D9542

DAPI

for nucleic acid staining

• Synonym(s): 4′,6-Diamidino-2-phenylindole dihydrochloride, 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride, DAPI dihydrochloride
• Empirical Formula (Hill Notation): C₁₆H₁₅N₅ · 2HCl
• CAS Number: 28718-90-3
• Molecular Weight: 350.25
• Beilstein: 4894417
• Número EC: 249-186-7
• Número MDL: MFCD00012681
• ID de substância PubChem: 24894305
• NACRES: NA.52

Properties

• grau: for molecular biology
• Nível de qualidade: 300
• teor: ≥98% (HPLC)
• forma: powder
• technique(s): transfection: suitable
• solubilidade: H₂O: 20 mg/mL (heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.); PBS: insoluble
• ε (extinction coefficient): 30 at 263 nm in H₂O at 1 mM
• fluorescência: λ_ex 340 nm; λ_em 488 nm (nur DAPI); λ_ex 364 nm; λ_em 454 nm (DAPI-DNA-Komplex)
• adequação: suitable for fluorescence
• temperatura de armazenamento: 2-8°C
• SMILES string: Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
• InChI: 1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
• InChI key: FPNZBYLXNYPRLR-UHFFFAOYSA-N

Description

Descrição geral

DAPI (4′,6-Diamidino-2-phenylindole dihydrochloride) is a cell permeable, fluorescent dye that binds to DNA.

Aplicação

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

Suitable for

• DNA staining in agarose gels
• analysis of changes in DNA during apoptosis
• detection of mycoplasma
• photofootprinting of DNA
• immunofluorescent staining of cells

DAPI has been used:-

• in rapid monitoring of microbial contamination
• in chromosomal banding technique
• in detection of apoptotic cells
• in fluorescence microscopy to track the DisA (DNA integritiy scanning protein) movement on Bacillus subtilis DNA
• to stain mature pollen grains(0.5 mg/ml)

Embalagem

1, 5, 10, 50 mg in glass bottle

Ações bioquímicas/fisiológicas

Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

Atenção

Protect from light.

Safety Information

• Pictogramas: GHS07
• Palavra indicadora: Warning
• Frases de perigo: H315 - H317 - H335
• Declarações de precaução: P261 - P264 - P271 - P272 - P280 - P302 + P352
• Classificações de perigo: Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3
• Órgãos-alvo: Respiratory system
• Código de classe de armazenamento: 11 - Combustible Solids
• WGK: WGK 3
• Ponto de fulgor (ºF): Not applicable
• Ponto de fulgor (ºC): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)