This is in HEPES. 10mg/mL concentration in 25 mM Hepes
I9278
Insulina
sterile-filtered, BioXtra, suitable for cell culture
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Sobre este item
Form:
solution
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Assistência Técnica
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Estamos aqui para ajudarGeneral description
A insulina é um hormônio polipeptídico de duas cadeias produzido pelas células β das ilhotas pancreáticas. Seu peso molecular é de cerca de 5800 Da. As cadeias α e β são ligadas por duas pontes dissulfeto entre as cadeias. A cadeia α contém uma ponte dissulfeto dentro da cadeia.
Application
A solução de insulina humana tem sido usada como suplemento no meio de Eagle modificado (DMEM) de Dulbecco para cultura de linhagem celular epitelial e linhagem celular endometrial humana, meio essencial mínimo Eagle para cultura de linhagem celular de adenocarcinoma de mama humano e meio 199 para cultivar a linhagem de células ovarianas normais.
Solução de insulina humana é recomendada para uso em aplicações de cultura celular em concentração de 0,5 a 1 ml/l por litro de meio.
A solução de insulina humana foi usada para teste de tolerância de insulina em camundongos e ratos.[1] Ela também foi usada para diferenciação adipogênica de culturas de crescimento derivadas de células-tronco embrionárias humanas (hESCs) e células estromais mesenquimais humanas (hMSCs).[2]
A solução de insulina humana foi usada para teste de tolerância de insulina em camundongos e ratos.[1] Ela também foi usada para diferenciação adipogênica de culturas de crescimento derivadas de células-tronco embrionárias humanas (hESCs) e células estromais mesenquimais humanas (hMSCs).[2]
Biochem/physiol Actions
A insulina desempenha um papel em vários processos celulares, como promover o crescimento celular, regular o ciclo celular e facilitar a captação de glicose. É comumente usada como suplemento proteico em meios de cultura de células de mamíferos livres de soro para ajudar a promover o crescimento ideal e a manutenção de células de mamíferos.
Physical form
estável a 2-8 °C
Other Notes
InChI: 1S/C257H383N65O77S6/c1-29-131(23)205(313-193(339)104-259)252(393)317-204(130(21)22)248(389)288-159(75-82-200(349)350)217(358)282-156(71-78-189(263)335)221(362)308-183-116-403-404-117-184-243(384)305-178(111-324)240(381)294-162(88-123(7)8)225(366)295-168(95-140-53-61-146(329)62-54-140)228(369)283-154(69-76-187(261)333)218(359)290-161(87-122(5)6)223(364)285-158(74-81-199(347)348)220(361)302-174(101-190(264)336)235(376)298-170(97-142-57-65-148(331)66-58-142)231(372)309-182(242(383)304-176(255(396)397)103-192(266)338)115-402-401-114-181(214(355)273-107-194(340)278-153(72-79-197(343)344)216(357)281-151(51-42-84-271-257(267)268)212(353)272-108-195(341)279-166(93-138-46-36-32-37-47-138)227(368)297-167(94-139-48-38-33-39-49-139)230(371)299-171(98-143-59-67-149(332)68-60-143)238(379)320-208(135(27)327)254(395)322-85-43-52-186(322)246(387)286-152(50-40-41-83-258)222(363)321-209(136(28)328)256(398)399)311-250(391)203(129(19)20)316-236(377)164(90-125(11)12)292-229(370)169(96-141-55-63-147(330)64-56-141)296-224(365)160(86-121(3)4)289-210(351)133(25)277-215(356)157(73-80-198(345)346)287-247(388)202(128(17)18)315-237(378)165(91-126(13)14)293-233(374)173(100-145-106-270-120-276-145)301-239(380)177(110-323)280-196(342)109-274-213(354)180(113-400-405-118-185(310-244(183)385)245(386)319-207(134(26)326)253(394)306-179(112-325)241(382)318-206(132(24)30-2)251(392)312-184)307-226(367)163(89-124(9)10)291-232(373)172(99-144-105-269-119-275-144)300-219(360)155(70-77-188(262)334)284-234(375)175(102-191(265)337)303-249(390)201(127(15)16)314-211(352)150(260)92-137-44-34-31-35-45-137/h31-39,44-49,53-68,105-106,119-136,150-186,201-209,323-332H,29-30,40-43,50-52,69-104,107-118,258-260H2,1-28H3,(H2,261,333)(H2,262,334)(H2,263,335)(H2,264,336)(H2,265,337)(H2,266,338)(H,269,275)(H,270,276)(H,272,353)(H,273,355)(H,274,354)(H,277,356)(H,278,340)(H,279,341)(H,280,342)(H,281,357)(H,282,358)(H,283,369)(H,284,375)(H,285,364)(H,286,387)(H,287,388)(H,288,389)(H,289,351)(H,290,359)(H,291,373)(H,292,370)(H,293,374)(H,294,381)(H,295,366)(H,296,365)(H,297,368)(H,298,376)(H,299,371)(H,300,360)(H,301,380)(H,302,361)(H,303,390)(H,304,383)(H,305,384)(H,306,394)(H,307,367)(H,308,362)(H,309,372)(H,310,385)(H,311,391)(H,312,392)(H,313,339)(H,314,352)(H,315,378)(H,316,377)(H,317,393)(H,318,382)(H,319,386)(H,320,379)(H,321,363)(H,343,344)(H,345,346)(H,347,348)(H,349,350)(H,396,397)(H,398,399)(H4,267,268,271)/t131-,132-,133-,134+,135+,136+,150-,151-,152-,153-,154-,155-,156-,157-,158-,159-,160-,161-,162-,163-,164-,165-,166-,167-,168-,169-,170-,171-,172-,173-,174-,175-,176-,177-,178-,179-,180-,181-,182-,183-,184-,185-,186-,201-,202-,203-,204-,205-,206-,207-,208-,209-/m0/s1
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Este Item | |||
|---|---|---|---|
| product line BioXtra | product line - | product line - | product line - |
| Quality Level 400 | Quality Level 200 | Quality Level - | Quality Level 300 |
| concentration 9.5-11.5 mg/mL | concentration >75% | concentration - | concentration - |
| form solution | form powder | form lyophilized (clear, colorless solution after reconstitution) | form - |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. -10 to -25°C |
Classe de armazenamento
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Número de item de comércio global
| SKU | GTIN |
|---|---|
| I9278-5ML | 04061838066626 |
| I9278-15ML | 04061838387431 |
| I9278-100ML | 04061838439963 |
| I9278-200ML | 04061838387448 |
| I9278-50ML | 04061838387455 |
| I9278-10ML | 04061838387424 |
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In which solution was insulin dissolved?
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What's the half-life of this insulin within the media at 37C?
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Unfortunately, the half-life of this insulin within the media at 37 °C has not been tested.
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For how long this can be stored @ RT?
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This item is stored refrigerated at 2-8°C. However, the product is shipped at ambient temperature. Shipping routes are configured to minimize transit time.
Refrigerated products that ship at ambient temperature are considered stable up to 2 weeks for transit time.Helpful?
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Can I aliquot the insulin and freeze it? Is it still functional after thawing?
1 answer-
This product is recommended to be stored at 2-8°C. For research applications, freezing insulin solution is a common practice. However, solutions available on the commercial market for therapeutic use are not frozen to maintain maximum activity. No stability studies have been performed on the shelf life of frozen insulin solutions.
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What is the concentration of this solution in (Units/mL) i.e. if my stock is at a concentration of 11.1 mg/mL, what is that in Units/mL?
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The specification for the product does not include units of potency.
However, the potency is typically about 28 -29 IU/mg.Helpful?
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What is the source of this insulin and is the solutuion expected to also contain proinsulin?
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This product is a biosynthetic product, produced by recombinant expression in yeast (Saccharomyces cerevisiae), with a synthetic gene insert encoding human insulin precursor. The proinsulin content is not among the testing specifications for this product.
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How is this insulin produced? In E.Coli? Yeast?...?
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This product is prepared from recombinant human insulin produced in Saccharomyces cerevisiae.
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Is this product suitable for insulin tolerance tests in mice
1 answer-
This item has not been specifically tested for suitability in insulin tolerance. However, the product has been used successfully in a number of published papers. Please see the link below to review one such article:
https://pmc.ncbi.nlm.nih.gov/articles/PMC2785638/Helpful?
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How long does the insulin remain potent for if stored in the fridge?
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This product is assigned an expiration date of approximately 2 years from the date of Quality Release, when stored as directed at 2-8°C. The expiration date is lot specific and reported on the product label, as well as the Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/i9278#product-documentationHelpful?
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Is this insulin considered of fast or slow action?
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This product is intended for research use only and is not a Therapeutic insulin. The research grade insulin products are not defined by the Therapeutic types. There are analytical standards available for each type. Please see the links below to review additional product options for research use:
Insulin Glargine - Slow acting Insulin
https://www.sigmaaldrich.com/search/insulin-glargine?focus=products&page=1&perpage=30&sort=relevance&term=insulin%20glargine&type=productAspart Insulin - Fast acting Insulin
https://www.sigmaaldrich.com/search/insulin-aspart?focus=products&page=1&perpage=30&sort=relevance&term=insulin%20aspart&type=productLispro Insulin - Fast acting Insulin
https://www.sigmaaldrich.com/search/insulin-lispro?focus=products&page=1&perpage=30&sort=relevance&term=insulin%20lispro&type=productHelpful?
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