L4402

Sigma-Aldrich

Lysostaphin from Staphylococcus staphylolyticus

BioUltra, ≥97% (SDS-PAGE), Protein 40-60 % by biuret, ≥2,000 units/mg protein

Sinônimo(s):
Glycyl-glycine Endopeptidase
Número CAS:
Número da licença da enzima:
Número MDL:
NACRES:
NA.54

linha de produto

BioUltra

Nível de qualidade

300

teor

≥97% (SDS-PAGE)

forma

lyophilized powder

specific activity

≥2,000 units/mg protein

peso molecular

25 kDa

composição

Protein, 40-60% biuret

Modo de ação

cell wall synthesis | interferes

espectro de atividade do antibiótico

Gram-positive bacteria

temperatura de armazenamento

−20°C

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Descrição geral

Chemical structure: peptide
Package size based on protein content

Embalagem

0.5, 2, 5 mg in glass bottle

Ações bioquímicas/fisiológicas

Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic.
pH Optimum for activity: ~7.5

Definição da unidade

One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37 °C in a 6.0 ml reaction mixture.

forma física

Contains potassium phosphate buffer salts and sodium chloride

Nota de preparo

Affinity purified

Pictogramas

Health hazard

Palavra indicadora

Danger

Frases de perigo

Declarações de precaução

hazcat

Resp. Sens. 1

storage_class_code

11 - Combustible Solids

WGK Alemanha

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificado de análise

Certificado de origem

Johnny Gutierrez et al.
Open forum infectious diseases, 6(4), ofz090-ofz090 (2019-04-27)
Staphylococcus aureus (SA) bacteremia often requires a long treatment duration with antibiotics to prevent relapse due to the ability of SA to establish reservoirs of infection in sites such as heart and bone. These metastatic sites of infection cannot be...
Shaw R Gargis et al.
Applied and environmental microbiology, 76(20), 6944-6946 (2010-08-24)
Resistance to lysostaphin, a staphylolytic glycylglycine endopeptidase, is due to a FemABX-like immunity protein that inserts serines in place of some glycines in peptidoglycan cross bridges. These modifications inhibit both binding of the recombinant cell wall targeting domain and catalysis...
Igor Belyansky et al.
The American surgeon, 77(8), 1025-1031 (2011-09-29)
Mesh and wound infections during hernia repair are predominantly caused by Staphylococcus aureus. Human acellular dermis (HAD) is known to lose its integrity in the face of large bacterial loads. The goal of this study was to determine if lysostaphin...
Yuliya Yurko et al.
Surgical innovation, 19(1), 20-26 (2011-07-12)
Naturally occurring antimicrobial peptides are possibly the "next frontier" in infection prevention. Binding them to mesh could reduce the rate of mesh infections. This study identifies an antimicrobial agent capable of significant antibacterial activity when bound to mesh. Lysozyme, human...
Mohammad Mehdi Feizabadi et al.
Journal of microbiological methods, 84(1), 144-146 (2010-11-17)
A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Staphylococcus aureus and enterococci to reduce the cost of using lysostaphin. This protocol reduces the expenses of PFGE typing of S. aureus and enterococci as...

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