The specification for the product does not include units of potency.
However, the potency is typically about 28 -29 IU/mg.
I9278
Insulina
sterile-filtered, BioXtra, suitable for cell culture
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About This Item
Preço e disponibilidade não estão disponíveis no momento.
Produtos recomendados
fonte biológica
Saccharomyces cerevisiae
Nível de qualidade
esterilidade
sterile-filtered
linha de produto
BioXtra
Formulário
solution
concentração
9.5-11.5 mg/mL
técnica(s)
cell culture | mammalian: suitable
Impurezas
≤50 EU/mL endotoxin
pH
7.9-8.5
nº de adesão UniProt
temperatura de armazenamento
2-8°C
chave InChI
PBGKTOXHQIOBKM-FHFVDXKLSA-N
Informações sobre genes
human ... INS(3630)
Descrição geral
A insulina é um hormônio polipeptídico de duas cadeias produzido pelas células β das ilhotas pancreáticas. Seu peso molecular é de cerca de 5800 Da. As cadeias α e β são ligadas por duas pontes dissulfeto entre as cadeias. A cadeia α contém uma ponte dissulfeto dentro da cadeia.
Aplicação
A solução de insulina humana tem sido usada como suplemento no meio de Eagle modificado (DMEM) de Dulbecco para cultura de linhagem celular epitelial e linhagem celular endometrial humana, meio essencial mínimo Eagle para cultura de linhagem celular de adenocarcinoma de mama humano e meio 199 para cultivar a linhagem de células ovarianas normais.
Solução de insulina humana é recomendada para uso em aplicações de cultura celular em concentração de 0,5 a 1 ml/l por litro de meio.
A solução de insulina humana foi usada para teste de tolerância de insulina em camundongos e ratos.[1][2] Ela também foi usada para diferenciação adipogênica de culturas de crescimento derivadas de células-tronco embrionárias humanas (hESCs) e células estromais mesenquimais humanas (hMSCs).[3][4]
A solução de insulina humana foi usada para teste de tolerância de insulina em camundongos e ratos.[1][2] Ela também foi usada para diferenciação adipogênica de culturas de crescimento derivadas de células-tronco embrionárias humanas (hESCs) e células estromais mesenquimais humanas (hMSCs).[3][4]
Ações bioquímicas/fisiológicas
A insulina desempenha um papel em vários processos celulares, como promover o crescimento celular, regular o ciclo celular e facilitar a captação de glicose. É comumente usada como suplemento proteico em meios de cultura de células de mamíferos livres de soro para ajudar a promover o crescimento ideal e a manutenção de células de mamíferos.
Outras notas
InChI: 1S/C257H383N65O77S6/c1-29-131(23)205(313-193(339)104-259)252(393)317-204(130(21)22)248(389)288-159(75-82-200(349)350)217(358)282-156(71-78-189(263)335)221(362)308-183-116-403-404-117-184-243(384)305-178(111-324)240(381)294-162(88-123(7)8)225(366)295-168(95-140-53-61-146(329)62-54-140)228(369)283-154(69-76-187(261)333)218(359)290-161(87-122(5)6)223(364)285-158(74-81-199(347)348)220(361)302-174(101-190(264)336)235(376)298-170(97-142-57-65-148(331)66-58-142)231(372)309-182(242(383)304-176(255(396)397)103-192(266)338)115-402-401-114-181(214(355)273-107-194(340)278-153(72-79-197(343)344)216(357)281-151(51-42-84-271-257(267)268)212(353)272-108-195(341)279-166(93-138-46-36-32-37-47-138)227(368)297-167(94-139-48-38-33-39-49-139)230(371)299-171(98-143-59-67-149(332)68-60-143)238(379)320-208(135(27)327)254(395)322-85-43-52-186(322)246(387)286-152(50-40-41-83-258)222(363)321-209(136(28)328)256(398)399)311-250(391)203(129(19)20)316-236(377)164(90-125(11)12)292-229(370)169(96-141-55-63-147(330)64-56-141)296-224(365)160(86-121(3)4)289-210(351)133(25)277-215(356)157(73-80-198(345)346)287-247(388)202(128(17)18)315-237(378)165(91-126(13)14)293-233(374)173(100-145-106-270-120-276-145)301-239(380)177(110-323)280-196(342)109-274-213(354)180(113-400-405-118-185(310-244(183)385)245(386)319-207(134(26)326)253(394)306-179(112-325)241(382)318-206(132(24)30-2)251(392)312-184)307-226(367)163(89-124(9)10)291-232(373)172(99-144-105-269-119-275-144)300-219(360)155(70-77-188(262)334)284-234(375)175(102-191(265)337)303-249(390)201(127(15)16)314-211(352)150(260)92-137-44-34-31-35-45-137/h31-39,44-49,53-68,105-106,119-136,150-186,201-209,323-332H,29-30,40-43,50-52,69-104,107-118,258-260H2,1-28H3,(H2,261,333)(H2,262,334)(H2,263,335)(H2,264,336)(H2,265,337)(H2,266,338)(H,269,275)(H,270,276)(H,272,353)(H,273,355)(H,274,354)(H,277,356)(H,278,340)(H,279,341)(H,280,342)(H,281,357)(H,282,358)(H,283,369)(H,284,375)(H,285,364)(H,286,387)(H,287,388)(H,288,389)(H,289,351)(H,290,359)(H,291,373)(H,292,370)(H,293,374)(H,294,381)(H,295,366)(H,296,365)(H,297,368)(H,298,376)(H,299,371)(H,300,360)(H,301,380)(H,302,361)(H,303,390)(H,304,383)(H,305,384)(H,306,394)(H,307,367)(H,308,362)(H,309,372)(H,310,385)(H,311,391)(H,312,392)(H,313,339)(H,314,352)(H,315,378)(H,316,377)(H,317,393)(H,318,382)(H,319,386)(H,320,379)(H,321,363)(H,343,344)(H,345,346)(H,347,348)(H,349,350)(H,396,397)(H,398,399)(H4,267,268,271)/t131-,132-,133-,134+,135+,136+,150-,151-,152-,153-,154-,155-,156-,157-,158-,159-,160-,161-,162-,163-,164-,165-,166-,167-,168-,169-,170-,171-,172-,173-,174-,175-,176-,177-,178-,179-,180-,181-,182-,183-,184-,185-,186-,201-,202-,203-,204-,205-,206-,207-,208-,209-/m0/s1
forma física
estável a 2-8 °C
produto relacionado
Nº do produto
Descrição
Preços
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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What is the concentration of this solution in (Units/mL) i.e. if my stock is at a concentration of 11.1 mg/mL, what is that in Units/mL?
1 answer-
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What is the source of this insulin and is the solutuion expected to also contain proinsulin?
1 answer-
This product is a biosynthetic product, produced by recombinant expression in yeast (Saccharomyces cerevisiae), with a synthetic gene insert encoding human insulin precursor. The proinsulin content is not among the testing specifications for this product.
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How is this insulin produced? In E.Coli? Yeast?...?
1 answer-
This product is prepared from recombinant human insulin produced in Saccharomyces cerevisiae.
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Is this product suitable for insulin tolerance tests in mice
1 answer-
This item has not been specifically tested for suitability in insulin tolerance. However, the product has been used successfully in a number of published papers. Please see the link below to review one such article:
https://pmc.ncbi.nlm.nih.gov/articles/PMC2785638/Helpful?
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How long does the insulin remain potent for if stored in the fridge?
1 answer-
This product is assigned an expiration date of approximately 2 years from the date of Quality Release, when stored as directed at 2-8°C. The expiration date is lot specific and reported on the product label, as well as the Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/i9278#product-documentationHelpful?
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Is this insulin considered of fast or slow action?
1 answer-
This product is intended for research use only and is not a Therapeutic insulin. The research grade insulin products are not defined by the Therapeutic types. There are analytical standards available for each type. Please see the links below to review additional product options for research use:
Insulin Glargine - Slow acting Insulin
https://www.sigmaaldrich.com/search/insulin-glargine?focus=products&page=1&perpage=30&sort=relevance&term=insulin%20glargine&type=productAspart Insulin - Fast acting Insulin
https://www.sigmaaldrich.com/search/insulin-aspart?focus=products&page=1&perpage=30&sort=relevance&term=insulin%20aspart&type=productLispro Insulin - Fast acting Insulin
https://www.sigmaaldrich.com/search/insulin-lispro?focus=products&page=1&perpage=30&sort=relevance&term=insulin%20lispro&type=productHelpful?
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Is this human insulin suitable for use in mouse cell culture?
1 answer-
Yes, this product is suitable for mouse cell culture and has been cell culture tested on the L929 and 3T3 mouse cell lines. Please see the link below to review peer-reviewed articles that may be of interest:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6892261/#:~:text=This%20process%20requires%20DRAM%2D1,and%20permitting%20efficient%20mTORC1%20activation.Helpful?
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what is the morality of the product I9278, Insulin solution human?
1 answer-
The molarity of a 10 mg/mL solution of human insulin, with a molecular weight of 5807 daltons, is approximately 1.7 μmol/mL or 1.7 mM.
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What kind of solution is the insulin (Product number: I9278) dissolved in?
1 answer-
The insulin (cat# I9278) is dissolved in HEPES solution.
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Are there any contaminants (IgG, IgM example) in I9278?
1 answer-
Unfortunately, this product is not tested for impurities.
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