Merck
Todas as fotos(3)

H6147

Sigma-Aldrich

HEPES

BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)

Sinônimo(s):
N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
Empirical Formula (Hill Notation):
C8H18N2O4S
Número CAS:
Peso molecular:
238.30
Beilstein:
883043
Número EC:
Número MDL:
ID de substância PubChem:
NACRES:
NA.75

Nível de qualidade

400

linha de produto

BioXtra

teor

≥99.5% (titration)

forma

powder

technique(s)

cell culture | embryo: suitable

pH

5.0-6.5 (25 °C, 238 g/L)

useful pH range

6.8-8.2

pKa (25 °C)

7.5
7.5

solubilidade

water: 500 mg/mL, clear, colorless

absorção

≤0.05 at 290 in H2O at 33%

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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Descrição geral

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg. It is typically used in cell culture at concentration between 5mM to 30mM.

Aplicação

HEPES has been used:
  • As a constituent of modified Eagle′s minimum essential medium for in vitro culturing of spermatogenic cells and as a constituent of the cryoprotectant used to preserve these germ cells
  • As a component of modified Ham′s F-10 for the incubation of doe zygotes to carry out Haploid male hemizygous recipient production
  • in the preparation of 90% Percoll solution used in Percoll- gradient method for sperm collection and preparation

Embalagem

100, 500 g in poly bottle

Outras notas

This product has been qualified for use in cell and mouse embryo culture.
Easily compare specifications for HEPES products with the HEPES specification table.

propriedades físicas

Useful pH range: 6.8 - 8.2

Código de classe de armazenamento

11 - Combustible Solids

WGK

WGK 1

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificado de análise

Insira o número de lote para pesquisar o Certificado de análise (COA).

Certificado de origem

Insira o número de lote para pesquisar o Certificado de origem (COO).

Short-term in-vitro culture and cryopreservation of spermatogenic cells used for human in-vitro conception.
Aslam I and Fishel S
Human Reproduction, 13(3), 634-638 (1998)
Lilianna Szyk-Warszyńska et al.
Polymers, 11(5) (2019-05-28)
Multilayer films containing α- and β-casein and polypeptides, poly-L-lysine (PLL), and poly-L-arginine (PLArg) were formed by the layer-by-layer technique and Fourier Transform InfraRed spectroscopy with Attenuated Total Reflection (FTIR-ATR) and FTIR/Grazing Angle analyzed their infrared spectra. We investigated the changes
Viable offspring derived from cryopreserved haploid rabbit parthenotes.
Garci'a-Ximenez F and Escriba MJ
Theriogenology, 57(4), 1319-1325 (2002)
The effects of L-ergothioneine and L-ascorbic acid on the in vitro maturation (IVM) and embryonic development (IVC) of sheep oocytes.
Pancarci SM, et al.
Kafkas U?niversitesi veteriner faku?ltesi dergisi, 16, 757-763 (2010)
Assessing the corrosion of biodegradable magnesium implants: a critical review of current methodologies and their limitations.
Kirkland NT, et al.
Acta Biomaterialia, 8(3), 925-936 (2012)

Artigos

Embryo Culture Media

Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture

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