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H0887

Sigma-Aldrich

HEPES

1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture

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Sinônimo(s):
Ácido N-(2-hidroxietil)piperazina-N′-2-etanossulfônico
Número CAS:
Número MDL:
ID de substância PubChem:
NACRES:
NA.75

esterilidade

sterile-filtered

Nível de qualidade

linha de produto

BioReagent
BioXtra

forma

liquid

condição de armazenamento

(Tightly closed. Dry)

concentração

1 M

technique(s)

cell culture | mammalian: suitable

Impurezas

endotoxin, tested

pH

7.0-7.6

useful pH range

6.8-8.2

temperatura de armazenamento

2-8°C

SMILES string

OCCN1CCN(CCS(=O)(O)=O)CC1

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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Este Item
H3537H3537H6147
HEPES solution 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture

Sigma-Aldrich

H0887

HEPES solution

Essential+ Grade
HEPES solution BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

Sigma-Aldrich

H3537

HEPES solution

Premium Grade
HEPES solution BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

Sigma-Aldrich

H3537

HEPES solution

Premium Grade
HEPES BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)

Sigma-Aldrich

H6147

HEPES

Premium Grade
product line

BioReagent, BioXtra

product line

-

product line

-

product line

BioXtra

form

liquid

form

liquid

form

liquid

form

powder

storage condition

(Tightly closed. Dry)

storage condition

-

storage condition

-

storage condition

-

concentration

1 M

concentration

1 M

concentration

1 M

concentration

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | embryo: suitable

sterility

sterile-filtered

sterility

0.2 μm filtered

sterility

0.2 μm filtered

sterility

-

product line

BioReagent, BioXtra

product line

-

product line

-

product line

BioXtra

form

liquid

form

liquid

form

liquid

form

powder

storage condition

(Tightly closed. Dry)

storage condition

-

storage condition

-

storage condition

-

concentration

1 M

concentration

1 M

concentration

1 M

concentration

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | embryo: suitable

Descrição geral

O HEPES foi descrito como um dos melhores tampões multiuso disponíveis para pesquisa biológica. Em pH biológico, a molécula é zwitteriônica, e é eficaz como tampão em pH 6,8 a 8,2 (pKa de 7,55). Geralmente é usado em culturas de células a concentrações entre 5 mM e 30 mM. O HEPES foi usado em uma ampla gama de aplicações, inclusive cultura de tecidos. Ele é comummente usado para tamponar meios de cultura celular em ar. O HEPES é utilizado em experimentos in vitro em Mg.
HEPES Buffer Solution, a sterile-filtered 1M solution of 4-(2-hydroxyethyl)piperazine-1-ethane-sulfonic acid, serves as a vital biological buffer in cell biology, biochemical, and biological research. This zwitterionic buffer enjoys widespread adoption due to its effective pH range of 6.8 to 8.2, making it suitable for a diverse array of research applications. HEPES excels as a buffering agent in cell culture media, offering several advantages over alternatives like Tris and phosphate. Notably, HEPES exhibits lower cell toxicity, ensuring optimal cell health and viability during experiments.

Furthermore, it surpasses bicarbonate buffers (NaHCO3) in its ability to maintain physiological pH despite fluctuations in carbon dioxide concentration arising from cellular respiration, a key factor in maintaining cellular homeostasis. Beyond its exceptional performance in cell culture, HEPES may also find application in various biological and biochemical studies. It may also be valuable in techniques like immunoprecipitation, cell lysis, and live cell imaging, providing a stable and controlled environment for experimental procedures. The combination of its versatility, exceptional pH buffering capacity, and minimal interaction with other molecules positions HEPES as an indispensable tool across diverse research fields.

Aplicação

O HEPES foi usado como componente de:
  • Solução salina balanceada de Hank, meio de Eagle modificado por Dulbecco (DMEM) e DMEM sem glicose, que são usados para preparar cortes de tecidos
  • Tampões usados em medições localizadas de condutância no lado basolateral de insertos de cultura para examinar a seletividade de cátions

Características e benefícios

  • High purity product for biochemical and biological research
  • Sterile filtered and cell culture tested
  • Ready Solution reduces the need
  • Useful for the pH range of 6.8 - 8.2 and pKa of 7.5 at 25 °C
  • Ready available solution reduce the need for preparation time

Atenção

Concentrações de HEPES acima de 40 mM podem ser tóxicas para alguns tipos de células.

Nota de preparo

Preparado a partir de ácido livre de HEPES e água para cultura celular.

Código de classe de armazenamento

10 - Combustible liquids

WGK

WGK 1

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable


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1000309185

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HEPES, Free Acid, Molecular Biology Grade A zwitterionice N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.48 at 25°C.

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L-Glutamine solution 200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture

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Kirkland Nt
Acta Biomaterialia, 8, 925- 936 (2012)
The role of the endocrine system in feeding-induced tissue-specific circadian entrainment.
Sato M, et al.
Cell Reports, 8(2), 393-401 (2014)
Kaixi Tang et al.
PLoS computational biology, 14(7), e1006303-e1006303 (2018-07-06)
Focal adhesions are protein complexes that anchor cells to the extracellular matrix. During migration, the growth and disassembly of these structures are spatiotemporally regulated, with new adhesions forming at the leading edge of the cell and mature adhesions disassembling at
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