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G8168

Sigma-Aldrich

G 418 disulfate salt solution

50 mg/mL in H2O, 0.1 μm filtered, BioReagent, suitable for cell culture

Sinônimo(s):
Antibiotic G418
Empirical Formula (Hill Notation):
C20H40N4O10 · 2H2SO4
Número CAS:
Peso molecular:
692.71
Número MDL:
ID de substância PubChem:
NACRES:
NA.76

Nível de qualidade

200

fonte biológica

Micromonospora echinospora

esterilidade

0.1 μm filtered

linha de produto

BioReagent

forma

solution

potência

50-60 mg per mL

peso molecular

692.7

embalagem

pkg of 10 and 100 mL

concentração

50 mg/mL in H2O

technique(s)

cell culture | mammalian: suitable

Impurezas

endotoxin, tested

cor

colorless

solubilidade

H2O: 50 mg/mL, clear

espectro de atividade do antibiótico

parasites

Modo de ação

protein synthesis | interferes

temperatura de armazenamento

2-8°C

SMILES string

OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O

InChI

1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1

InChI key

UHEPSJJJMTWUCP-NKCAIAFTSA-N

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Categorias relacionadas

Descrição geral

Chemical structure: aminoglycoside

Aplicação

G418 disulfate is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL.

Ações bioquímicas/fisiológicas

Mode of Action: G418 blocks polypeptide synthesis and protein elongation by inhibiting synthesis at the 70S and 80S ribosomes.

Antimicrobial Spectrum: G418 selects for cells stably transfected with an iNOS promoter construct and neomycin resistance gene and shows activity against protozoa and helminthes.

Atenção

The product is stable for two years when stored at 2-8°C.

Pictogramas

Health hazard

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Resp. Sens. 1 - Skin Sens. 1

Código de classe de armazenamento

12 - Non Combustible Liquids

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Certificado de análise

Certificado de origem

Joan Lin-Cereghino et al.
Yeast (Chichester, England), 25(4), 293-299 (2008-03-11)
The methylotrophic yeast, Pichia pastoris, is widely used as a host organism for the expression of heterologous proteins. Currently, the Zeocin and blasticidin resistance genes are the only dominant selectable markers that can be used for primary selection of transformants.
Jieru Li et al.
Nature structural & molecular biology, 27(11), 1032-1040 (2020-09-23)
Transcription activation by distal enhancers is essential for cell-fate specification and maintenance of cellular identities. How long-range gene regulation is physically achieved, especially within complex regulatory landscapes of non-binary enhancer-promoter configurations, remains elusive. Recent nanoscopy advances have quantitatively linked promoter
Lene Halkjær et al.
Frontiers in immunology, 11, 774-774 (2020-05-21)
We aimed at establishing a sensitive and robust assay for estimation of systemic complement activation at complement component C3 level in mouse and human plasma samples. In order to capture the activation products iC3b and C3dg in a specific and
Kelvin Kai-Wan Hui et al.
Journal of cellular and molecular medicine, 23(3), 1784-1797 (2018-12-15)
Aberrant regulation of programmed cell death (PCD) has been tied to an array of human pathologies ranging from cancers to autoimmune disorders to diverse forms of neurodegeneration. Pharmacologic modulation of PCD signalling is therefore of central interest to a number
Frida C Mohlin et al.
Molecular immunology, 65(2), 367-376 (2015-03-04)
Atypical hemolytic uremic syndrome (aHUS) is a disease of complement dysregulation, characterized by hemolytic anemia, thrombocytopenia and acute renal failure. Mutations in complement inhibitors are major risk factors for development of aHUS. The three aHUS patients reported in this study

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