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G5893

Sigma-Aldrich

Gamborg′s B-5 Basal Medium with Minimal Organics

fine powder, suitable for plant cell culture

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Código UNSPSC:
12352207
NACRES:
NA.72

forma

fine powder

Nível de qualidade

200

técnica(s)

cell culture | plant: suitable

aplicação(ões)

agriculture

temperatura de armazenamento

2-8°C

Categorias relacionadas

Descrição geral

Gamborg′s B-5 basal medium is an efficient nutrient mixture for plants.
Gamborg′ B-5 medium may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg′s B-5 (B5) medium for in vitro culture of plant cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis. Gamborg′s B-5 medium has been used for the culture of Annona squamosa callus.

Aplicação

Gamborg′s B-5 Basal Medium with Minimal Organics has also been used:
  • as a component of liquid media for the tissue culture of Brassica rapa
  • as a component of callus inducing medium for culturing Arabidopsis cardiolipin mutant seedlings
  • as a component of solid GM medium for germination of Arabidopsis seeds

Variante da fórmula

With the macro- and micronutrients, and vitamins as described by Gamborg, et al. (1968).

Media Formulation

Quantidade

Formulated to contain 3.2 grams of powder per liter of medium.

Pictogramas

Flame over circle
GHS03

Palavra indicadora

Warning

Frases de perigo

H272

Declarações de precaução

P210 - P220 - P280 - P370 + P378 - P501

Classificações de perigo

Ox. Sol. 3

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

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Experiments in Plant Tissue Culture (1985)
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In eukaryotes the presence of the dimeric phospholipid cardiolipin (CL) is limited to the mitochondrial membranes. It resides predominantly in the inner membrane where it interacts with components of the mitochondrial electron transfer chain. CL deficiency has previously been shown
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Classical Plant Media Formulations

Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.

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