No antibodies are used in the purification of this product.
I6634
Insulina
powder, BioReagent, suitable for cell culture
Sinônimo(s):
Bovine insulin, Cell culture grade insulin
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I6634-50MG
R$ 1.297,00
I6634-100MG
R$ 2.664,00
I6634-250MG
R$ 4.387,00
I6634-500MG
R$ 7.703,00
I6634-1G
R$ 13.255,00
Sobre este item
Fórmula empírica (Notação de Hill):
C254H377N65O75S6
Número CAS:
Peso molecular:
5733.49
Número CE:
Número MDL:
Código UNSPSC:
12352209
NACRES:
NA.77
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Estamos aqui para ajudarfonte biológica
bovine
Nível de qualidade
linha de produto
BioReagent
Formulário
powder
potência
≥25 USP units per mg
técnica(s)
cell culture | mammalian: suitable
single cell analysis: suitable
solubilidade
acetic acid: 2 mg/mL (pH: 2-3)
hydrochloric acid: 2 mg/mL (pH: 2-3)
nº de adesão UniProt
Condições de expedição
ambient
temperatura de armazenamento
−20°C
chave InChI
IXIBAKNTJSCKJM-BUBXBXGNSA-N
Informações sobre genes
cow ... INS(280829)
Procurando produtos similares? Visita Guia de comparação de produtos
1 of 4
Este Item | I5500 | I1882 | I4011 |
|---|---|---|---|
| biological source bovine | biological source - | biological source bovine | biological source bovine |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| product line BioReagent | product line - | product line BioXtra | product line - |
| form powder | form powder | form lyophilized powder | form powder |
| technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | hybridoma: suitable |
| solubility acetic acid: 2 mg/mL (pH: 2-3), hydrochloric acid: 2 mg/mL (pH: 2-3) | solubility water: 2 mg/mL, clear, colorless (pH: 2) | solubility acetic acid: 2 mg/mL (pH: 2-3), hydrochloric acid: 2 mg/mL (pH: 2-3) | solubility acidified water, pH 2.0: 2 mg/mL |
Aplicação
A insulina é usada como fator de crescimento em muitos sistemas de cultura de células de mamíferos.
Ações bioquímicas/fisiológicas
Hormônio de duas cadeias polipeptídicas produzido pelas células β das ilhotas pancreáticas. Seu peso molecular é ~5800 Da. As cadeias α e β são ligadas por duas pontes dissulfeto intercadeia. A cadeia α contém uma ponte dissulfeto intracadeia. A insulina regula a captação, utilização e armazenamento celular de glicose, aminoácidos e ácidos graxos e inibe a quebra de glicogênio, proteína e gordura.
forma física
pó: 0 °C; solução estável a 2-8 °C, 1 ano
Nota de preparo
A insulina tem baixa solubilidade em pH neutro. Ela pode ser solubilizada a 2 mg/ml em ácido clorídrico ou acético diluído, pH 2-3.
Outras notas
InChI: 1S/C254H377N65O75S6/c1-29-131(24)205(312-192(334)103-256)250(389)317-204(130(22)23)246(385)287-158(74-81-199(344)345)216(355)281-155(70-77-188(260)330)220(359)307-183-116-399-400-117-184-242(381)305-177(110-321)238(377)293-161(87-122(6)7)223(362)294-167(94-139-52-60-145(324)61-53-139)226(365)282-153(68-75-186(258)328)217(356)289-160(86-121(4)5)221(360)284-157(73-80-198(342)343)219(358)301-173(100-189(261)331)233(372)297-169(96-141-56-64-147(326)65-57-141)229(368)308-182(241(380)303-175(253(393)394)102-191(263)333)115-398-396-113-180(212(351)270-106-193(335)277-152(71-78-196(338)339)215(354)280-150(50-41-83-268-254(264)265)210(349)269-107-194(336)278-165(92-137-45-35-31-36-46-137)225(364)296-166(93-138-47-37-32-38-48-138)228(367)298-170(97-142-58-66-148(327)67-59-142)236(375)318-206(135(28)323)251(390)319-84-42-51-185(319)244(383)285-151(49-39-40-82-255)213(352)276-134(27)252(391)392)310-248(387)202(128(18)19)315-234(373)163(89-124(10)11)291-227(366)168(95-140-54-62-146(325)63-55-140)295-222(361)159(85-120(2)3)288-207(346)132(25)274-214(353)156(72-79-197(340)341)286-245(384)201(127(16)17)314-235(374)164(90-125(12)13)292-231(370)172(99-144-105-267-119-273-144)300-237(376)176(109-320)279-195(337)108-271-211(350)179(112-395-397-114-181(309-243(183)382)240(379)275-133(26)208(347)304-178(111-322)239(378)316-203(129(20)21)249(388)311-184)306-224(363)162(88-123(8)9)290-230(369)171(98-143-104-266-118-272-143)299-218(357)154(69-76-187(259)329)283-232(371)174(101-190(262)332)302-247(386)200(126(14)15)313-209(348)149(257)91-136-43-33-30-34-44-136/h30-38,43-48,52-67,104-105,118-135,149-185,200-206,320-327H,29,39-42,49-51,68-103,106-117,255-257H2,1-28H3,(H2,258,328)(H2,259,329)(H2,260,330)(H2,261,331)(H2,262,332)(H2,263,333)(H,266,272)(H,267,273)(H,269,349)(H,270,351)(H,271,350)(H,274,353)(H,275,379)(H,276,352)(H,277,335)(H,278,336)(H,279,337)(H,280,354)(H,281,355)(H,282,365)(H,283,371)(H,284,360)(H,285,383)(H,286,384)(H,287,385)(H,288,346)(H,289,356)(H,290,369)(H,291,366)(H,292,370)(H,293,377)(H,294,362)(H,295,361)(H,296,364)(H,297,372)(H,298,367)(H,299,357)(H,300,376)(H,301,358)(H,302,386)(H,303,380)(H,304,347)(H,305,381)(H,306,363)(H,307,359)(H,308,368)(H,309,382)(H,310,387)(H,311,388)(H,312,334)(H,313,348)(H,314,374)(H,315,373)(H,316,378)(H,317,389)(H,318,375)(H,338,339)(H,340,341)(H,342,343)(H,344,345)(H,391,392)(H,393,394)(H4,264,265,268)/t131-,132-,133-,134-,135+,149-,150-,151-,152-,153-,154-,155-,156-,157-,158-,159-,160-,161-,162-,163-,164-,165-,166-,167-,168-,169-,170-,171-,172-,173-,174-,175-,176-,177-,178-,179-,180-,181-,182-,183-,184-,185-,200-,201-,202-,203-,204-,205-,206-/m0/s1
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Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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Down-regulation of the mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase gene by insulin: the role of the forkhead transcription factor FKHRL1.
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Major histocompatibility complex (MHC) class I gene expression in single neurons of the central nervous system: differential regulation by interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha.
Neumann H et al.
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Conteúdo relacionado
Instructions
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I'm using this product (I6634) for my culture medium. Recently, I found my GLP-1 ELISA kit detects this insulin powder. I'm wondering if this insulin powder is purified from pancreatic extract using an antibody-based method, or simply a concentrated form.
1 answer-
Helpful?
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How is your cell culture grade insulin from bovine pancreas product is extracted and purified? Is the pancreas tissue homogenized prior to insulin protein extraction? Are any steps taken to remove contaminants? What is the estimated purity?
1 answer-
The manufacturing method is considered confidential. This product is not tested the purity. The potency is tested for each lot and reported on the Certificate of Analysis.
Please see the link below to access a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/i6634#product-documentationHelpful?
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To make a dilution of the stock prepared at 10 mg/ml in HEPES (i-e.10uM which is 34-fold than 2mg/ml) should I still use acidified water, or can i just dilute in PBS or water? This 10 uM dilution will be further dilute 1000 times for cell culture
1 answer-
A stock solution in HEPES at 10 mg/mL, such as that offered as product I0516, may be diluted with water, medium, or the aqueous buffer of choice. This would includes HEPES or PBS. Please see the link below to review the product information sheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/132/262/i6634pis.pdfHelpful?
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How can i make solution of insulin in HEPES buffer and how this solution is storage?
1 answer-
Preparation of Insulin solution: 10 mg/mL insulin in 25 mM HEPES. Recommended storage temperature 2 - 8 °C.
Helpful?
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How can I store the insulin solution? How long?
1 answer-
The USP recommends that solutions of insulin be stored at 2-8°C. It is not recommended to freeze solutions of insulin, as it can aggregate. Sterile solutions should be stable for at least 1 year at 2-8°C.
Helpful?
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What is the comparison between International Units (IU) and USP units for insulin?
1 answer-
The international unit (IU) of insulin is based on the International Standard Preparation of Bioassay (1958). The standard preparation is a mixture of 52% bovine and 48% porcine insulin, containing 24 units/mg. Reference: Martindale: The Extra Pharmacopoeia, 29th ed., p. 392. High purity bovine pancreatic insulin typically assays at approximately 27 USP units per mg. So the IU and USP unit are not identical, but they are approximately 1:1.
Helpful?
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What can be used to make a solution of insulin?
1 answer-
Insulin is soluble in dilute acids and alkalis. The solubility of insulin is tested at 2 mg/mL in water (acidified to pH 2 with hydrochloric acid). One can also use 1 mL of water acidified to pH 2 with 10 μL of glacial acetic acid to dissolve 2 mg of insulin. A 10 mg/mL solution of bovine insulin in 25 mM HEPES at pH 8.2 is offered as Product No. I0516.
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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What is the amino acid sequence for Product I6634, Insulin from bovine pancreas?
1 answer-
The sequence and structure of insulin can be found on the product information sheet (under Documents, above).
Helpful?
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How many units of insulin are present in my vial of Product I6634, Insulin from bovine pancreas?
1 answer-
Product No. I6634 has a specification of not less than 27 USP units/mg. The exact value for the product received can be found the on lot-specific Certificate of Analysis.
Helpful?
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