Merck
All Photos(1)

H0887

Sigma-Aldrich

HEPES solution

1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture

Synonym(s):
N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
CAS Number:
Número MDL:
ID de substância PubChem:
NACRES:
NA.75

Nível de qualidade

300

esterilidade

sterile-filtered

linha de produto

BioReagent
BioXtra

forma

liquid

concentração

1 M

technique(s)

cell culture | mammalian: suitable

Impurezas

endotoxin, tested

pH

7.0-7.6

useful pH range

6.8-8.2

temperatura de armazenamento

2-8°C

SMILES string

OCCN1CCN(CCS(=O)(O)=O)CC1

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Descrição geral

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Aplicação

HEPES has been used as a component of:
  • Hank′s balanced salt solution, Dulbecco′s modified eagle′s medium and no-glucose DMEM, which are used for the preparation of tissue slices
  • Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity

Atenção

Concentrations of HEPES over 40mM may be toxic to some cell types.

Nota de preparo

Prepared from HEPES free acid and cell culture grade water.

Código de classe de armazenamento

10 - Combustible liquids

WGK

WGK 1

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service