Merck
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D5905

Sigma-Aldrich

3,3′-Diaminobenzidine tetrahydrochloride

tablet, 10 mg substrate per tablet

Synonym(s):
3,3′,4,4′-Biphenyltetramine tetrahydrochloride, DAB, 3,3′,4,4′-Tetraaminobiphenyl tetrahydrochloride
Linear Formula:
(NH2)2C6H3C6H3(NH2)2 · 4HCl
Molecular Weight:
360.11
Beilstein:
1212988
Número EC:
Número MDL:
ID de substância PubChem:
NACRES:
NA.83

Nível de qualidade

200

forma

tablet

solubilidade

water: 1 tablet/15 mL (may contain small insoluble particles)

temperatura de armazenamento

−20°C

SMILES string

Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H

InChI key

KJDSORYAHBAGPP-UHFFFAOYSA-N

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Especificidade

Peroxidase substrate suitable for use in immunoblotting and immunohistological staining procedures. This substrate produces an insoluble end product that is brown in color and can be observed visually.

Aplicação

3,3′-Diaminobenzidine tetrahydrochloride has been used for immunohistochemistry.
Reagent for spectrophotometric determination of selenium.

Ações bioquímicas/fisiológicas

3,3′-Diaminobenzidine tetrahydrochloride is a chromogenic substrate, which is commonly used in immunoperoxidase tests.

Reconstituição

Dissolve 1 tablet in 15 ml of Tris-buffered saline, pH 7.6. Add 12 μl of fresh 30% hydrogen peroxide. DAB and hydrogen peroxide concentration may be adjusted to suit individual applications.

Pictogramas

Health hazard

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Carc. 1B - Muta. 2

Código de classe de armazenamento

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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