This product is not tested for immunoglobulins such as IgG . Currently, there is no human serum product that is tested specifically for IgG. The testing performed can be found on the lot specific Certificate of Analysis:
https://www.sigmaaldrich.com/product/sigma/h3667#product-documentation
Please see the link below to review product S1-100ML. This product is tested for Total Globulins.
https://www.sigmaaldrich.com/product/mm/s1m
H3667
Soro humano
Heat Inactivated, from human male AB plasma, USA origin, sterile-filtered
Human Serum
Sinônimo(s):
plasma humano, soro humano, soros humanos
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| Para você/SKU | Disponibilidade | Preço |
|---|---|---|
20 mL | Verificar disponibilidade no carrinho | R$ 1.164,00 |
100 mL | Disponível para envio HOJEdeCajamar | R$ 3.755,00 |
Sobre este item
Biological source:
human male plasma (Type AB)
Origin:
USA origin
Sterility:
sterile-filtered
Shipped in:
dry ice
R$ 1.164,00
Verificar disponibilidade no carrinho
Assistência Técnica
Precisa de ajuda? Nossa equipe de cientistas experientes está à sua disposição.
Estamos aqui para ajudarbiological source
human male plasma (Type AB)
Quality Segment
sterility
sterile-filtered
composition
hemoglobin, ≤20 mg/dL
origin
USA origin
technique(s)
cell culture | mammalian: suitable
impurities
≤10 EU/mL endotoxin
shipped in
dry ice
storage temp.
−20°C
General description
O soro é necessário principalmente como suplemento em meios de cultura celular. Ele contém macromoléculas, proteínas transportadoras, fatores de fixação e espalhamento, nutrientes de baixo peso molecular e hormônios e fatores de crescimento.
Application
O soro humano foi usado como suplemento em meios de cultura para monócitos.[1] Ele foi usado como controle em western blotting para estudar a expressão de flightless I no soro.
Packaging
A embalagem pode variar em lotes diferentes devido à escassez de frascos usados para embalar esse produto. A presença ou ausência de envoltório retrátil ao redor da tampa do frasco não’afeta a integridade/esterilidade do produto.
Analysis Note
Testado quanto à presença de endotoxinas
Other Notes
Não é incomum que os produtos de soro humano sejam turvos. Este produto é proveniente de soro agregado, e a turbidez indica que existem lipídios presentes no soro. Não existem produtos de soro humano livres de lipídios disponíveis.
Disclaimer
SOMENTE PARA USO EM PESQUISA. Esse produto é regulamentado na França quando se destina ao uso para fins científicos, inclusive para atividades de importação e exportação (Artigo L 1211-1 parágrafo 2 do Código de Saúde Pública). O comprador (ou seja, o usuário final) deve obter uma autorização de importação do Ministério da Pesquisa da França, conforme o Artigo L1245-5-1 II. do Código de Saúde Pública. Ao solicitar este produto, você confirma que obteve a autorização de importação adequada.
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Este Item | |||
|---|---|---|---|
| origin USA origin | origin USA origin | origin USA origin | origin USA origin |
| biological source human male plasma (Type AB) | biological source human male plasma (Type AB) | biological source human | biological source human |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable, cell culture | stem cell: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable, cell culture | stem cell: suitable |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin | impurities (mycoplasma tested, virus tested), ≤10 EU/mL endotoxin | impurities ≤10 EU/mL Endotoxin |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
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Classe de armazenamento
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Is the IgG concentration measured ?
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Is the only difference between H3667 and H4522 that H3667 is heat inactivated whilst H4522 is not? And what makes a big difference in the availability between them?
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Yes, the difference between product H3667 and H4522, is that product H3667 is heat inactivated. Any availability questions can be directed to customer service. Please navigate to the link below:https://www.sigmaaldrich.com/support/customer-support.
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Is this tested for Anti HLA antibodies? I am thinking of it’s application as a flow cytometric negative control
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This product has not been tested for the presence of anti-HLA antibodies.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Why is serum Heat-Inactivated (HI)?
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Heat inactivation of serum is performed to inactivate complement (which can cause cell lysis or downstream problems in immunoassays). If there are immunoglobulins present in the serum that can bind to the cells in culture (either specifically or non-specifically), there is a chance for antibody-mediated complement cytotoxicity (ADCC, causing cell death). It may also reduce the titer of some mycoplasma and some viruses. Often, heat-inactivated serum is used because of the adoption of a previous protocol. It is mainly used when culturing hematopoetic cells or performing immunological type assays such as mixed-lymphocyte reactions or neutralization assays.FBS may not be heat inactivated as this is not required for all applications. It limits the loss of the other protein activity that may be minimally present in the serum.A protocol for heat inactivation:1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Sigma-Aldrich does not recommend repeated thaw-freeze cycles.
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What is the difference between human serum from AB plasma and human serum from AB clotted whole blood?
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Product H6914 is prepared from whole blood that is allowed to clot, centrifuged and the serum removed. There is no additional processing required.Product H4522 is prepared from anticoagulated (sodium citrate) whole blood. This is centrifuged to pellet the cells (RBC, WBC, some platelets) and the plasma is removed. This plasma is then treated with calcium to activate the clotting cascade. The preparation is centrifuged again and the supernatant is removed. This product can also be called plasma derived serum.Product H6914 will contain more growth factors since it is allowed to spontaneously clot and there will be the release of growth factors from many of the cells (WBC, platelets) present. The cells are removed before the plasma is clotted for Product H4522, so this plasma will have fewer growth factors present (more similar to what is circulating). Unless testing for growth factors, most researchers are looking for Product H6914.We offer both products heat inactivated.Product H5667 Human Serum Heat Inactivated, from male AB clotted whole bloodProduct H3667 Human Serum Heat Inactivated, from human male AB plasma
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