This stock solution may be diluted with sterile water, medium, or an aqueous buffer such as HEPES or phosphate-buffered saline. Sterile saline is also acceptable for dilution.
I0516
Insulina
BioReagent, 10 mg/mL insulin in 25 mM HEPES, pH 8.2, sterile-filtered, suitable for cell culture
Sinônimo(s):
Bovine insulin, Cell culture grade insulin
para ver os preços organizacionais e de contrato.
Selecione um tamanho
Alterar visualização
| Para você/SKU | Disponibilidade | Preço |
|---|
Sobre este item
Biological source:
bovine
Form:
solution
Preço e disponibilidade não estão disponíveis no momento.
Assistência Técnica
Precisa de ajuda? Nossa equipe de cientistas experientes está à sua disposição.
Estamos aqui para ajudarbiological source
bovine
Quality Segment
sterility
sterile-filtered
product line
BioReagent
form
solution
concentration
10 mg/mL insulin in 25 mM HEPES, pH 8.2
technique(s)
cell culture | mammalian: suitable, single cell analysis: suitable
impurities
endotoxin, tested
pH
7.9-8.5
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
SMILES string
SC[C@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CN)[C@H](CC)C)C(C)C)CCC(=O)O)CCC(=O)N)CS)C(=O)N[C@@H](C)C(=O)N[C@@H](CO)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CS)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc4ccc(cc4)O)C(=O)N[
InChI
1S/2C97H151N25O34S4/c2*1-12-46(10)77(120-72(131)35-98)96(154)122-76(45(8)9)94(152)107-55(24-28-74(134)135)79(137)104-53(22-26-69(100)128)82(140)116-66(40-159)93(151)118-64(38-157)90(148)103-47(11)78(136)114-63(37-124)89(147)121-75(44(6)7)95(153)119-67(41-160)92(150)115-62(36-123)88(146)109-57(30-43(4)5)84(142)110-58(31-48-13-17-50(125)18-14-48)85(143)105-52(21-25-68(99)127)80(138)108-56(29-42(2)3)83(141)106-54(23-27-73(132)133)81(139)112-60(33-70(101)129)87(145)111-59(32-49-15-19-51(126)20-16-49)86(144)117-65(39-158)91(149)113-61(97(155)156)34-71(102)130/h2*13-20,42-47,52-67,75-77,123-126,157-160H,12,21-41,98H2,1-11H3,(H2,99,127)(H2,100,128)(H2,101,129)(H2,102,130)(H,103,148)(H,104,137)(H,105,143)(H,106,141)(H,107,152)(H,108,138)(H,109,146)(H,110,142)(H,111,145)(H,112,139)(H,113,149)(H,114,136)(H,115,150)(H,116,140)(H,117,144)(H,118,151)(H,119,153)(H,120,131)(H,121,147)(H,122,154)(H,132,133)(H,134,135)(H,155,156)/t2*46-,47-,52-,53-,54-,55-,56-,57-,58-,59-,60-,61-,62-,63-,64-,65-,66-,67-,75-,76-,77-/m00/s1
InChI key
XYPANFGXFLOTDK-CJLNJPJHSA-N
Gene Information
bovine ... INS(280829)
cow ... INS(280829)
Application
Foi utilizada uma solução de insulina do pâncreas bovino:
- para estudar o efeito da insulina exógena sobre as concentrações plasmáticas de glicose, insulina, fator de crescimento semelhante à insulina I (IGF-I) e progesterona (P4) em novilhas de corte púberes[1]
- para estudar a capacidade de resposta à insulina da placenta durante complicações na gravidez usando células trofoblásticas humanas primárias e explantes de vilosidades placentárias[2]
- para estudar o efeito da sinalização do receptor de insulina na densidade de espinhas dendríticas em neurônios do hipocampo de camundongos neonatos in vitro[3]
Biochem/physiol Actions
A insulina é um importante hormônio redutor de glicose, cuja insuficiência causa a hiperglicemia observada no diabetes tipo 2.[4]
Hormônio polipeptídico de duas cadeias produzido pelas células β das ilhotas pancreáticas. Seu peso molecular é de cerca de 5800 Da. As cadeias α e β são ligadas por duas pontes dissulfeto entre as cadeias. A cadeia α contém uma ponte dissulfeto dentro da cadeia. A insulina regula a captação celular, o uso e o armazenamento de glicose, aminoácidos e ácidos graxos, além de inibir a quebra de glicogênio, proteínas e gordura.
Physical form
estável a 2-8 °C
1 of 1
Este Item | |||
|---|---|---|---|
| biological source bovine | biological source - | biological source bovine | biological source - |
| product line BioReagent | product line - | product line BioReagent | product line - |
| technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) liquid chromatography (LC): suitable | technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) - |
| form solution | form liquid | form powder | form - |
| concentration 10 mg/mL insulin in 25 mM HEPES, pH 8.2 | concentration 100 μg/mL (PBS pH 7.2) | concentration - | concentration - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. - |
Classe de armazenamento
12 - Non Combustible Liquids
ppe
Eyeshields, Gloves
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Escolha uma das versões mais recentes:
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
-
Is there a specific solution that I should use to further dilute or is sterile saline ok?
1 answer-
Helpful?
-
-
Can I freeze the aliquots at -20 or -80 for the long-term storage?
1 answer-
The USP recommends that solutions of insulin be stored at 2-8°C. It is not recommended to freeze solutions of insulin, as it can aggregate. Sterile solutions should be stable for at least 1 year at 2-8°C.
Helpful?
-
-
What is the shelf life of this product if stored at 2-8°C ? Thanks.
1 answer-
This product is assigned an expiration date of 2 years from the date of manufacture. The expiration date is lot-specific and is printed on the label, as well as in the Certificate of Analysis. Please navigate to the ‘DOCUMENTATION’ section of the Product Detail Page to access a Certificate under ‘Certificate of Analysis’:
https://www.sigmaaldrich.com/product/sigma/i0516#product-documentationHelpful?
-
-
Is bovine insulin (I0516) suitable for use in rat cell culture?
1 answer-
The product has not been validated for rat cell culture application. However, based on the following citations, it appears to work well with rat cell culture:
•Isolation, Transfection, and Long-Term Culture of Adult Mouse and Rat Cardiomyocytes
published in J Vis Exp. 2020 Oct 10;(164):10.3791/61073. PMID: 33104067.•Collagen hydrogels with controllable combined cues of elasticity and topography to regulate cellular processes" published in Biomed Mater. 2021 Jun 11;16(4). PMID: 34030146.
Helpful?
-
-
Does this solution contain zinc? How would that affect how quickly the insulin would act in a mammalian cell culture?
1 answer-
The product contains 10 mg/mL insulin in 25 mM HEPES, pH 8.2, but it does not include zinc. Currently, the effect of zinc on insulin signaling is not characterized. However, it is noted that zinc appears to be able to enhance insulin-mediated IR tyrosine phosphorylation according to the article Biol Trace Elem Res. 2004 Oct;101(1):19-36. Effect of chromium and zinc on insulin signaling in skeletal muscle cells. PMID: 15516700.
Helpful?
-
-
is this product suitable for 3T3-L1 differentiation?
1 answer-
This product is supplied as a sterile bovine insulin solution at 10 mg/mL and would be suitable for diffentiation assays. This item has not been tested on 3T3-L1 cells, but is part of the differentiation protocol for the WT-1 preadipocyte cell line, product SCC255. See the link below to review this protocol:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/568/334/scc255ds.pdfHelpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
What is the amino acid sequence for insulin?
1 answer-
The sequence and structure of insulin can be found on the product information sheet (under Documents, above).
Helpful?
-
-
How many units/mg am I getting in Product I0516, Insulin solution?
1 answer-
This solution is made using insulin Product No. I5500. The potency specification for the I5500 is not less than 27 USP units/mg. However, we do not test for potency of the final product of this solution. It is possible some activity is lost during the manufacturing process.
Helpful?
-
-
What is the molarity of Product I0516, Insulin solution?
1 answer-
Product No. I0516 is a 10 mg/mL solution of bovine insulin (MW 5733.5). The molarity is 1.7 μmol/mL or 1.7 mM.
Helpful?
-