica

bovine pancreas

tipo

Type IV

forma

lyophilized powder

specific activity

≥2,000 Kunitz units/mg protein

peso molecular

~31 kDa

purificado por

chromatography

composição

Protein, ≥80%

solubilidade

0.15 M NaCl: soluble 5.0 mg/mL, clear, colorless

application(s)

diagnostic assay manufacturing
diagnostic assay manufacturing

atividade externa

Chymotrypsin ≤0.5%
Protease ≤0.05%
RNase ≤0.02%

enviado em

wet ice

temperatura de armazenamento

−20°C

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Aplicação

DNAse I is used to nick DNA as a first step to incorporate labeled bases into DNA. The enzyme from Sigma has been used in the processing of rat brain tissue. This study showed that axonal growth on astrocytes is not inhibited by oligodendrocytes. In another study, thawed fixed samples of E. coli were digested with DNAse I from Sigma along with other enzymes. The digestion was done before permeabilization and staining of the nucleic acids.
Deoxyribonuclease I from bovine pancreas has been used in a study to investigate a two-dimensional zymogram analysis of nucleases in Bacillus subtilis. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effects of minor and major groove-binding drugs and intercalators on the DNA association of minor groove-binding proteins RecA and deoxyribonuclease I.
Used for the removal of DNA from protein samples.

Embalagem

15000 units in glass bottle
150000, 375000, 750000 units in poly bottle

Ações bioquímicas/fisiológicas

DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+. The pH optimum is found to be between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with their molar ratios being 4:1:1. Only minor amounts of D are found. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS) and actin are known to inhibit the enzyme activity.

Definição da unidade

One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 5.0 at 25 °C, using DNA, Type I or III as substrate.

forma física

Lyophilized powder containing calcium chloride

Nota de preparo

10 mg/mL solution of DNAse I in 0.15 M NaCl may lose <10% of its activity when stored for a week in aliquots at −20 °C. The same solutions stored in aliquots at 2-8 °C can lose approximately 20% activity. It remains active for up to five hours at 60 °C between pH 5 and 7, and loses activity in <10 minutes at 68 °C. It loses activity at the rate of 6%/hour in acetate buffer (pH 5.0) and tris buffer ((pH 7.2) at 1 mg/mL concentration.

Nota de análise

Protein determined by biuret.

Código de classe de armazenamento

13 - Non Combustible Solids

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Certificado de análise

Insira o número de lote para pesquisar o Certificado de análise (COA).

Certificado de origem

Insira o número de lote para pesquisar o Certificado de origem (COO).

Informações técnicas

Mais documentos

Cotações e pedidos

Enzymes of Molecular Biology
Weir, A. F.
Methods in Molecular Biology, 16 (1993)
J W Fawcett et al.
Journal of cell science, 103 ( Pt 2), 571-579 (1992-10-01)
Axon growth in vitro may be inhibited by contact with oligodendrocytes, but most axons grow readily on the surface of astrocyte monolayers. Since both cell types are in close contact with one another in the damaged nervous system, we have
T Guindulain et al.
Applied and environmental microbiology, 63(11), 4608-4611 (1997-11-15)
Three nucleic acid dyes (SYTO-13, TOTO-1, and YOYO-1) were tested on cultures of Escherichia coli and marine prokaryote populations. These dyes stain the RNA and DNA in E. coli but only respond to DNA in marine populations, according to the
Wenzhen Zhao et al.
Molecular reproduction and development, 86(8), 984-998 (2019-05-28)
Sertoli cells are a type of nurse cell in the seminiferous epithelium that are crucial for sustaining spermatogenesis by extending nutritional and energy support to the developing germ cells. Dysfunction of Sertoli cells could cause disordered spermatogenesis and reduced fertility
Sambrook, J., and Russell, D.W.
Molecular Cloning: A Laboratory Manual, 2(2), 5-5 (2001)

Protocolos

Enzymatic Assay of Deoxyribonuclease I (EC 3.1.21.1)

To standardize a procedure for the enzymatic assay of Deoxyribonuclease I.

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