E2257

β-Estradiol

powder, γ-irradiated, suitable for cell culture

• Synonym(s): 1,3,5-Estratriene-3,17β-diol, 17β-Estradiol, 3,17β-Dihydroxy-1,3,5(10)-estratriene, Dihydrofolliculin
• Empirical Formula (Hill Notation): C₁₈H₂₄O₂
• CAS Number: 50-28-2
• Molecular Weight: 272.38
• Beilstein: 1914275
• Número EC: 200-023-8
• Número MDL: MFCD00003693
• ID de substância PubChem: 24894462

Properties

• fonte biológica: synthetic (organic)
• Nível de qualidade: 200
• esterilidade: γ-irradiated
• linha de produto: BioXtra
• teor: ≥98% (HPLC)
• forma: powder
• technique(s): cell culture | mammalian: suitable
• pf: 176-180 °C (lit.)
• solubilidade: ethanol: 1 mg/mL, clear, colorless to very faintly yellow
• adequação: suitable for
• enviado em: ambient
• temperatura de armazenamento: room temp
• SMILES string: O[C@H]1CC[C@@]2([H])[C@]3([H])CCC4=CC(O)=CC=C4[C@@]3([H])CC[C@@]21C
• InChI: 1S/C18H24O2/c1-18-9-8-14-13-5-3-12(19)10-11(13)2-4-15(14)16(18)6-7-17(18)20/h3,5,10,14-17,19-20H,2,4,6-9H2,1H3/t14-,15-,16+,17+,18+/m1/s1
• InChI key: VOXZDWNPVJITMN-ZBRFXRBCSA-N
• Gene Information: human ... ESR1(2099) , ESR2(2100) , ESRRA(2101) , ESRRB(2103) , GPER(2852) , SERPINA6(866) ; mouse ... Esr1(13982) , Esr2(13983) , Esrra(26379) ; rat ... Afp(24177) , Ar(24208) , Esr1(24890) , Esr2(25149) , Shbg(24775)

Description

Descrição geral

β-estradiol is a natural human estrogen. It is important for the growth of breast and reproductive epithelia, maturation of long bones and the development of secondary sexual characteristics.[1] Estradiol is produced by immature germ cells, spermatozoa, Leydig cells and Sertoli cells. It plays a crucial role in proliferation, regulation of ion transport and regulation of apoptosis in Sertoli cells. Estradiol regulates sperm concentration, motility and morphology.[2] β-estradiol functions as a menopausal hormone. It is implicated in replacement therapy for female hypogonadism or primary ovarian failure. Reduced levels of β-estradiol are associated with heart disease and osteoporosis.[1]

Aplicação

β-Estradiol is used to study cell differentiation and transformations (tumorigenicity).

β-estradiol has been used:

• for primary culture of mouse endometrial stromal cells (ESCs)[3]
• for estrogen treatment in vitro[4]
• in the in vitro maturation of cumulus–oocyte complexes (COCs)[5]

Ações bioquímicas/fisiológicas

The major estrogen secreted by the premenopausal ovary. Estrogens direct the development of the female phenotype in embryogenesis and during puberty by regulating gene transcription and, thus, protein synthesis. It also induces the production of gonadotropins which, in turn, induce ovulation. Exposure to estradiol increases breast cancer incidence and proliferation.

forma física

powder-RT; stock-frozen in working aliquots, avoid repeated freeze/thaw

Reconstituição

To prepare 20 μg/ml stock solution: add 1 ml absolute ethanol; gently swirl to dissolve; add 49 ml sterile medium while mixing.

Safety Information

• Pictogramas: GHS08,GHS09
• Palavra indicadora: Danger
• Frases de perigo: H351 - H360FD - H362 - H410
• Declarações de precaução: P202 - P260 - P263 - P264 - P273 - P308 + P313
• Classificações de perigo: Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Lact. - Repr. 1A
• Código de classe de armazenamento: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Ponto de fulgor (ºF): Not applicable
• Ponto de fulgor (ºC): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type P3 (EN 143) respirator cartridges