Merck
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D9163

Sigma-Aldrich

DL-Dithiothreitol

suitable for electrophoresis, ≥99%

Sinônimo(s):
threo-1,4-Dimercapto-2,3-butanediol, DTT, Cleland’s reagent
Fórmula linear:
HSCH2CH(OH)CH(OH)CH2SH
Número CAS:
Peso molecular:
154.25
Beilstein:
1719757
Número EC:
Número MDL:
ID de substância PubChem:
NACRES:
NB.22

Nível de qualidade

200

teor

≥99%

forma

powder

reaction suitability

reagent type: reductant

technique(s)

electrophoresis: suitable

pf

40-44 °C
41-44 °C (lit.)

solubilidade

H2O: 50 mg/mL, clear, colorless to very faintly yellow

traços de cátion

heavy metals (as Pb): ≤10 ppm

absorção

≤0.04 at 280 at 0.02 M

temperatura de armazenamento

2-8°C

SMILES string

O[C@H](CS)[C@H](O)CS

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

InChI key

VHJLVAABSRFDPM-QWWZWVQMSA-N

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Embalagem

1, 5, 25 g in poly bottle

Aplicação

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Nota de análise

Tested for use in reduced SDS-PAGE

Pictogramas

CorrosionExclamation mark

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

Código de classe de armazenamento

11 - Combustible Solids

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificado de análise

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Certificado de origem

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