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E1250

Sigma-Aldrich

Elastase from porcine pancreas

Type I, ≥4.0 units/mg protein

Sinônimo(s):
Elastase from hog pancreas, Pancreatopeptidase E
Número CAS:
Número da licença da enzima:
Número EC:
Número MDL:
NACRES:
NA.54

fonte biológica

Porcine pancreas

Nível de qualidade

200

tipo

Type I

forma

suspension

specific activity

≥4.0 units/mg protein

peso molecular

25.9 kDa

contém

0.1% thymol

concentração

0.5-15.0 mg/mL in water

atividade externa

trypsin ≤50 BAEE units/mg protein

temperatura de armazenamento

2-8°C

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Categorias relacionadas

Aplicação

Elastase from porcine pancreas has been used in a study to investigate the design, synthesis and evaluation of biomimetic affinity ligands for elastases. Elastase from porcine pancreas has also been used in a study to investigate the purification and partial characterization of the pancreatic proteolytic enzymes trypsin, chymotrypsin, and elastase.
The enzyme from Sigma has been used in the development of elastase-perfused animal model . This study determined if tobacco exposure could lower the threshold of aortic injury necessary for AAA (abdominal aortic aneurysm) development. It has also been used during the isolation of type II pneumocytes from human lungs.
Elastase from porcine pancreas has been used:
  • to induce abdominal aortic aneurysm (AAA)
  • to study the impact of indoleamine 2-3 dioxygenase 1 (IDO) in mice
  • to digest aortas for aortic smooth muscle cells (SMC) isolation
  • as a positive control of proteolytic digestion

Ações bioquímicas/fisiológicas

Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9 kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester. The pH optimum is found to be 8.0-8.5. It does not require any activator, but it is inhibited by diisopropyl fluorophosphate, phenylmethanesulfonyl fluoride, α2-macroglobulin, α1-antitrypsin, sulfonyl fluorides and p-dinitrophenyl diethylphosphate and high salt concentrations. It is extensively used in tissue and cell dissociation procedures. Elastase is effective in the isolation of Type II lung cells.
Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.

Embalagem

Package size based on protein content

Definição da unidade

One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.

Nota de preparo

2× crystallized

Pictogramas

Exclamation markHealth hazard

Palavra indicadora

Danger

Classificações de perigo

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órgãos-alvo

Respiratory system

Código de classe de armazenamento

11 - Combustible Solids

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificado de análise

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Certificado de origem

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Mais documentos

Quotes and Ordering

Demetrios Moris et al.
Current vascular pharmacology, 16(2), 168-178 (2017-02-06)
Abdominal aortic aneurysm (AAA) formation is associated with by inflammation and matrix degradation. This study tested the hypothesis that calprotectin, a novel biomarker for inflammation, as well as established biomarkers such as C-reactive protein (CRP) and matrix metalloproteinase- 9 (MMP-9)
Andreas Giraud et al.
Cardiovascular research, 113(11), 1364-1375 (2017-06-06)
Abdominal aortic aneurysm (AAA), frequently diagnosed in old patients, is characterized by chronic inflammation, vascular cell apoptosis and metalloproteinase-mediated extracellular matrix destruction. Despite improvement in the understanding of the pathophysiology of aortic aneurysm, no pharmacological treatment is yet available to
A C Cunningham et al.
Journal of cell science, 107 ( Pt 2), 443-449 (1994-02-01)
Highly purified populations of alveolar epithelial cells (type II pneumocytes) were isolated from human lung specimens. These cells were characterised histochemically, by demonstrating the presence of intracellular alkaline phosphatase, and morphologically, by electron microscopic demonstration of lamellar bodies and microvilli.
Michel P Bergoeing et al.
Journal of vascular surgery, 45(6), 1217-1227 (2007-04-03)
The development of abdominal aortic aneurysms (AAA) is presumed to result from multiple genetic and environmental factors, with exposure to tobacco smoke the single largest known factor predisposing to aneurysm growth. We have attempted to adapt the elastase-perfused animal model
Liren Duan et al.
Frontiers in physiology, 11, 866-866 (2020-08-09)
Aim: Smoking is a major risk factor for abdominal aortic aneurysm (AAA). Among the components of smoke, nicotine is known to exert pro-atherosclerotic, prothrombotic, and proangiogenic effects on vascular smooth muscle cells (VSMCs). The current study was designed to investigate

Protocolos

Enzymatic Assay of Elastase (EC 3.4.21.36)

Enzymatic Assay of Elastase (EC 3.4.21.36), a continuous spectrophotometric rate determination.

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