F4799

3X FLAG^® Peptide

lyophilized powder

• Synonym(s): Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, ddddk peptide, dykddddk peptide
• Empirical Formula (Hill Notation): C₁₂₀H₁₆₉N₃₁O₄₉S
• Molecular Weight: 2861.87
• Número MDL: MFCD01863911
• NACRES: NA.32

Properties

• forma: lyophilized powder
• Nível de qualidade: 200
• concentração: (Recommended working concentration is 100 μg/ml for elute 3X FLAG fusion proteins from the ANTI-FLAG^® M2 affinity gel.)
• enviado em: wet ice
• temperatura de armazenamento: 2-8°C

Description

Descrição geral

The 3X FLAG Peptide is a synthetic peptide of 23 amino acid residue. The Asp-Tyr-Lys-Xaa-Xaa-Asp motif is repeated three times in the peptide. Eight amino acids at the C-terminus make up the classic FLAG sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys).

Aplicação

For use in competitive elution of 3X FLAG^® fusion proteins from the ANTI-FLAG^® M2 monoclonal antibody (F1804) in solution or bound to agarose on the ANTI-FLAG^® M2 agarose affinity gel (A2220). This is achieved by Affinity Chromatography. For the gentle elution of 3X FLAG fusion proteins from the ANTI-FLAG^® M2 affinity gels. 1X FLAG Peptide (F3290) will not elute 3X FLAG fusion proteins from ANTI-FLAG^® affinity gels.


Browse additional application references in our FLAG^® Literature portal.

Nota de preparo

To prepare a stock solution, dissolve in TBS (50 mMTris-HCl, pH 7.4, with 150 mM NaCl) at a concentration of 5 mg/ml.

Informações legais

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Safety Information

• Código de classe de armazenamento: 13 - Non Combustible Solids
• WGK: WGK 3
• Ponto de fulgor (ºF): Not applicable
• Ponto de fulgor (ºC): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)