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F6428

Sigma-Aldrich

Reagente de glicerol livre

used for quantitative enzymatic determination of glycerol

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NACRES:
NA.25

forma

solid

uso

40 mL sufficient for 50 reactions

composição

Triton X 100, ≥30-50%

cor

white to off-white

grupo funcional

hydroxyl

doença(s) relevante(s)

obesity

temperatura de armazenamento

2-8°C

SMILES string

C(C(CO)O)O

InChI

1S/C3H8O3/c4-1-3(6)2-5/h3-6H,1-2H2

InChI key

PEDCQBHIVMGVHV-UHFFFAOYSA-N

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Este Item
G77937218126001K
Free Glycerol Reagent used for quantitative enzymatic determination of glycerol

Sigma-Aldrich

F6428

Free Glycerol Reagent

Sigma-Aldrich

Sigma-Aldrich

G7793

Glycerol Standard Solution

293-Free Transfection Reagent Animal-free polycationic liposomal transfection reagent optimized for the transfection of HEK293 cells grown in suspension culture.

Sigma-Aldrich

72181

293-Free Transfection Reagent

Sigma-Aldrich

Sigma-Aldrich

26001K

MAPTrix Reagent

composition

Triton X 100, ≥30-50%

composition

-

composition

-

composition

-

color

white to off-white

color

-

color

-

color

-

functional group

hydroxyl

functional group

-

functional group

-

functional group

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

Descrição geral

The Free Glycerol Reagent provides a precise method for measuring free endogenous glycerol levels through coupled enzyme reactions, bypassing the need for initial lipase hydrolysis. Unlike triglycerides, which are typically bound to proteins and transported in plasma, free glycerol circulates independently. The innovative approach involves enzymatic or alkaline hydrolysis of triglycerides, liberating glycerol and free fatty acids for accurate measurement.

The Serum Triglyceride Determination Kit (Catalog Number TR0100) offers a comprehensive solution for assessing glycerol, true triglycerides, or total triglycerides in serum or plasma samples. Utilizing lipase enzymatic hydrolysis, triglycerides are converted into glycerol and free fatty acids, enabling precise quantification through coupled enzyme reactions by F6428.

Unlike many commercially available triglyceride reagents, this method distinguishes between endogenous glycerol and glycerol derived from lipase hydrolysis of glycerides. With this advanced technology, researchers can confidently analyze glycerol levels with exceptional accuracy and reliability.

Aplicação

The Free Glycerol Reagent facilitates the quantitative enzymatic determination of glycerol levels in serum or plasma. Additionally, it may also be used to measure glycerol release from lipolysis in adipose tissue.

Ações bioquímicas/fisiológicas

Glycerol undergoes phosphorylation through the enzymatic action of glycerol kinase (GK), utilizing adenosine-5′-triphosphate (ATP) to produce glycerol-1-phosphate (G-1-P) and adenosine-5′-diphosphate (ADP). Subsequently, glycerol-1-phosphate (G-1-P) is oxidized by glycerol phosphate oxidase (GPO) to yield dihydroxyacetone phosphate (DAP) and hydrogen peroxide (H2O2). This process involves peroxidase (POD), which catalyzes the coupling of hydrogen peroxide (H2O2) with 4-aminoantipyrine (4-AAP) and sodium N-ethyl-N-(3-sulfopropyl) m-anisidine (ESPA) to generate a quinoneimine dye exhibiting an absorbance maximum at 540 nm.The observed increase in absorbance at 540 nm correlates directly with the concentration of free glycerol present in the sample. This method provides a reliable means for quantifying free glycerol levels, enabling accurate assessment of biochemical samples.

Características e benefícios

  • Simplified Process: Streamline your workflow with a single reagent, offering a quick 5-minute reaction time.
  • User-Friendly Design: Eliminate the need for weighing or mixing multiple reagents, ensuring ease of use and efficiency.

Princípio

O reagente glicerol livre usa reações enzimáticas acopladas, resultando em um aumento na absorbância em 540 nm que é diretamente proporcional à concentração de glicerol.

Ligação

Além dos kits, os reagentes individuais e o padrão de glicerol estão disponíveis separadamente, quando menos reações são necessárias.

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

dust mask type N95 (US), Eyeshields, Gloves


Certificados de análise (COA)

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Os números dos produtos são combinados com os tamanhos/quantidades da embalagem quando exibidos no site (exemplo: T1503-25G). Por favor, certifique-se você insere APENAS o número do produto no campo Número do produto (exemplo: T1503).

Exemplo

T1503
Número do produto
-
25G
Tamanho/quantidade da embalagem

Exemplos adicionais:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

inserir como 1.000309185)

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  • Se encontrar um número de lote como TO09019TO - insira o número do lote 09019TO sem as duas primeiras letras.

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  • Se encontrar um número de lote com um código de preenchimento como STBB0728K9 - digite o número do lote STBB0728 sem o código de preenchimento K9.

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Claire Regazzetti et al.
Diabetes, 58(1), 95-103 (2008-11-06)
Obesity is characterized by an overgrowth of adipose tissue that leads to the formation of hypoxic areas within this tissue. We investigated whether this phenomenon could be responsible for insulin resistance by studying the effect of hypoxia on the insulin
Floriana Rotondo et al.
PeerJ, 4, e2725-e2725 (2016-12-06)
White adipose tissue (WAT) is a complex, diffuse, multifunctional organ which contains adipocytes, and a large proportion of fat, but also other cell types, active in defense, regeneration and signalling functions. Studies with adipocytes often require their isolation from WAT
GIP receptor deletion in mice confers resistance to high-fat diet-induced obesity via alterations in energy expenditure and adipose tissue lipid metabolism
Boer GA, et al.
American Journal of Physiology. Endocrinology and Metabolism, 320, E835-E845 (2021)
Geonho Kim et al.
Journal of insect physiology, 106(Pt 1), 13-19 (2017-05-20)
Acetic acid is a fermentation product of many microorganisms, including some that inhabit the food and guts of Drosophila. Here, we investigated the effect of dietary acetic acid on oviposition and larval performance of Drosophila. At all concentrations tested (0.34-3.4%)
Adipocyte Gs but not Gi signaling regulates whole-body glucose homeostasis
Caron A, et al.
Molecular Metabolism, 27, 11-21 (2019)

Artigos

Lipid Induced Insulin Resistance

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