Merck
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D5758

Sigma-Aldrich

Diethyl pyrocarbonate

96% (NT)

Synonym(s):
Diethyl dicarbonate, DEPC, Diethyl oxydiformate, DEP, Ethoxyformic acid anhydride
Linear Formula:
O(COOC2H5)2
CAS Number:
Molecular Weight:
162.14
Beilstein:
637031
Número EC:
Número MDL:
ID de substância PubChem:
NACRES:
NA.77

Nível de qualidade

200

fonte biológica

synthetic (microbial fermentation/ cell culture)

teor

96% (NT)

forma

liquid

índice de refração

n20/D 1.398 (lit.)

pb

93-94 °C/18 mmHg (lit.)

densidade

1.101 g/mL at 25 °C (lit.)

enviado em

wet ice

temperatura de armazenamento

2-8°C

SMILES string

CCOC(=O)OC(=O)OCC

InChI

1S/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H3

InChI key

FFYPMLJYZAEMQB-UHFFFAOYSA-N

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Descrição geral

Diethyl Pyrocarbonate (DEPC) is a chemical use to inactivate RNase enzymes and is sensitive to moisture and pH. It decomposes at 155°C, to ethanol and carbon dioxide in aqueous solution. DEPC is also sensitive to ammonia, which causes decomposition to urethane, a possible carcinogen.

Aplicação

Diethyl pyrocarbonate has been used in PCR reaction for treating deionized water, which reduces the risk of RNA being degraded by RNases. It is also used for Dot blot hybridization, to dilute total RNA isolated from different micro-organisms.
Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.

Embalagem

5, 25, 50, 100 mL in glass bottle

Ações bioquímicas/fisiológicas

DEPC is a potent nuclease inhibitor.0.1% solution of DEPC is used to inactive RNase.
It inhibits the ryanodine binding to ryanodine/Ca2+ receptor channels in skeletal muscle in a dose and time dependent manner and increases the Ca2+ permeability of SR vesicles.
Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.

Pictogramas

Exclamation mark

Palavra indicadora

Warning

Frases de perigo

Declarações de precaução

Classificações de perigo

Acute Tox. 4 Oral

Código de classe de armazenamento

10 - Combustible liquids

WGK

WGK 3

Ponto de fulgor (ºF)

156.2 °F - closed cup

Ponto de fulgor (ºC)

69 °C - closed cup

Equipamento de proteção individual

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificate of Analysis

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Certificate of Origin

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