HPA002691

Sigma-Aldrich

Anti-MRE11 antibody produced in rabbit

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

Sinônimo(s):
Anti-Double-strand break repair protein MRE11A antibody produced in rabbit, Anti-MRE11 homolog 1 antibody produced in rabbit, Anti-MRE11A, Anti-ATLD, Anti-MRE11 meiotic recombination 11 homolog A antibody produced in rabbit
Número MDL:
Número do Atlas de Proteínas Humanas:

fonte biológica

rabbit

Nível de qualidade

100

forma do anticorpo

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

linha de produto

Prestige Antibodies® Powered by Atlas Antibodies

forma

buffered aqueous glycerol solution

species reactivity

rat, human, mouse

embalagem

antibody small pack of 25 μL

aplicação(ões)

immunoblotting: 0.04-0.4 μg/mL
immunofluorescence: 0.25-2 μg/mL
immunohistochemistry: 1:200-1:500

sequência de imunogênio

EESASAFSADDLMSIDLAEQMANDSDDSISAATNKGRGRGRGRRGGRGQNSASRGGSQRGRADTGLETSTRSRNSKTAVSASRNMSIIDAFKSTRQQPSRNVTTKNYSEVIEVDESDVEEDIFPTTSKTDQRWSSTSSSK

conjugado

unconjugated

enviado em

wet ice

temperatura de armazenamento

−20°C

Gene Information

human ... MRE11A(4361)

Descrição geral

MRE11A (MRE11 homolog A), a nuclear protein, shows similarity with its yeast counterpart at the amino-terminal half. It is highly expressed in proliferating tissues along with other tissues. It is composed of the nuclease and capping domains. It forms dimer structure through the interfaces in two ways: β3-α3 loop from one MRE11 and loop β4-β5 from another MRE11 as well as loop α2-β3 from one MRE11A and helices α2-α3 from another MRE11.

Imunogênio

MRE11 homolog, double strand break repair nuclease recombinant protein epitope signature tag (PrEST)

Aplicação

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org)and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.

Ações bioquímicas/fisiológicas

MRE11A (MRE11 homolog A) is a DNA repair protein which acts on double strand DNA damage by cleaving broken ends. In addition, it also participates in DNA replication and telomere maintenance. MRE11A exhibits 3′ to 5′ exonuclease activity. MRE11A mutation leads to the ataxia telangiectasia-like disorder (ATLD).

Características e benefícios

Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:
  • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
  • Protein array of 364 human recombinant protein fragments.

Ligação

Corresponding Antigen APREST78243.

forma física

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Informações legais

Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Alemanha

WGK 1

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificado de análise

Certificado de origem

S A Pitts et al.
Human molecular genetics, 10(11), 1155-1162 (2001-05-24)
We showed recently that mutation of the hMRE11 gene identified a new ataxia telangiectasia-like disorder (ATLD). In this report we describe the genomic organization of the hMRE11 gene and the analysis of a promoter region that appears to direct the...
T T Paull et al.
Molecular cell, 1(7), 969-979 (1998-07-04)
MRE11 and RAD50 are known to be required for nonhomologous joining of DNA ends in vivo. We have investigated the enzymatic activities of the purified proteins and found that Mre11 by itself has 3' to 5' exonuclease activity that is...
Shyng-Shiou F Yuan et al.
Journal of the National Cancer Institute, 104(19), 1485-1502 (2012-08-24)
Previous studies have shown that altered forms of MRE11, a protein known to play a vital role in DNA double-strand break repair, DNA replication, and telomere maintenance are associated with cancer outcomes. We investigated the role of MRE11 in breast...
Young Bong Park et al.
Structure (London, England : 1993), 19(11), 1591-1602 (2011-11-15)
Mre11 plays an important role in repairing damaged DNA by cleaving broken ends and by providing a platform for other DNA repair proteins. Various Mre11 mutations have been identified in several types of cancer. We have determined the crystal structure...
J H Petrini et al.
Genomics, 29(1), 80-86 (1995-09-01)
Mutation of the Saccharomyces cerevisiae RAD52 epistasis group gene, MRE11, blocks meiotic recombination, confers profound sensitivity to double-strand break damage, and has a hyperrecombinational phenotype in mitotic cells. We isolated a highly conserved human MRE11 homologue using a two-hybrid screen...

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