Merck
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M6444

Sigma-Aldrich

Anti-Mitofusin 2 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-CMT2A, Anti-CMT2A2, Anti-CPRP1, Anti-KIAA0214, Anti-MARF, Anti-Mfn2
Número MDL:
NACRES:
NA.41

fonte biológica

rabbit

Nível de qualidade

200

conjugado

unconjugated

forma do anticorpo

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

forma

buffered aqueous solution

peso molecular

antigen ~86 kDa

species reactivity

human, mouse, rat

concentração

~1 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using HeLa human epitheloid carcinoma cell lysate
western blot (chemiluminescent): 0.5-1 μg/mL using extract of mouse and rat brain mitochondria

nº de adesão UniProt

enviado em

dry ice

temperatura de armazenamento

−20°C

Gene Information

human ... MFN2(9927)
mouse ... Mfn2(170731)
rat ... Mfn2(64476)

Descrição geral

Anti-Mitofusin 2 is produced in rabbit using as immunogen a synthetic peptide corresponding to human mitofusin 2 with an N-terminal added cysteine, conjugated to KLH. Mitofusins (Mfn1 and Mfn2) form homotypic and heterotypic complexes for fusion in mitochondria.[1]
Mitofusin-2 (MFN2) is an outer mitochondrial membrane protein.[2]

Imunogênio

synthetic peptide corresponding to amino acid residues 557-576 of human mitofusin 2 with an N-terminal added cysteine, conjugated to KLH. The corresponding sequence differs by one and two amino acids in rat and mouse mitofusin 2, respectively.

Aplicação

Anti-Mitofusin 2 antibody has been used in immunoblotting.[3] It may also be used in immunoprecipitation.
Anti-Mitofusin 2 antibody produced in rabbit has been used for Western blotting.[4]
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Ações bioquímicas/fisiológicas

MFN2 is essential for the maintenance of mitochondrial network and controls mitochondrial metabolism.[1] Mfn2-dependent regulatory mechanism is disturbed in obesity by reduced Mfn2 expression. Mutations in MFN2 cause Charcot Marie-Tooth neuropathy type 2A, a neurological disorder that results from degeneration of axons in peripheral nerves.
Mitofusin-2 (MFN2) plays an important role as a mitochondrial fusion protein and participates in many cell processes. It is mainly involved in regulating metabolic processes in the mitochondria. MFN2 prevents the formation of reactive oxygen species and controls endoplasmic reticulum stress. Mutations in the gene encoding this protein have been linked to diabetes and obesity.[2][5]

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de classe de armazenamento

10 - Combustible liquids

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

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Certificate of Origin

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