Merck
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M1028

Sigma-Aldrich

Magnesium chloride solution

for molecular biology, 1.00 M±0.01 M

Todas as fotos(2)
Fórmula linear:
MgCl2
Número CAS:
7786-30-3
Peso molecular:
95.21
Número MDL:
MFCD00011106
ID de substância PubChem:
24896582
NACRES:
NA.52

Nível de qualidade

300

grau

for molecular biology

esterilidade

non-sterile; 0.2 μm filtered

forma

liquid

concentração

1.00 M±0.01 M

traços de cátion

heavy metals (as Pb): ≤5 ppm

atividade externa

DNase, RNase, NICKase and protease, none detected

SMILES string

Cl[Mg]Cl

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

InChI key

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

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Descrição geral

Magnesium is a typical ionic halide, and is very soluble in water. Magnesium has a variety of biological roles in enzymology, cell membrane/wall structure, muscle cell physiology, and nucleic acid structure. Magnesium is an essential co-factor in many enzymes, including DNAse, some restriction enzymes, and Ribonuclease H.

Magnesium chloride is widely used to supply the magnesium ion in various molecular biology applications, including PCR reactions.

Aplicação

Magnesium chloride is widely used to supply the magnesium ion in various molecular biology applications, including PCR reactions and buffers. It was used to prepare buffer to lyse cells for immunoprecipitation and to prepare serum-free medium for electrophysiological studies of rat brain slice cultures.

Componentes

Magnesium chloride solution contains 1M magnesium chloride in 18 megaohm water.

Código de classe de armazenamento

12 - Non Combustible Liquids

WGK

WGK 1

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves

Certificado de análise

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Certificado de origem

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Product Information Sheet

Product Information Sheet - M1028

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Artigos

Multiplex qPCR with JumpStart™ Taq ReadyMix™ for Quantitative PCR

Method outlines use of a hot start Taq for multiplex qPCR and provides guidance on how to optimize dNTPs, primer, probes and MgCL2 concentrations. By optimizing these parameters, the user can improve assay sensitivity and linear range of detection.

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