Merck
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D8037

Sigma-Aldrich

Driselase™ Basidiomycetes sp.

BioReagent, suitable for plant cell culture

Todas as fotos(5)
Sinônimo(s):
Driselase™ from Basidiomycetes sp.
Número CAS:
85186-71-6
Número EC:
286-055-3
Número MDL:
MFCD00130978
NACRES:
NA.72

linha de produto

BioReagent

Nível de qualidade

200

forma

powder

composição

Protein, ≥10% biuret

technique(s)

cell culture | plant: suitable

application(s)

agriculture

temperatura de armazenamento

−20°C

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Este Item
C1794D7674D5417
Driselase™ Basidiomycetes sp. BioReagent, suitable for plant cell culture

Sigma-Aldrich

D8037

Driselase™ Basidiomycetes sp.

Cellulase from Trichoderma sp. BioReagent, suitable for plant cell culture, 3-10 units/mg solid

Sigma-Aldrich

C1794

Cellulase from Trichoderma sp.

6-(γ,γ-Dimethylallylamino)purine BioReagent, suitable for plant cell culture, ≥98.5%

Sigma-Aldrich

D7674

6-(γ,γ-Dimethylallylamino)purine

Dicamba suitable for plant cell culture, BioReagent

Sigma-Aldrich

D5417

Dicamba

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

product line

BioReagent

product line

BioReagent

product line

BioReagent

product line

BioReagent

composition

Protein, ≥10% biuret

composition

-

composition

-

composition

-

Aplicação

Driselase™ Basidiomycetes sp. has been used:

  • in spheroplast preparation from Coccomyxa cells
  • in a CRISPR/Cas9-based mutagenesis protocol for Brachypodium distachyon and its allopolyploid relative, Brachypodium hybridum
  • for cell wall digestion to perform whole-mount immunolocalization of Lotus japonicus root tissue

Ações bioquímicas/fisiológicas

Driselase™ is a natural mixture of enzyme activities (fungal carbohydrates) used to digest plant cell walls to facilitate the maceration of plant materials, protoplast formation, and extraction processes. Driselase releases cell wall carbohydrates. This formulation contains enzyme activities of cellulose, endo-1,3-β-glucanase, and xylanase.

Outras notas

Crude powder containing laminarinase, xylanase and cellulase.

Informações legais

Driselase is a trademark of ASKA Animal Health Co. Ltd.

Pictogramas

Health hazard
GHS08

Palavra indicadora

Danger

Frases de perigo

H334

Declarações de precaução

P261 - P284 - P501

Classificações de perigo

Resp. Sens. 1

Código de classe de armazenamento

11 - Combustible Solids

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Exemplo

T1503
Número do produto
-
25G
Tamanho/quantidade da embalagem

Exemplos adicionais:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

insira como1.000309185)

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Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Produtos Aldrich

  • Se encontrar um número de lote como TO09019TO - insira o número do lote 09019TO sem as duas primeiras letras.

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ERB-041 ≥98% (HPLC)

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PZ0183

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Pectolyase from Aspergillus japonicus lyophilized powder, ≥0.3 units/mg solid

Sigma-Aldrich

P3026

Pectolyase from Aspergillus japonicus

Cellulase from Trichoderma sp. BioReagent, suitable for plant cell culture, 3-10 units/mg solid

Sigma-Aldrich

C1794

Cellulase from Trichoderma sp.

Dieter Hackenberg et al.
Plant physiology, 172(2), 1154-1166 (2016-08-24)
In this study, we report the functional characterization of heterotrimeric G-proteins from a nonvascular plant, the moss Physcomitrella patens. In plants, G-proteins have been characterized from only a few angiosperms to date, where their involvement has been shown during regulation
M C Ralet et al.
Carbohydrate research, 263(2), 227-241 (1994-10-17)
Cell walls from sugar-beet pulp contain some feruloyl groups linked to the pectic neutral side-chains. Enzymic as well as chemical hydrolysis of the pulp yielded a series of feruloylated oligosaccharides, which have been purified by Sephadex LH-20 and Biogel P-2
D T Kaplan et al.
Journal of nematology, 22(3), 399-406 (1990-07-01)
Radopholus spp. were reared in carrot tissue culture via established procedures, with slight modification. Several plant tissue maceration enzymes and flotation media (salts and sucrose) were evaluated with regard to nematode toxicity and extraction efficiency. Best extraction of viable nematodes
D P Blowers et al.
Plant physiology, 86(2), 505-509 (1988-02-01)
Plasma membrane vesicles from wild carrot cells grown in suspension culture were isolated by aqueous two-phase partitioning, and ATP-dependent phosphorylation was measured with [gamma-(32)P]ATP in the presence and absence of calcium. Treatment of the carrot cells with the cell wall
T Ishii et al.
Carbohydrate research, 248, 179-190 (1993-10-04)
Hydrolysis of spinach-leaf cell walls with Driselase (a fungal enzyme preparation) released two arabino-oligosaccharides and one galactobiose, each carrying a ferulic acid moiety. The oligosaccharides were characterized by NMR spectroscopy, methylation analysis, and FABMS. They were O-(2-O-trans-feruloyl-alpha-L-arabinofuranosyl)-(1-->5)-L-arabinof uranose, O-(6-O-trans-feruloyl-beta-D-galactopyranosyl)-(1-->4)-D-galactopy ranose
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