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G5154

Sigma-Aldrich

Glasgow Minimum Essential Medium

With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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Sinônimo(s):
BHK medium, GMEM
Número MDL:
NACRES:
NA.75

esterilidade

sterile-filtered

forma

liquid

technique(s)

cell culture | mammalian: suitable

Impurezas

endotoxin, tested

componentes

L-glutamine: no
phenol red: 0.0213 g/L
NaHCO3: 2.75 g/L
glucose: 4.5 g/L (Dextro)

enviado em

ambient

temperatura de armazenamento

2-8°C

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Este Item
G6148G2549M0450
Sigma-Aldrich

Sigma-Aldrich

G5154

Glasgow Minimum Essential Medium

Sigma-Aldrich

Sigma-Aldrich

G6148

Glasgow Minimum Essential Medium

SAFC

SAFC

G2549

Glasgow Minimum Essential Medium

Sigma-Aldrich

Sigma-Aldrich

M0450

Minimum Essential Medium Eagle

form

liquid

form

powder

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

impurities

-

impurities

Endotoxin, tested

impurities

Endotoxin, tested

components

L-glutamine: no
phenol red: 0.0213 g/L
NaHCO3: 2.75 g/L
glucose: 4.5 g/L (Dextro)

components

glucose: 4.5 g/L
phenol red: 0.0213 g/L
L-glutamine: 0.292 g/L
NaHCO3: no

components

phenol red: 0.0213 g/L
L-glutamine: 0.292 g/L
NaHCO3: 2.75 g/L
glucose: 4.5 g/L (Dextro)

components

glucose: 1.0 g/L
L-glutamine: 0.292 g/L
phenol red: 0.011 g/L
NaHCO3: 2.2 g/L

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

Descrição geral

For use with adherent kidney cell lines such as baby hamster kidney cells (BHK).
Glasgow Minimum Essential Medium has been developed by modifying the Eagle′s BME medium. This medium can be used to study the genetic factors affecting cell competence.

Aplicação

Glasgow Minimum Essential Medium has been used to culture:
  • Hoxb7- green fluorescence protein (GFP) mouse embryonic stem (ES) cell line for the induction of ureteric bud differentiation
  • murine E14 Tg2a embryonic stem (mES) cells
  • transduced Chinese hamster ovary (CHO) cells for the expression and production of Siglec-1-Fc fusion proteins

Reconstituição

Supplement with 0.292 g/L L-glutamine and Tryptose phosphate broth solution (T 8159) at 100 ml/L of medium.

Código de classe de armazenamento

12 - Non Combustible Liquids

WGK

WGK 1

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable


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Os números dos produtos são combinados com os tamanhos/quantidades da embalagem quando exibidos no site (exemplo: T1503-25G). Por favor, certifique-se você insere APENAS o número do produto no campo Número do produto (exemplo: T1503).

Exemplo

T1503
Número do produto
-
25G
Tamanho/quantidade da embalagem

Exemplos adicionais:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

inserir como 1.000309185)

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Tryptose Phosphate Broth suitable for insect cell culture

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L-Glutamine solution 200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture

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Marco D'Addio et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35(11), e22017-e22017 (2021-10-27)
Cellular interactions between endothelial cells and macrophages regulate macrophage localization and phenotype, but the mechanisms underlying these interactions are poorly understood. Here we explored the role of sialoglycans on lymphatic endothelial cells (LEC) in interactions with macrophage-expressed Siglec-1 (CD169). Lectin-binding
Marieke Aarts et al.
Genes & development, 31(20), 2085-2098 (2017-11-16)
Expression of the transcription factors OCT4, SOX2, KLF4, and cMYC (OSKM) reprograms somatic cells into induced pluripotent stem cells (iPSCs). Reprogramming is a slow and inefficient process, suggesting the presence of safeguarding mechanisms that counteract cell fate conversion. One such
Cinzia Caprio et al.
Disease models & mechanisms (2021-02-21)
The Ezh2 gene encodes a histone methyltransferase of the Polycomb Repressive Complex 2 that methylates histone H3 lysine 27. In this work we asked whether EZH2 has a role in the development of the pharyngeal apparatus and whether it regulates
Edward C Hsiao et al.
PloS one, 3(7), e2532-e2532 (2008-07-04)
Fluorescent reporters are useful for assaying gene expression in living cells and for identifying and isolating pure cell populations from heterogeneous cultures, including embryonic stem (ES) cells. Multiple fluorophores and genetic selection markers exist; however, a system for creating reporter
Isabelle Re Nett et al.
EMBO reports, 19(8) (2018-06-14)
Mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) signalling is implicated in initiation of embryonic stem (ES) cell differentiation. The pathway is subject to complex feedback regulation. Here, we examined the ERK-responsive phosphoproteome in ES cells and identified the negative regulator

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