hemoglobin, ≤25 mg/dL
not available in USA, Canada
cell culture | mammalian: suitable
≤10 EU/mL endotoxin
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10 - Combustible liquids
Insira o número de lote para pesquisar o Certificado de análise (COA).
Insira o número de lote para pesquisar o Certificado de origem (COO).
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
Not all Fetal Bovine Serum products are heat inactivated. Product F9665 is the heat-inactivated version of F7524.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
No, Product No. F7524 is only available in Europe.
Product is South American Origin and EU approved. Some batches could be sourced from France.
This is a non-USDA approved serum. The serum is tested for BVD (bovine virus diarrhea), PI3 (parainfluenza type 3), and IBR (infectious bovine rhinotracheitis) only.
This product is sterilized by filtration through three (3) sterile filter membranes, each of which has a pore size of 0.1 microns.
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
At this time, Sigma-Aldrich does not offer delipidated bovine serum (either FBS or calf serum).A method for lipid depletion, though not necessarily that of Sigma:1. While mixing, gradually add 10 g fumed silica (Product S5130 or Aerosil) per liter of serum2. Mix at room temperature and then transfer to 2-8 °C for overnight mixing.3. After overnight mixing, remove flumed silica (Aerosil) by centrifugation at 3000-5000 rpm for 5 minutes.4. Retain the supernatant. The lipids should stick to the silica. There may be some protein loss
Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored refrigerated at 2-8°C. If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days. For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance. For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C. For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.
Ask a Scientist here.
Cultured cells are excellent hosts for the propagation of many types of viruses. The ability of cell culture systems to produce large quantities of attenuated viral particles has served as the basis for the production of both human and veterinary vaccines. Traditional methods have relied on the production of viral agents in cells cultured in medium supplemented with serum, most commonly fetal bovine serum (FBS).
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