M7027

Sigma-Aldrich

Methyl cellulose

viscosity: 15 cP, BioReagent, suitable for cell culture

Número CAS:
Número MDL:
ID de substância PubChem:
NACRES:
NA.75

Nível de qualidade

200

fonte biológica

synthetic (organic)

linha de produto

BioReagent

forma

powder

aplicação(ões)

cell culture | mammalian: suitable

viscosidade

12-18 cP, 2 % in H2O(20 °C)

solubilidade

water: 50 mg/mL, clear to hazy

SMILES string

[*]OC[C@H]1O[C@@H](O[C@@H]2[C@@H](CO[*])O[C@@H](O[*])[C@H](O[*])[C@H]2O[*])[C@H](O[*])[C@@H](O[*])[C@@H]1O[*]

InChI key

YLGXILFCIXHCMC-JHGZEJCSSA-N

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Aplicação

Methyl cellulose is used to create semi-solid matrices, as a cell culture gel, for culture of colony forming cells such as CFU-G; CFU-GM; cells and other cells used in toxicity assays. Low viscosity, not for plaquing assays or cloning.

Embalagem

100, 250 g in poly bottle

storage_class_code

13 - Non Combustible Solids

WGK Alemanha

WGK 1

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificado de análise

Certificado de origem

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How can I make a solution of Methyl cellulose, Product M7027?

    There are two methods for solubilizing methylcellulose. Please see the product insert for Product No. M0387 for the protocols.

  4. Can I use this Methyl cellulose, Product M7027, for cloning or plaque assays?

    Probably not. Product No. M7027 has a very low viscosity (viscosity 15 cP, 2% solution in water) and is used for thickening culture media.  Product No. M0387 (viscosity 1,500 cP, 2% solution in water) is recommended for plaque assays, and Product No. M0512 (viscosity 4,000 cP, 2% solution in water) is recommended for cloning or colony growth assays.

  5. How do I sterilize a solution of Methyl cellulose, Product M7027?

    A 0.5-1% solution of methylcellulose can be autoclaved using standard conditions (121 °C, 15 psi, 15-20 minutes). Higher concentrations may lead to chunks of methylcellulose falling out of solution.

  6. What concentration of Methyl cellulose, Product M7027, is used for cell culture?

    Product No. M7027 is used at approximately 50 g/L in media to enhance viscosity of culture media.

  7. Is Methyl cellulose, Product M7027, the same as Methocel?

    Methocel is a brand name and is a synonym for methylcellulose.

  8. How do I store a stock solution of Methyl cellulose, Product M7027?

    A sterile solution of methylcellulose can be stored at room temperature for up to 1 year.

  9. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  10. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

Large variability in the motility of spiroplasmas in media of different viscosities
Boudet JF, et al.
Scientific Reports, 8(1), 17138-17138 (2018)
Dissociated effect and Chemosensitive enhancement of tumor spheroids influenced by an electric field in a microdevice
Huang Chun-Hao, et al.
Biomedical Microdevices, 20(3), 70-70 (2018)
Chandler Warr et al.
ACS applied bio materials, 3(4), 2239-2244 (2020-05-30)
We report a non-cytotoxic resin compatible with and designed for use in custom high-resolution 3D printers that follow the design approach described in Gong et al., Lab Chip 17, 2899 (2017). The non-cytotoxic resin is based on a poly(ethylene glycol)...
High throughput and automatic colony formation assay based on impedance measurement technique
Lei KF, et al.
Analytical and Bioanalytical Chemistry, 409(12), 3271-3277 (2017)
Barley beta-glucan accelerates wound healing by favoring migration versus proliferation of human dermal fibroblasts
Fuste NP, et al.
Carbohydrate Polymers, 389-398 (2019)
Protocolos
Step-by-step hematopoietic stem cell culture protocols for isolation, expansion and differentiation of CD34+ hematopoietic progenitor cells including CFU assays. It can be cultured under defined conditions designed either to promote self-renewal and increase the number of primitive cells.
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