Merck
All Photos(6)

D8418

Sigma-Aldrich

Dimethyl sulfoxide

for molecular biology

Synonym(s):
Methyl Sulfoxide, Methylsulfinylmethane, Molecular Biology Grade DMSO, DMSO
Linear Formula:
(CH3)2SO
CAS Number:
Molecular Weight:
78.13
Beilstein:
506008
Número EC:
Número MDL:
ID de substância PubChem:
NACRES:
NA.52

grau

for molecular biology

Nível de qualidade

200

densidade de vapor

2.7 (vs air)

pressão de vapor

0.42 mmHg ( 20 °C)

linha de produto

BioReagent

teor

≥99.9%

forma

liquid

temperatura de autoignição

573 °F

prazo de validade

Recommended retest period - 2 years

Lim. expl.

42 %, 63 °F

technique(s)

DNA sequencing: suitable
PCR: suitable
transfection: suitable

Impurezas

≤0.001 meq/g Titratable acid
≤0.1% water (Karl Fischer)

cor

colorless

índice de refração

n20/D 1.479 (lit.)

pb

189 °C (lit.)

pf

16-19 °C (lit.)

solubilidade

H2O: miscible (completely)

densidade

1.10 g/mL (lit.)

absorção

passes test

adequação

suitable for molecular biology

atividade externa

DNase and RNase, none detected

temperatura de armazenamento

room temp

SMILES string

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

Descrição geral

Features and Benefits:

  • Suitable for Molecular Applications
  • Nuclease-Free DMSO
  • DNase-Free, RNase-Free, Protease-Free, Phosphatase-Free
  • Molecular Biology Grade DMSO

Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals.
This product is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the presence of nucleases and proteases.

Aplicação

DMSO (dimethyl sulfoxide) is a highly polar, aprotic organic solvent with many applications in organic chemistry and molecular biology. DMSO is routinely used in

  • polymerase chain reaction (PCR)
  • amplification of cDNA libraries
  • DNA sequencing
  • column-loading buffers for poly(A)+ RNA selection
  • buffers for the transformation of competent E. coli
  • transfection protocols.

Dimethyl sulfoxide has been used-

  • as an oligonucleotide solvent in array spotting[1]
  • as a solvent during miRNA microarray analysis and quantitative real-time PCR (qRT-PCR)
  • for cell lysis during MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay
  • for the preparation of the stock solution during MMP (mitochondrial membrane potential)cytofluorimetric assessment
  • in the storage of human and animal cell lines and bacteriophage λ as a cryoprotective agent.
To prepare a sterile filtered DMSO solution, it is recommended to use a PTFE or nylon membrane. Cellulose acetate membranes are not recommended.

Embalagem

50, 100, 250, 500 mL in glass bottle
1 L in glass bottle

Características e benefícios

This product is free from DNases, RNases, phosphatases and proteases.

Atenção

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Código de classe de armazenamento

10 - Combustible liquids

WGK

WGK 1

Ponto de fulgor (ºF)

188.6 °F - closed cup

Ponto de fulgor (ºC)

87 °C - closed cup

Equipamento de proteção individual

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service