G5422

β-Glycerophosphate disodium salt hydrate

≤1.0 mol % L-α-isomer

• Synonym(s): BGP, Glycerol 2-phosphate disodium salt hydrate
• Linear Formula: (HOCH₂)₂CHOP(O)(ONa)₂ · xH₂O
• CAS Number: 154804-51-0
• Molecular Weight: 216.04 (anhydrous basis)
• Número EC: 212-464-3
• Número MDL: MFCD00149082
• ID de substância PubChem: 329799941
• NACRES: NA.25

Properties

• fonte biológica: synthetic
• Nível de qualidade: 300
• forma: powder
• Impurezas: ≤0.1% Inorganic phosphorus; ≤1.0 mol % L-α-isomer
• pf: 102-104 °C (lit.)
• solubilidade: water: 50 mg/mL, clear, colorless
• SMILES string: O.[Na+].[Na+].OCC(CO)OP([O-])([O-])=O
• InChI: 1S/C3H9O6P.2Na.H2O/c4-1-3(2-5)9-10(6,7)8;;;/h3-5H,1-2H2,(H2,6,7,8);;;1H2/q;2*+1;/p-2
• InChI key: ROPZSVKNEIIIDE-UHFFFAOYSA-L

Description

Aplicação

β-Glycerophosphate is a classical serine-threonine phosphatase inhibitor used in kinase reaction buffers. BGP is often used in combination with other phosphatase/protease inhibitors for broad spectrum inhibition. It functions as an organic phosphate donor and has been used in culture media for mesenchymal stem cell differentiation to osteoblast-type cells. BGP is also used to buffer M17 media for Lactococcus culture in recombinant protein expression.

β-glycerophosphate is used in homegenization solutions for muscles extracts following fixation.

Initiates differentiation of bone-marrow-derived stem cells embedded in a natural collagen/chitosan gel designed for tissue engineering.

Qualidade

Low inorganic phosphate content. Mixtures of α- and β-glycerophosphate have historically been used in microbiological media but high levels of inorganic (free) phosphate has been associated with the irregular growth of bacteria and unwanted precipitates.

Safety Information

• Código de classe de armazenamento: 13 - Non Combustible Solids
• WGK: WGK 3
• Ponto de fulgor (ºF): Not applicable
• Ponto de fulgor (ºC): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)