Merck
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L7543

Sigma-Aldrich

Anti-LC3B antibody produced in rabbit

enhanced validation

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-MAP1A/MAP1B LC3 B, Anti-Microtubule-associated proteins 1A/B light chain 3B precursor, LC3B Antibody - Anti-LC3B antibody produced in rabbit, Anti-Autophagy-related protein LC3 B, Lc3 Antibody Sigma, Anti-MAP1 light chain 3-like protein 2, Anti-Autophagy-related ubiquitin-like modifier LC3 B, Anti-MAP1A/1B light chain 3 B, Lc3B Antibody
Número MDL:
NACRES:
NA.41

fonte biológica

rabbit

Nível de qualidade

200

conjugado

unconjugated

forma do anticorpo

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

forma

buffered aqueous solution

peso molecular

antigen 16-18 kDa

species reactivity

rat, human, mouse

validação aprimorada

functional assay
Learn more about Antibody Enhanced Validation

concentração

~1 mg/mL

technique(s)

indirect immunofluorescence: 5-10 μg/mL using human HeLa cells
western blot: 1-2 μg/mL using whole extracts of rat and mouse brain

nº de adesão UniProt

application(s)

research pathology

enviado em

dry ice

temperatura de armazenamento

−20°C

Gene Information

Descrição geral

Microtubule-associated protein 1 light chain 3 (MAPLC3/LC3) was first identified in rat as a protein that co-purifies with microtubule-associated protein 1A and 1B from rat brain. LC3 exists in cells in two forms. One is cytoplasmic, LC3-I (18 kDa) and the other, LC3-II (16 kDa) is associated with the autophagosome membrane. LC3-I is modified to a membrane-bound form, LC3-II (a LC3-phospholipid conjugate), by mammalian autophagy related protein 7 (Atg7) and Atg3, which are E1- and E2-like enzymes, respectively. Three human orthologs of the rat LC3, named MAP1LC3A/LC3A, MAP1LC3B/LC3B, and MAP1LC3C/ LC3C, were identified. The human proteins exhibit two forms representing the cytosolic (type I) and the membrane associated (type II) forms. The three human isoforms show different expression patterns in human tissues.
The gene MAP1LC3B (microtubule associated protein 1 light chain 3 β) is a homolog of ATG8 gene, an autophagy-related gene in yeast. The encoded protein is called pro-MAP1LC3B, a pro-form that is cleaved by ATG4 to produce a cytosolic form called MAP1LC3B-I.

Especificidade

By immunoblotting, the antibody recognizes human, rat, and mouse LC3B-I and LC3B-II (~18 kDa and ~16 kDa, respectively).

Imunogênio

synthetic peptide corresponding to amino acids 2-15 of human LC3B, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence differs by one amino acid in rat and mouse.

Aplicação

Anti-LC3B antibody produced in rabbit has been used in western blotting and immunohistochemistry.

Ações bioquímicas/fisiológicas

Microtubule-associated protein 1 light chain 3 (MAPLC3/LC3) expression level correlates with the extent of autophagosome formation. LC3B can be used as an autophagosomal marker.
MAP1LC3B-I (microtubule associated protein 1 light chain 3 β-I) conjugates with MAP1LC3B-II before being inserted into both inner and outer membranes of the growing autophagic vesicle during autophagy. The cellular distribution and processing of MAP1LC3B facilitates monitoring of autophagy. Cells that lack MAP1LC3B are found to be defective in the formation of autophagic vesicles, with altered phenotypes associated with the role of autophagy in energy homeostasis and removal of protein aggregates. It can serve as an autophagic marker.

forma física

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de classe de armazenamento

10 - Combustible liquids

WGK

WGK 1

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificate of Analysis

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Certificate of Origin

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SDS

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