If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
H0887
HEPES
1M, liquid, pH 7.0-7.6, suitable for cell culture, BioXtra, BioReagent
Sinônimo(s):
Ácido N-(2-hidroxietil)piperazina-N′-2-etanossulfônico
Selecione um tamanho
| Tamanho/SKU | Disponibilidade | Preço |
|---|
Sobre este item
Nome do produto
HEPES, 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture
sterility
sterile-filtered
Quality Segment
product line
BioReagent, BioXtra
assay
0.9—1.1 M
form
liquid
storage condition
(Tightly closed. Dry), dry at room temperature
concentration
1 M
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
color
colorless
pH
7.0-7.6
useful pH range
6.8-8.2
7.5
density
1.1 g/cm3
suitability
suitable for component for culture media, suitable for electrophoresis
application(s)
advanced drug delivery
cell analysis
life science and biopharma
sample preparation
storage temp.
2-8°C
SMILES string
OCCN1CCN(CCS(=O)(O)=O)CC1
InChI
1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key
JKMHFZQWWAIEOD-UHFFFAOYSA-N
General description
Além disso, supera os tampões de bicarbonato (NaHCO3) em sua capacidade de manter o pH fisiológico, apesar das flutuações na concentração de dióxido de carbono decorrentes da respiração celular, um fator fundamental na manutenção da homeostase celular. Além de seu excepcional desempenho na cultura celular, o HEPES também pode ser aplicado em vários estudos biológicos e bioquímicos. Também pode ser valioso em técnicas como imunoprecipitação, lise celular e imagens de células vivas, proporcionando um ambiente estável e controlado para procedimentos experimentais. A combinação de sua versatilidade, capacidade excepcional de tamponamento de pH e interação mínima com outras moléculas posiciona o HEPES como uma ferramenta indispensável em diversos campos de pesquisa.
Application
- solução de sal balanceada de Hank, meio de Eagle modificado de Dulbecco e DMEM sem glicose, que são usados para a preparação de cortes de tecido
- Tampões usados em medições de condutância localizadas no lado basolateral das inserções de para examinar o meio de cultura de células de seletividade de cátions
- meios de cultura celular para fornecer capacidade de tampão para manter o pH da linhagem celular
- tampão na descelularização do núcleo pulposo porcino.
Features and Benefits
- Produto de alta pureza para pesquisa bioquímica e biológica
- Filtrado estéril e testado para cultura de células
- A solução pronta reduz a necessidade
- Útil para a faixa de variação de pH de 6,8-8,2 e pKa de 7,5 a 25 ºC
- A solução disponível pronta reduz a necessidade de tempo de preparo
Preparation Note
Other Notes
Disclaimer
1 of 1
Este Item | |||
|---|---|---|---|
| description 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture | description BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered | description BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered | description BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration) |
| assay 0.9—1.1 M | assay - | assay - | assay ≥99.5% (titration) |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - |
| concentration 1 M | concentration 1 M | concentration 1 M | concentration - |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 400 |
| product line BioXtra, BioReagent | product line - | product line - | product line BioXtra |
| form liquid | form liquid | form liquid | form powder |
| assay 0.9—1.1 M | assay - | assay - | assay ≥99.5% (titration) |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - |
| concentration 1 M | concentration 1 M | concentration 1 M | concentration - |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 400 |
| product line BioXtra, BioReagent | product line - | product line - | product line BioXtra |
| form liquid | form liquid | form liquid | form powder |
Still not finding the right product?
Explore all of our products under HEPES
Classe de armazenamento
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
Escolha uma das versões mais recentes:
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
-
How can I determine the shelf life / expiration / retest date of this product?
1 answer-
Helpful?
-
-
Hello! Is it dissolved in H2O entirely? or does it have other surfactants?
1 answer-
This product is comprised of HEPES free acid in tissue culture grade water. No other additives or preservatives are present, including surfactants.
Helpful?
-
-
How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
-
-
Bonjour, Est-ce que ce produit H0887 peut-être congelé à -80°C sans risque de le faire précipiter ?
1 answer-
It is best not to freeze the product as received. However, should the product be inadvertently frozen, it is best to thaw the product slowly. Do not place the frozen product in an incubator or water bath. Once thawed, inspect for any precipitates before use. A clear, colorless solution will be suitable for further use. Should this buffer be required to prepare the stock solution of another reagent that requires freezer storage, thaw slowly if possible.
Helpful?
-