Merck
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D8764

Sigma-Aldrich

Deoxyribonuclease II from bovine spleen

Type V, essentially salt-free, lyophilized powder, ≥1,000 units/mg protein

Synonym(s):
DNase II, Deoxyribonucleate 3′-oligonucleotido-hydrolase
CAS Number:
Enzyme Commission number:
Número EC:
Número MDL:
NACRES:
NA.54

tipo

Type V

Nível de qualidade

200

forma

essentially salt-free, lyophilized powder

specific activity

≥1,000 units/mg protein

peso molecular

38 kDa

atividade externa

RNase ≤0.1%

temperatura de armazenamento

−20°C

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Aplicação

Deoxyribonuclease II from bovine spleen has been used:
  • as a component of digestion mix to isolate immune cells from colon samples
  • to digest retinal explants for single-cell suspension preparation
  • for isolation of stromal cells from human colon tissue
  • in the dissociation medium during the preparation of embryonic cardiac myocytes from rat heart

Embalagem

30000, 150000, 300000 units in poly bottle

Ações bioquímicas/fisiológicas

Deoxyribonuclease II, also called as acid DNAse, hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3′-phosphates. in vitro, its optimum pH range is 4.5 - 5.0. It also acts upon p-nitrophenyl-phosphodiesters at pH 5.6 - 5.9. The molecular weight is approximately 38,000 Da.

Definição da unidade

One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25 °C; [Mg2+] = 0.83 mM

Código de classe de armazenamento

11 - Combustible Solids

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Certificate of Analysis

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Certificate of Origin

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Technical Information

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