Merck
All Photos(2)

L2140

Sigma-Aldrich

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)

Isolectin B4 (BSI-B4), biotin conjugate, lyophilized powder

Synonym(s):
Bandeirea simplicifolia agglutinin, BS-I
NACRES:
NA.32

Nível de qualidade

200

fonte biológica

Bandieraea simplicifolia

conjugado

biotin conjugate

forma

lyophilized powder

potência

<50 μg per mL agglutination activity (using human blood group B erythrocytes)

composição

Protein, ~95% E1%/280

extensão da rotulagem

3-6 mol biotin per mol protein

temperatura de armazenamento

−20°C

Looking for similar products? Visit Product Comparison Guide

Descrição geral

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) has A and B subunits, which recognizes αGalNAc and αGal-end groups respectively. Lectins are extracted from the seeds of the legume shrub Griffonia simplicifolia, which are carbohydrate binding proteins.

Aplicação

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) has been used for immunohistochemistry and immunofluorescence staining.

Embalagem

0.2, 1 mg in serum bottle

Ações bioquímicas/fisiológicas

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) agglutinates human blood group A and B cells. It is specific for αGalNAc- and αGal. It acts as a reagent for the detection of this αGal epitope in biological materials. Lectin from Bandeiraea simplicifolia is used as a marker for xenoreactive antigen (αGal1−3Gal) and cancer. Lectin plays an important role in fertilization, embryogenesis, inflammation, metastasis and host-parasite recognition.
BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Outras notas

BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A4 has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.

forma física

Contains citrate buffer salts and calcium chloride

Nota de análise

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.

Código de classe de armazenamento

13 - Non Combustible Solids

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Product Information Sheet

SDS

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service