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L2140
Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)
Isolectin B4 (BSI-B4), biotin conjugate, lyophilized powder
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Synonym(s):
Bandeirea simplicifolia agglutinin, BS-I
NACRES:
NA.32
Nível de qualidade
200
fonte biológica
Bandieraea simplicifolia
conjugado
biotin conjugate
forma
lyophilized powder
potência
<50 μg per mL agglutination activity (using human blood group B erythrocytes)
composição
Protein, ~95% E1%/280
extensão da rotulagem
3-6 mol biotin per mol protein
temperatura de armazenamento
−20°C
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Descrição geral
Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) has A and B subunits, which recognizes αGalNAc and αGal-end groups respectively. Lectins are extracted from the seeds of the legume shrub Griffonia simplicifolia, which are carbohydrate binding proteins.
Aplicação
Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) has been used for immunohistochemistry and immunofluorescence staining.
Embalagem
0.2, 1 mg in serum bottle
Ações bioquímicas/fisiológicas
Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) agglutinates human blood group A and B cells. It is specific for αGalNAc- and αGal. It acts as a reagent for the detection of this αGal epitope in biological materials. Lectin from Bandeiraea simplicifolia is used as a marker for xenoreactive antigen (αGal1−3Gal) and cancer. Lectin plays an important role in fertilization, embryogenesis, inflammation, metastasis and host-parasite recognition.
BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.
Outras notas
BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A4 has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.
forma física
Contains citrate buffer salts and calcium chloride
Nota de análise
Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.
Código de classe de armazenamento
13 - Non Combustible Solids
WGK
WGK 3
Ponto de fulgor (ºF)
Not applicable
Ponto de fulgor (ºC)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
Certificate of Analysis
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Certificate of Origin
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Product Information Sheet
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