Merck
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H6648

Sigma-Aldrich

Hanks′ Balanced Salt solution

Modified, with sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):
HBSS
NACRES:
NA.75

Nível de qualidade

400

esterilidade

sterile-filtered

forma

liquid

technique(s)

cell culture | mammalian: suitable

Impurezas

endotoxin, tested

componentes

glucose: 1.0 g/L (Dextro)
phenol red: no
NaHCO3: 0.35 g/L

enviado em

ambient

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Descrição geral

The essential function of Hanks′ Balanced Salt solution is to maintain pH as well as osmotic balance. It also provides water and essential inorganic ions to cells.
Formulation: click here.

Aplicação

Calcium and magnesium support cell adhesion. Used to minimize cell attachment or aggregation.
Hanks′ Balanced Salt solution has been used:

  • to study the degradation and cytotoxic effects of the composite material and the ion and/or magnetite release from the scaffolds.
  • to measure the stability of the magnetic scaffold material in bone tissue engineering.
  • in Fluorometric Measurements of intracellular calcium levels [Ca2+]
  • for isolating polymorphonuclear leukocytes (PMN) from umbilical cord blood
  • For assessing airway inflammation in mice

Outras notas

Phenol red has been shown to interfere with the growth of some cells at cloning densities. Use this medium when growing cells at low densities.

Código de classe de armazenamento

12 - Non Combustible Liquids

WGK

nwg

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Protocols

Rat Dermal Fibroblasts (RDF) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Human Astrocytes (HA) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Human Carotid Artery Endothelial Cells (HCtAEC) Culture Protocol

Technical information for working with human carotid artery endothelial cells including thawing, subculturing and cryopreservation

Rat Brain Microvascular Endothelial Cells (RBMVEC) Culture Protocol

Technical information for working with rat brain microvascular endothelial cells including thawing, subculturing and cryopreservation

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