M2444

Sigma-Aldrich

Monoclonal Anti-MDC1 antibody produced in mouse

clone MDC1-50, purified immunoglobulin, buffered aqueous solution

Sinônimo(s):
Anti-Mediator of DNA Damage Checkpoint Protein 1
Número MDL:
NACRES:
NA.41

Nível de qualidade

200

fonte biológica

mouse

forma do anticorpo

purified immunoglobulin

antibody product type

primary antibodies

clone

MDC1-50, monoclonal

forma

buffered aqueous solution

peso molecular

antigen ~250 kDa (2-3 bands)

species reactivity

monkey, human

embalagem

antibody small pack of 25 μL

aplicação(ões)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1-2 μg/mL using total cell extract of G361 cells

Isotipo

IgG2a

conjugado

unconjugated

nº de adesão UniProt

enviado em

dry ice

temperatura de armazenamento

−20°C

Gene Information

human ... MDC1(9656)

Categorias relacionadas

Descrição geral

Monoclonal Anti-MDC1 (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a recombinant human MDC1 fragment. Mediator of DNA damage checkpoint protein 1 (MDC1) contains 2089 amino-acid residues with a predicted molecular weight of 226.4 kDa. The protein contains an FHA (forkhead-associated) domain at its amino terminus and two BRCT (BRCA1 carboxyl terminal) domains at its carboxy terminus.

Especificidade

Mouse monoclonal clone MDC1-50 anti-MDC1 antibody recognizes human and monkey MDC1.

Imunogênio

recombinant human MDC1 fragement (amino acids 2-200).

Aplicação

Monoclonal Anti-MDC1 antibody produced in mouse has been used in:
  • enzyme-linked immunosorbent assay (ELISA
  • immunofluorescence
  • western blotting
  • immunoprecipitation
  • immunocytochemistry

Mouse monoclonal clone MDC1-50 anti-MDC1 antibody is used to tag mediator of DNA damage checkpoint protein 1 for detection and quantitation by Western blotting and immunohistochemical (IHC) techniques such as ELISA, immunoblotting (approx. 250 kDa, 2-3 bands), immunoprecipitation and immunocytochemistry. It is used as a probe to determine the roles of mediator of DNA damage checkpoint protein 1 in DNA repair and genomic stability.

Ações bioquímicas/fisiológicas

Genomic instability caused by the disruption of mechanisms that regulate cell-cycle checkpoints, DNA repair and apoptosis may lead to the development of cancer. ATM and ATR protein kinases are mediated to DNA damage sites by molecular adapters or mediators proteins such as Histone H2AX, Claspin and BRCTmotif containing molecules such as 53BP1, BRCA1, and MDC1 (mediator of DNA damage checkpoint protein 1). MDC1 interacts with the MRE11 complex (containing MRE11, RAD50 and NBS1 proteins). The MRE11 complex is involved in the detection repair and signaling of DNA damage. Upon ionizing radiation MDC1 is hyperphosphorylated by ATM and localizes to nuclear foci together with the MRE11 complex, phosphorylated H2AX and 53BP1. A radio resistant DNA synthesis (RDS) phenotype in cells is formed by down regulation of MDC1 protein expression by siRNA.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

10 - Combustible liquids

WGK Alemanha

WGK 2

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Certificado de análise

Certificado de origem

Helen C Turner et al.
Radiation and environmental biophysics, 53(2), 265-272 (2014-01-31)
At the Center for High-Throughput Minimally Invasive Radiation Biodosimetry, we have developed a rapid automated biodosimetry tool (RABiT); this is a completely automated, ultra-high-throughput robotically based biodosimetry workstation designed for use following a large-scale radiological event, to perform radiation biodosimetry...
J M Bradbury et al.
Biochemical Society transactions, 31(Pt 1), 40-44 (2003-01-28)
To maintain genomic stability, despite constant exposure to agents that damage DNA, eukaryotic cells have developed elaborate and highly conserved pathways of DNA damage sensing, signalling and repair. In this review, we concentrate mainly on what we know about DNA...
A dual interaction between the DNA damage response protein MDC1 and the RAG1 subunit of the V (D) J recombinase
Coster G, et al.
The Journal of biological chemistry, 287(43), 36488-36498 (2012)
DICER, DROSHA and DNA damage response RNAs are necessary for the secondary recruitment of DNA damage response factors
Francia S, et al.
Journal of Cell Science, 129(7), 1468-1476 (2016)
Qiang Chen et al.
Nucleic acids research, 44(19), 9266-9278 (2016-11-02)
O-linked N-acetylglucosamine linkage (O-GlcNAcylation) to serine or threonine residues regulates numerous biological processes; however, its role in DNA damage response remains elusive. Here, we found that O-GlcNAcylation is induced by DNA damage response. O-GlcNAc transferase (OGT), the solo enzyme for...

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