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Merck

G2625

Gelatina

gel strength ~175 g Bloom, Type A

Sinônimo(s):

Teleostean gelatine

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100 G

R$ 529,00

500 G

R$ 1.142,00

1 KG

R$ 1.670,00

R$ 529,00

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Sobre este item

Número CAS:
9000-70-8
MDL number:
MFCD00460869
EC Number:
232-554-6
NACRES:
NA.61
UNSPSC Code:
12352202
Form:
powder
Biological source:
Porcine skin

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biological source

Porcine skin

type

Type A

form

powder

concentration

65-85% (biuret)

technique(s)

cell culture | mammalian: suitable

solubility

H2O: soluble 50 mg/mL

Quality Level

200

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Este Item
G6144G250048722
Gelatin from porcine skin gel strength ~175 g Bloom, Type A

Sigma-Aldrich

G2625

Gelatin from porcine skin

Gelatin from porcine skin gel strength 80-120 g Bloom, Type A

Sigma-Aldrich

G6144

Gelatin from porcine skin

Gelatin from porcine skin gel strength 300, Type A

Sigma-Aldrich

G2500

Gelatin from porcine skin

Gelatin from porcine skin medium gel strength, suitable for microbiology

Millipore

48722

Gelatin from porcine skin

biological source

Porcine skin

biological source

-

biological source

Porcine skin

biological source

Porcine

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

concentration

65-85% (biuret)

concentration

-

concentration

-

concentration

-

form

powder

form

-

form

powder

form

powder

solubility

H2O: soluble 50 mg/mL

solubility

-

solubility

H2O: soluble 50 mg/mL

solubility

H2O: 67 mg/mL at 50 °C, slightly hazy, slightly yellow

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Other Notes

A gelatina é uma mistura heterogênea de proteínas solúveis em água com massas moleculares médias altas, presente no colágeno. As proteínas são extraídas fervendo a pele, tendões, ligamentos, ossos etc. relevantes em água. A gelatina tipo A é derivada de tecido curado com ácido. A tipo B é derivado de tecido curado com limão.

Application

This product is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.

Gelatin has been used in many applications. It has use in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, geltan can be used as a suspending and encapsulating agent, among other applications.

Disclaimer

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Preparation Note

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gel strength of 175.

Classe de armazenamento

11 - Combustible Solids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análise (COA)

Lot/Batch Number
0000526692
0000526691
0000526690
0000526690
0000526691

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Questions

1–2 of 2 Questions  

  1. What is the molecular weight of this Gelatin?

    What is the molecular weight of this Gelatin?

    1 answer

    1. The molecular weight of this product has not been evaluated, However, the molecular weight is closely related to the Bloom Number. The estimated ranges are as follows:
      Bloom Number Average Molecular Mass (Da)
      50−125 (Low Bloom) 20,000−25,000
      175−225 (Medium Bloom) 40,000−50,000
      225−325 (High Bloom) 50,000−100,000

      Please see the link below to review the general datasheet for Gelatin:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/625/g9382pis.pdf

      Helpful?

  2. What is the molecular weight of this Gelatin?

    1 answer

    1. The molecular weight of this product has not been evaluated, However, the molecular weight is closely related to the Bloom Number. The estimated ranges are as follows:
      Bloom Number Average Molecular Mass (Da)
      50−125 (Low Bloom) 20,000−25,000
      175−225 (Medium Bloom) 40,000−50,000
      225−325 (High Bloom) 50,000−100,000

      Please see the link below to review the general datasheet for Gelatin:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/625/g9382pis.pdf

      Helpful?

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