Merck
All Photos(1)

I1284

Sigma-Aldrich

Isopropyl β-D-thiogalactopyranoside solution

ReadyMade IPTG solution for Blue-white screening

Synonym(s):
IPTG solution
Empirical Formula (Hill Notation):
C9H18O5S
CAS Number:
Molecular Weight:
238.30
Beilstein:
4631
Número MDL:
ID de substância PubChem:
NACRES:
NA.52

grau

for molecular biology

Nível de qualidade

200

descrição

non-ionic

forma

liquid

enviado em

dry ice

temperatura de armazenamento

−20°C

SMILES string

CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O

InChI

1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1

InChI key

BPHPUYQFMNQIOC-NXRLNHOXSA-N

Descrição geral

Isopropyl thiogalactose (IPTG) is a sugar derivative widely used for the induction of the expression of recombinant proteins in E. coli. This expression system requires that the gene of interest is cloned in plasmids containing the Lac promoter. IPTG Ready Made is a 0.2 μm filter sterilized solution of 200 mM IPTG. This reagent remains liquid at its storage temperature (-20 °C), thus avoiding freeze-thaw cycles and providing a user-friendly solution.

Aplicação

Isopropyl β-D-thiogalactopyranoside solution is:
  • used as an inducer for the expression of single domain antibodies(sdAbs) in E coli and bioactive peptides
  • used in its half-life and decay studies
  • suitable for induction of recombinant protein expression in systems controlled by the lac repressor

Ações bioquímicas/fisiológicas

Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for β--galactosidase. The β-galactosidase gene is transcribed and monosaccharides are the product. Since Isopropyl β-D-thiogalactopyranoside cannot be metabolized by E. coli its concentration remains constant. Therefore, it is not a variable in the experiment.

Componentes

IPTG Solution is composed of 200 mM IPTG in molecular biology grade water.

Princípio

Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for β-galactosidase. The β-galactosidase gene is transcribed and monosaccharides are the product.

Código de classe de armazenamento

12 - Non Combustible Liquids

WGK

WGK 2

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service