Merck
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H7523

Sigma-Aldrich

HEPES

BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration)

Sinônimo(s):
N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
Empirical Formula (Hill Notation):
C8H18N2O4S
Número CAS:
Peso molecular:
238.30
Beilstein:
883043
Número EC:
Número MDL:
ID de substância PubChem:

Nível de qualidade

400

linha de produto

BioXtra

teor

≥99.5% (titration)

forma

powder

Impurezas

Insoluble matter, passes filter test

resíduo de ignição (900 °C)

≤0.1% (as SO4)

perda

≤1.0% loss on drying, 110°C

pH

5.0-6.5 (1 M in H2O)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubilidade

H2O: 1 M, clear, colorless

traços de ânion

chloride (Cl-): ≤0.05%

traços de cátion

Al: ≤0.0005%
As: ≤0.0001%
Ba: ≤0.0005%
Bi: ≤0.0005%
Ca: ≤0.001%
Cd: ≤0.0005%
Co: ≤0.0005%
Cr: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Li: ≤0.0005%
Mg: ≤0.0005%
Mn: ≤0.0005%
Mo: ≤0.0005%
Na: ≤0.005%
Ni: ≤0.0005%
Pb: ≤0.0005%
Sr: ≤0.0005%
Zn: ≤0.0005%

absorção

≤0.035 at 280 in H2O at 1 M
≤0.045 at 260 in H2O at 1 M

application(s)

diagnostic assay manufacturing

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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Descrição geral

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Aplicação

HEPES has been used:
  • As a component of homogenisation buffer used in protein sample preparation
  • To neutralise PRP (platelet-rich plasma) solution as part of PRP preparation
  • As a component in 2X PBS buffer, which is used in phosphate calcium transfection in cell line preparation
  • As a supplement in RPMI 1640 medium used to culture human umbilical vein endothelial cells (HUVEC) and in M199 complete medium for ex-vivo treatment of testicular tissue.

Embalagem

50, 250 g in poly bottle

Outras notas

Easily compare specifications for HEPES products with the HEPES specification table.

Código de classe de armazenamento

13 - Non Combustible Solids

WGK

WGK 1

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificado de análise

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Certificado de origem

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The inhibitory effect of intracerebroventricularly injected interleukin 1? on testosterone secretion in the rat: role of steroidogenic acute regulatory protein.
Ogilvie KM, et al.
Biology of Reproduction, 60(2), 527-533 (1999)
Hsp60-induced tolerance in the rotifer Brachionus plicatilis exposed to multiple environmental contaminants.
Wheelock CE, et al.
Archives of Environmental Contamination and Toxicology, 36(3), 281-287 (1999)
Intervertebral disc regeneration in an ex vivo culture system using mesenchymal stem cells and platelet-rich plasma.
Chen WH, et al.
Biomaterials, 30(29), 5523-5533 (2009)
In vitro tubulogenesis of endothelial cells by relaxation of the coupling extracellular matrix-cytoskeleton.
Deroanne CF, et al.
Cardiovascular Research, 49(3), 647-658 (2001)
Assessing the corrosion of biodegradable magnesium implants: a critical review of current methodologies and their limitations.
Kirkland NT, et al.
Acta Biomaterialia, 8(3), 925-936 (2012)

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