The reconstituted gelatin solution is recommended to be sterilized by autoclaving at 121 °C, 15 psi for 30 minutes.
G1890
Porcine Gelatin
from porcine skin, Type A, powder, gel strength ~300 g Bloom, suitable for electrophoresis and cell culture
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100 G
R$ 318,00
500 G
R$ 1.042,00
1 KG
R$ 1.718,00
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Estamos aqui para ajudarNome do produto
Gelatina, powder, gel strength ~300 g Bloom, Type A, BioReagent, suitable for electrophoresis, suitable for cell culture
fonte biológica
Porcine skin
Nível de qualidade
esterilidade
sterile
tipo
Type A
linha de produto
BioReagent
Formulário
powder
peso molecular
50—100 kDa
embalagem
pkg of 1 kg
pkg of 100 g
pkg of 500 g
concentração
70—90% Biuret
técnica(s)
cell culture | mammalian: suitable
electrophoresis: suitable
cobertura de superfície
100‑200 μg/cm2
solubilidade
H2O: soluble 50 mg/mL, clear to hazy, faintly yellow
Condições de expedição
ambient
temperatura de armazenamento
room temp
Procurando produtos similares? Visita Guia de comparação de produtos
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Este Item | G9391 | G6144 | G2625 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable, electrophoresis: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| sterility sterile | sterility sterile | sterility - | sterility - |
| biological source Porcine skin | biological source bovine skin | biological source - | biological source Porcine skin |
| concentration 70—90% Biuret | concentration - | concentration - | concentration 65-85% (biuret) |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form powder | form powder | form - | form powder |
Descrição geral
Gelatin from porcine skin is generated from the acidic digestion of collagen and is referred as type A. Porcine gelatin comprises majorly glycine, proline and hydroxyproline.[1] Gelatin takes up random coil structure after digestion from the triple helical collagen.[2] It differs from type B bovine gelatin at the N-terminal sequence.[3]
Aplicação
Gelatin has been used in many applications. It has been used in coating cell culture to improve attachment of cells,[4][5] being added to PCR to stabilize Taq DNA, as a blocking reagent in western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has been used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications.[6] In the pharmaceutical industry, gelatin can be used as a suspending and encapsulating agent,[7] among other applications. This product is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.
Características e benefícios
- Sterile gelatin sourced from porcine skin.
- Free from proteases.
- In the form of gelatin powder for convenient and easy handling.
- Versatile for use in cell culture and electrophoresis techniques.
- Provides adequate surface coverage for various applications.
Nota de preparo
This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gel strength of approximately 300.
Outras notas
A gelatina é uma mistura heterogênea de proteínas solúveis em água com massas moleculares médias altas, presente no colágeno. As proteínas são extraídas fervendo a pele, tendões, ligamentos, ossos etc. relevantes em água. A gelatina tipo A é derivada de tecido curado com ácido. A tipo B é derivado de tecido curado com limão.
Exoneração de responsabilidade
Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
nwg
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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Graziano Martello et al.
The EMBO journal, 32(19), 2561-2574 (2013-08-15)
Self-renewal of pluripotent mouse embryonic stem (ES) cells is sustained by the cytokine leukaemia inhibitory factor (LIF) acting through the transcription factor Stat3. Several targets of Stat3 have previously been identified, most notably the reprogramming factor Klf4. However, such factors
Małgorzata Kozikowska et al.
Scientific reports, 9(1), 11969-11969 (2019-08-21)
The retention of the embryo in the transfer catheter after embryo transfer (ET) during in vitro fertilization is a very common phenomenon, encountered by even the most experienced operators, and embryos retained in the transfer catheter or its sleeve require
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Human Embryonic Stem Cell Protocols, 77(1), 317-331 (2009)
Thermosensitive chitosan-gelatin-glycerol phosphate hydrogel as a controlled release system of ferulic acid for nucleus pulposus regeneration
Cheng YH, et al.
Biomaterials, 32(29), 6953-6961 (2011)
The effects of GDF-5 and uniaxial strain on mesenchymal stem cells in 3-D culture
Farng E, et al.
Clinical Orthopaedics and Related Research, 466(8), 1930-1937 (2008)
Artigos
Discussion of synthetic modifications to gelatin, improving the three-dimensional (3D) print resolution, and resulting material properties.
The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.
Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.
The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.
Protocolos
This gelatin coating protocol for cell culture applications offers information regarding types of gelatin, surface coverage concentration, and tips for optimization.
Conteúdo relacionado
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Hi, what is the appropriate way to sterlize a 0.1% gelatin solution, filtration through a 0.22 μm or to autoclave it? Thanks in advance.
1 answer-
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Hello, could you please provide the phenylalanine content (mmol/g) and the number of reactive groups for your porcine gelatin, product G1890? Thank you!
1 answer-
The phenylalanine content in this product has not been determined. According to published literature, Type A Gelatin contains 78-80 millimoles of free carboxyl groups per 100 g of protein.
Helpful?
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How can I store a 0.2% gelatin solution in water (filtered through a 0.22 μm filter) for an extended period, and what are the suitable storage temperature and period?
1 answer-
Gelatin-coated plates can be stored at 2 - 8°C for up to 4 weeks provided they are sealed well to prevent contamination and/or over-drying up of gelatin.
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What is lysine amino acid composition of this gelain?
1 answer-
The amino acid profile is not determined for this product on a lot-to-lot basis. However, historical data indicates a Lysine range of 4.1 - 5.2 % by weight.
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What is the purity of this polymer?
1 answer-
The protein content of this material ranges from 70 - 90%. This information is lot specific and reported in the Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/g1890#product-documentation.Helpful?
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how many amine groups does Type A gelatin has
1 answer-
The number of amine groups in G1890 is not determined.
Helpful?
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What is the molecular weight of this Gelatin?
What is the molecular weight of this Gelatin?
1 answer-
The molecular weight of this product has not been evaluated, However, the molecular weight is closely related to the Bloom Number. The estimated ranges are as follows:
Bloom Number Average Molecular Mass (Da)
50−125 (Low Bloom) 20,000−25,000
175−225 (Medium Bloom) 40,000−50,000
225−325 (High Bloom) 50,000−100,000Please see the link below to review the general datasheet for Gelatin:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/625/g9382pis.pdfHelpful?
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COULD YOU PERHAPS PROVIDE THE NUMBER OF CARBOXYLIC ACID GROUPS IN MOL PER 100 G OF PROTEIN
1 answer-
According to published literature, Type A Gelatin has 78-80 millimoles of free carboxyl groups per 100 g of protein. Please see the link below to review the full product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/187/614/g6650-et-al-pis-ms.pdfHelpful?
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What is the molecular weight of gelatin from porcine skin, Type A?
1 answer-
The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin gelatin vary from 90 to 300 g. This product has a gel strength of approximately 300 g. Thus, the average molecular mass would be 50,000-100,000. Please review the Product Information Sheet for further information:
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