D5905

3,3′-Diaminobenzidine tetrahydrochloride

tablet, 10 mg substrate per tablet

• Synonym(s): 3,3′,4,4′-Biphenyltetramine tetrahydrochloride, DAB, 3,3′,4,4′-Tetraaminobiphenyl tetrahydrochloride
• Linear Formula: (NH₂)₂C₆H₃C₆H₃(NH₂)₂ · 4HCl
• Molecular Weight: 360.11
• Beilstein: 1212988
• Número EC: 231-018-9
• Número MDL: MFCD00012969
• ID de substância PubChem: 24893981
• NACRES: NA.83

Properties

• Nível de qualidade: 200
• forma: tablet
• solubilidade: water: 1 tablet/15 mL (may contain small insoluble particles)
• temperatura de armazenamento: −20°C
• SMILES string: Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2
• InChI: 1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H
• InChI key: KJDSORYAHBAGPP-UHFFFAOYSA-N

Description

Especificidade

Peroxidase substrate suitable for use in immunoblotting and immunohistological staining procedures. This substrate produces an insoluble end product that is brown in color and can be observed visually.

Aplicação

3,3′-Diaminobenzidine tetrahydrochloride has been used for immunohistochemistry.

Reagent for spectrophotometric determination of selenium.

Ações bioquímicas/fisiológicas

3,3′-Diaminobenzidine tetrahydrochloride is a chromogenic substrate, which is commonly used in immunoperoxidase tests.

Reconstituição

Dissolve 1 tablet in 15 ml of Tris-buffered saline, pH 7.6. Add 12 μl of fresh 30% hydrogen peroxide. DAB and hydrogen peroxide concentration may be adjusted to suit individual applications.

Safety Information

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H341 - H350
• Declarações de precaução: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Classificações de perigo: Carc. 1B - Muta. 2
• Código de classe de armazenamento: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)