GE17-5280-02

nProtein A Sepharose® 4 Fast Flow

Cytiva 17-5280-02, pack of 200 mL

NACRES:
NA.56

prazo de validade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

embalagem

pack of 200 mL

manufacturer/tradename

Cytiva 17-5280-02

condição de armazenamento

(20% Ehtanol)

matriz

4% cross-linked agarose

diâmetro médio

90 μm (d50v)

limpeza

2 - 10 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)

faixa de trabalho

3 - 9

capacidade

>30 mg binding capacity(human IgG/ml)

adequação

suitable for bioprocess medium

temperatura de armazenamento

2-8°C

Categorias relacionadas

Descrição geral

nProtein A Sepharose® 4 Fast Flow is native protein A coupled to Sepharose® 4 Fast Flow for the purification of monoclonal and polyclonal antibodies at both laboratory and process scale.

nProtein A Sepharose® 4 Fast Flow is native protein A coupled to the well established Sepharose® 4 Fast Flow base matrix. The native protein A ligand is produced by fermenting a selected strain of Staphylococcus aureus. The purified protein is coupled to the cross-linked 4% agarose base matrix by the cyanogen bromide technique, giving a highly stable medium with minimal non-specific adsorption. nProtein A Sepharose® 4 Fast Flow is manufactured without using animal-derived components.

nProtein A Sepharose® 4 Fast Flow has nearly twice the total IgG binding capacity of Protein A Sepharose® CL-4B, and is suitable for recovery and purification of monoclonal antibodies from cell culture at both laboratory and process scale. nProtein A Sepharose® 4 Fast Flow was developed and tested in co-operation with leading manufacturers of purified monoclonal antibody products, and is used in routine commercial production.

As member of the BioProcess media range, nProtein A Sepharose® 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Características e benefícios

  • Replaces Protein A Sepharose® 4 Fast Flow, the first Cytiva Protein A medium for large-scale purification of antibodies.
  • Used in routine commercial production of monoclonal antibodies
  • Free from animal-derived components.

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

Sepharose is a registered trademark of Cytiva

RIDADR

NONH for all modes of transport

Certificado de análise
Certificado de origem
Artigos
This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.
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This page describes immunoprecipitation (immunoaffinity or pull-down techniques).
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This page describes efficient column packing and preparation for affinity chromatography of antibodies.
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Protocolos
This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.
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Protein A is derived from a strain of Staphylococcus aureus and contains five regions that bind to the Fc region of IgG. As an affinity ligand, protein A is coupled to Sepharose so that these regions are free to bind IgG.
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This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from Cytiva.
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This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from Cytiva.
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