Merck
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I2644

Sigma-Aldrich

Interleukin-2 human

IL-2, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Sinônimo(s):
T-cell growth factor, Interleukin-2, IL-2, hIL-2
Número MDL:
NACRES:
NA.77

Nível de qualidade

200

fonte biológica

human

recombinante

expressed in E. coli

forma

lyophilized powder

potência

≤700 EC50

qualidade

endotoxin tested

peso molecular

15.5 kDa

embalagem

pkg of 5X10 μg
pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

Impurezas

≤1 EU/μg

nº de adesão UniProt

temperatura de armazenamento

−20°C

Gene Information

human ... IL2(3558)

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Descrição geral

Interleukin-2 (IL-2) is an α-helical cytokine belonging to T-helper cell type 1. It has a molecular weight of 15kDa. The IL2 gene is mapped to human chromosome 4q27. IL-2 is originally called as T cell growth factor.

Aplicação

Interleukin-2 human has been used:
  • in the stimulation of natural killer cells
  • to study its ability to stimulate lymphocyte growth and prevent apoptosis
  • as an antigen in enzyme-linked immunosorbent assay (ELISA), to analyse the human autoantibody reactivities in health and in type 1 diabetes mellitus

Embalagem

10 μg in poly bottle

Ações bioquímicas/fisiológicas

Interleukin-2 (IL-2) is essential for proliferation of natural killer (NK) cells, lymphocytes and macrophages. It plays a key role in the immune system functions such as NK cell-stimulated immune responses. IL-2 also mediates the essential functions of regulatory T cells. It is implicated in autoimmune disease. IL-2 is associated with apoptosis and thus, possesses antitumor response. It mediates CD41+ T-cells differentiation into T-helper cell 1 and 2 effector subsets, preventing T-helper 17 differentiation.
Interleukin-2 (IL-2) is an immunomodulatory factor produced by certain subsets of T lymphocytes. This lympho­kine promotes long term growth of acti­vated T cells and related cell types. IL-2 plays a role in the activation and proliferation of NK cells, induces γ-inter­feron and B cell growth factor secretion, and modulates the expression of the IL-2 receptor.

forma física

Lyophilized, 0.2 μm filtered, no additives.

Nota de análise

The biological activity is measured by the dose-dependent stimulation of the proliferation of murine CTLL-2 cells.

Código de classe de armazenamento

13 - Non Combustible Solids

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificado de análise

Insira o número de lote para pesquisar o Certificado de análise (COA).

Certificado de origem

Insira o número de lote para pesquisar o Certificado de origem (COO).

Quotes and Ordering

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Where can I find information on the specific activity of Product I2644, Interleukin-2 human - recombinant?

    The EC50 has been determined by dose-dependent stimulation of the proliferation of murine CTLL-2 cells.  You can find the lot specific value for the EC50 of this product on the Certificate of Analysis.  Certificates of Analysis can be viewed on our website by entering the lot number on the product detail page (for I2644).

  4. Is Product I2644, Interleukin-2 human - recombinant, endotoxin tested?

    Yes, the endotoxin levels are reported on the Certificate of Analysis.  Certificates of Analysis can be viewed on our website by entering the lot number on the product page (for I2644).

  5. How can I convert the EC50 of interleukin from ng/mL to units/mL when using Product I2644, Interleukin-2 human - recombinant?

    The specific activity in Units/mg = 1 × 106 / ED50 (in ng/ml).

  6. How should I dissolve Product I2644, Interleukin-2 human - recombinant?

    Reconstitute the contents of the vial using 100 mM acetic acid to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers.

  7. How should solutions of Product I2644, Interleukin-2 human - recombinant, be stored?

    Interleukin solutions can be stored at 2-8°C for up to 1 week. For extended storage, freeze at -20°C in working aliquots to avoid freezing and thawing.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Natural killer cells in the synovial fluid of rheumatoid arthritis patients exhibit a CD56bright, CD94bright, CD158negative phenotype
Pridgeon C, et al.
Rheumatology (Basel), 42(7), 870-878 (2003)
Interleukin 2, 37-37 (2012)
Stefania Braidotti et al.
Frontiers in bioscience (Landmark edition), 27(2), 39-39 (2022-03-01)
The T-cell engager antibody blinatumomab (BlincytoT⁢M) represents a promising rescue therapy for relapsed/refractory CD19+ acute lymphoblastic leukemia (B-ALL), although ~20-30% of patients still do not respond to treatment. Blinatumomab creates a tight synapsis between CD3+ T-lymphocytes and leukemic CD19+ B-cells
Loss of immune tolerance to IL-2 in type 1 diabetes
Perol L, et al.
Nature Communications, 7(2), 13027-13027 (2016)
Association of Interleukin-2-330T/G and Interleukin-10-1082A/G Genetic Polymorphisms with B-Cell Non-Hodgkin Lymphoma in a Cohort of Egyptians
Rahman HAA, et al.
Turkish journal of haematology : official journal of Turkish Society of Haematology, 35(2), 99-99 (2018)

Artigos

Interleukin Family (IL)

The Interleukins comprise a disparate group of cytokines and growth factors that are produced by and released from leukocytes. Interleukin-1β (1L-1β) is released primarily from stimulated macrophages and monocytes and plays a key role in inflammatory and immune responses and may induce anti-tumor immunity.

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