Hot Start Mix RTG is a premixed, predispensed, ambient temperature-
stable formulation that can effectively reduce nonspecific priming and primer-dimer formation during PCR. The system is based on a novel hot start method that uses a hot start activator protein to sequester primers prior to PCR, thereby making them unavailable for nonspecific priming during the reaction preparation. This method requires only a quick 2 min initial PCR activation step. In contrast, other commercially available hot start PCR kits that are based on chemical inactivation require prolonged incubation at high temperatures, which may damage the DNA template by heat-induced depurination. The net result from using Hot Start Mix RTG is a more robust and reliable amplification of the target amplicons with increased yield when compared with conventional hot start master mix products. Furthermore, the mix does not contain Taq antibody and this eliminates the risk of mammalian-source contamination.
The advantages of the Ready-To-Go (RTG) format over other hot start mixes include increased reproducibility, long-term storage at room temperature, and a more convenient reaction setup. You can expect consistent and reliable PCR results with Hot Start Mix RTG because we have minimized pipetting steps and reduced the potential for errors and contamination.
The only additional reagents required are water, primers, and template DNA. Additional MgCl2 can be easily added, allowing users to customize this reagent to their specific needs. The beads are provided predispensed into either 0.2-ml or 0.5-ml PCR tubes that are compatible with most thermal cyclers. The 0.2 ml tubes come assembled in a 96-well (8 × 12) plate format that allows individual strips of eight tubes to be easily removed. This flexibility allows use of the entire 96-well plate, strips of eight, or individual 0.2 ml tubes.
This Ready-To-Go format provides reproducible and reliable performance for demanding PCR applications in which high specificity and high sensitivity are essential to success.