Lysostaphin from Staphylococcus simulans

recombinant, expressed in E. coli, lyophilized powder

Glycyl-glycine Endopeptidase
Número CAS:
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Nível de qualidade



expressed in E. coli


≥90% (HPLC)


lyophilized powder

specific activity

≥3000 unit/mg solid

enviado em

dry ice

temperatura de armazenamento


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Descrição geral

Lysostaphin, a zinc metalloenzyme, is mainly secreted as a proenzyme. It acts as an anti-staphylococcal agent and is highly stable at pH 4 and 5°C.


Lysostaphin from Staphylococcus simulans has been used in a study to assess molecular cloning, sequencing, and expression of lytM, a unique autolytic gene of Staphylococcus aureus. It has also been used in a study to investigate the sequence analysis of a Staphylococcus aureus gene encoding a peptidoglycan hydrolase activity.


5 mg in glass bottle

Ações bioquímicas/fisiológicas

Lysostaphin possesses a lytic action against Staphylococcus aureus, which is used to treat antibiotic-resistant staphylococcal infections. It has the properties of enzymes, such as glycylglycine endopeptidase, endo-β-N-acetyl glucosamidase and N-acetyl muramyl-L-alanine amidase. Lysostaphin is also used as a preservative in food industry and in clinical labs for rapid screening.
A protein complex with highly specific lytic activity against Staphylococcus species, including Staphylococcus aureus.
The antimicrobial activity of lysostaphin, a metalloendopeptidase expressed by Staphylococcus simulans, is enhanced by binding to the C′ terminus of the membrane-associated Trap protein of Staphylococcus aureus.


Health hazard

Palavra indicadora


Frases de perigo

Declarações de precaução


Resp. Sens. 1


11 - Combustible Solids

WGK Alemanha


Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificado de análise

Certificado de origem

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

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  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How should a stock solution of the Lysostaphin from Staphylococcus simulans enzyme, product L9043, be prepared?

    A stock solution of this recombinant, Lysostaphin from Staphylococcus simulans, may be prepared by reconstituting it at 10 mg/ml in 20 mM sodium acetate buffer, pH 4.5 and storing it aseptically at 4°C. in a polypropylene container. 

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L Ramadurai et al.
Journal of bacteriology, 179(11), 3625-3631 (1997-06-01)
A gene encoding an autolytic activity was identified in an autolysis-deficient mutant (Lyt-) of Staphylococcus aureus which produces only a single band in autolytic-activity gels (N. Mani, P. Tobin, and R. K. Jayaswal, J. Bacteriol. 175:1493-1499, 1993). An open reading...
C-terminus of TRAP in Staphylococcus can enhance the activity of lysozyme and lysostaphin.
Yang, G.
Acta Biochimica et Biophysica Hungarica, 40, 452-458 (2008)
H P DeHart et al.
Applied and environmental microbiology, 61(4), 1475-1479 (1995-04-01)
Staphylococcus simulans biovar staphylolyticus produces an extracellular glycylglycine endopeptidase (lysostaphin) that lyses other staphylococci by hydrolyzing the cross bridges in their cell wall peptidoglycans. The genes for endopeptidase (end) and endopeptidase resistance (epr) reside on plasmid pACK1. An 8.4-kb fragment...
X Wang et al.
Gene, 102(1), 105-109 (1991-06-15)
The nucleotide (nt) sequence of a 2.0-kb NheI-XbaI DNA fragment containing a peptidoglycan hydrolase-encoding gene, lytA, tentatively identified as encoding an N-acetylmuramyl-L-alanine amidase, from Staphylococcus aureus, was determined. The nt sequencing revealed an open reading frame (ORF) of 1443 bp...
Grégory Francius et al.
Journal of bacteriology, 190(24), 7904-7909 (2008-10-07)
The advent of Staphylococcus aureus strains that are resistant to virtually all antibiotics has increased the need for new antistaphylococcal agents. An example of such a potential therapeutic is lysostaphin, an enzyme that specifically cleaves the S. aureus peptidoglycan, thereby...

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