Merck
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D7291

Sigma-Aldrich

Deoxyribonuclease I RNase-free solution from bovine pancreas

Synonym(s):
DNase I, Deoxyribonuclease I, pancreatic DNase I, pancreatic deoxyribonuclease I
CAS Number:
Enzyme Commission number:
Número EC:
Número MDL:
NACRES:
NA.53

fonte biológica

bovine pancreas

Nível de qualidade

200

grau

for molecular biology

forma

buffered aqueous glycerol solution

specific activity

10000 units/mg protein





peso molecular

29.1 kDa

nº de adesão UniProt

atividade externa

RNase, none detected

temperatura de armazenamento

−20°C

Gene Information

Descrição geral

Deoxyribonuclease I digests single- and double-stranded DNA to a mixture of mono and oligonucleotides carrying 5′phosphates and 3′OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg2+, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn2+, both strands can be cleaved.

Aplicação

Deoxyribonuclease I RNase-free solution from bovine pancreas has been used to digest DNA from various samples.
Used in molecular biology applications for removing DNA during RNA purification, for preparing DNA for nick translation, and for DNA-protein interaction analysis by footprinting methods.

Componentes

DNase I is provided in a solution of 50% (v/v) glycerol, 20 mM sodium acetate (pH 6.5), 5 mM CaCl2, and 0.1 mM PMSF.

Definição da unidade

One unit will cause a ΔA260 of 0.001 per min per mL reaction mixture using calf thymus DNA as substrate.

Outras notas

RNA treated with this DNase I should not be used to generate a cDNA library or used for RT-PCR. For these sensitive applications, it is recommended to use DNase I, Amplification Grade , Catalog Number AMP-D1.

Pictogramas

Health hazard

Palavra indicadora

Danger

Frases de perigo

Declarações de precaução

Classificações de perigo

Resp. Sens. 1

Código de classe de armazenamento

11 - Combustible Solids

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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