Merck
Todas as fotos(1)

H3537

Sigma-Aldrich

HEPES

BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

Faça loginto View Organizational & Contract Pricing

Select a Size

Sinônimo(s):
Ácido N-(2-hidroxietil)piperazina-N′-2-etanossulfônico
Número CAS:
Número MDL:
ID de substância PubChem:
NACRES:
NA.25

grau

BioPerformance Certified

Nível de qualidade

esterilidade

0.2 μm filtered

forma

liquid

concentração

1 M

technique(s)

cell culture | mammalian: suitable

Impurezas

Bioburden, tested
DNase, RNase, Protease, Nickase, free
endotoxin, tested

pH

5.0-6.0

useful pH range

6.8-8.2

traços de cátion

Fe: <5 ppm
heavy metals (as Pb): <5 ppm

adequação

suitable for molecular biology

application(s)

diagnostic assay manufacturing

SMILES string

OCCN1CCN(CCS(=O)(O)=O)CC1

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

Procurando produtos similares? Visit Guia de comparação de produtos

Comparar Itens Semelhantes

Ver Comparação Total

Mostrar diferenças

1 of 4

Este Item
H0887H0887H3375
HEPES solution BioPerformance Certified, 1&#160;M, suitable for cell culture, 0.2 &#956;m filtered

Sigma-Aldrich

H3537

HEPES solution

Premium Grade
HEPES solution 1&#160;M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture

Sigma-Aldrich

H0887

HEPES solution

Premium Grade
HEPES solution 1&#160;M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture

Sigma-Aldrich

H0887

HEPES solution

Premium Grade
HEPES &#8805;99.5% (titration)

Sigma-Aldrich

H3375

HEPES

-
sterility

0.2 μm filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

form

liquid

form

liquid

form

liquid

form

crystalline powder

concentration

1 M

concentration

1 M

concentration

1 M

concentration

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

Bioburden, tested, DNase, RNase, Protease, Nickase, free, endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

-

grade

BioPerformance Certified

grade

-

grade

-

grade

-

sterility

0.2 μm filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

form

liquid

form

liquid

form

liquid

form

crystalline powder

concentration

1 M

concentration

1 M

concentration

1 M

concentration

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

Bioburden, tested, DNase, RNase, Protease, Nickase, free, endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

-

Descrição geral

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Aplicação

RNAse HEPES has been used:
  • To supplement Dulbecco′smodified Eagle′smedium for maintenance of cell line and RPMI medium to wash rat islets
  • To recover purified ribonucleotide
  • As a component of HEPES/KOH buffer and adenylation buffer for small RNA isolation and sequencing
  • As a component in buffers used for nuclear extract preparation and also to supplement RPMI-1640 medium for maintenance of islets

Código de classe de armazenamento

10 - Combustible liquids

WGK

WGK 1

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Os números dos produtos são combinados com os tamanhos/quantidades da embalagem quando exibidos no site (exemplo: T1503-25G). Por favor, certifique-se você insere APENAS o número do produto no campo Número do produto (exemplo: T1503).

Exemplo

T1503
Número do produto
-
25G
Tamanho/quantidade da embalagem

Exemplos adicionais:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

inserir como 1.000309185)

Está com problemas? Sinta-se à vontade para entrar em contato Assistência Técnica para obter assistência

Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Produtos Aldrich

  • Se encontrar um número de lote como TO09019TO - insira o número do lote 09019TO sem as duas primeiras letras.

  • Se encontrar um número de lote com um código de preenchimento, como 05427ES-021 - insira o número do lote 05427ES sem o código de preenchimento -021.

  • Se encontrar um número de lote com um código de preenchimento como STBB0728K9 - digite o número do lote STBB0728 sem o código de preenchimento K9.

Não encontra o que está procurando?

Em alguns casos, um COA pode não estar disponível on-line. Se a sua pesquisa não encontrou o COA, você pode solicitar um.

Solicitar COA

Os clientes também visualizaram

Slide 1 of 7

1 of 7

HEPES

SAFC

PHG0001

HEPES

HEPES ULTROL&#174; Grade, &#8805;99% dry basis (titration), 1M zwitterionic N-substituted aminosulfonic acid buffer

Millipore

375368

HEPES

HEPES BioXtra, pH 5.0-6.5 (1&#160;M in H2O), &#8805;99.5% (titration)

Sigma-Aldrich

H7523

HEPES

HEPES potassium salt &#8805;99.5% (titration)

Sigma-Aldrich

H0527

HEPES potassium salt

HEPES BioXtra, suitable for mouse embryo cell culture, &#8805;99.5% (titration)

Sigma-Aldrich

H6147

HEPES

Sodium chloride solution 5&#160;M in H2O, BioReagent, for molecular biology, suitable for cell culture

Sigma-Aldrich

S5150

Sodium chloride solution

Expression of an uncleavable N-terminal RasGAP fragment in insulin-secreting cells increases their resistance toward apoptotic stimuli without affecting their glucose-induced insulin secretion.
Yang JY, et al.
The Journal of Biological Chemistry, 280(38), 32835-32842 (2005)
Timing of CRISPR/Cas9-related mRNA microinjection after activation as an important factor affecting genome editing efficiency in porcine oocytes.
Sato M, et al.
Theriogenology, 108, 29-38 (2018)
A Small RNA Isolation and Sequencing Protocol and Its Application to Assay CRISPR RNA Biogenesis in Bacteria.
Silas S, et al.
Bio-protocol, 8(4) (2018)
Masahiro Sato et al.
International journal of molecular sciences, 16(8), 17838-17856 (2015-08-08)
Some reports demonstrated successful genome editing in pigs by one-step zygote microinjection of mRNA of CRISPR/Cas9-related components. Given the relatively long gestation periods and the high cost of housing, the establishment of a single blastocyst-based assay for rapid optimization of
Masahiro Sato et al.
Theriogenology, 108, 29-38 (2017-12-02)
Recently, successful one-step genome editing by microinjection of CRISPR/Cas9-related mRNA components into the porcine zygote has been described. Given the relatively long gestational period and the high cost of housing swine, the establishment of an effective microinjection-based porcine genome editing

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica