Merck
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N0513

Sigma-Aldrich

Nerve Growth Factor-7S from murine submaxillary gland

NGF-7S, lyophilized powder, suitable for cell culture

Synonym(s):
NGF-7S
CAS Number:
Número EC:
Número MDL:
NACRES:
NA.77

fonte biológica

mouse

Nível de qualidade

200

forma

lyophilized powder

potência

2-250 ng/mL ED50/EC50

qualidade

endotoxin tested

peso molecular

protein 130 kDa

embalagem

pkg of 0.1 mg

technique(s)

cell culture | mammalian: suitable

Impurezas

≤10

cor

white

nº de adesão UniProt

temperatura de armazenamento

−20°C

Gene Information

mouse ... Ngf(18049)

Descrição geral

Nerve growth factor (NGF) is a dimer of 118-residue polypeptide chains linked non-covalently. Both these polypeptides contain three intramolecular disulphide bridges. It forms the prototype of the neurotrophin growth factor family. In male mouse submandibular salivary gland, NGF is present in high amounts, and is present as a high molecular weight complex called nerve growth factor-7S (NGF-7S). NGF-7S is an α2β2γ2 complex, where β2-NGF is the active neurotrophin, and is linked to the dimers of the glandular kallikrein serine proteinases members, α-NGF and γ-NGF.

Aplicação

Nerve Growth Factor-7S (NGF-7S) from murine submaxillary gland has been used for the treatment of following cells to induce neuronal differentiation-
  • PC12
  • Dorsal root ganglia (DRG) obtained from chick embryos of either sex (Gallus gallus)

Ações bioquímicas/fisiológicas

Nerve growth factor (NGF) is a neurotrophin, which facilitates the differentiation and survival of specific subtypes of neurons of central nervous system and peripheral nervous system. However, nerve growth factor-7S (NGF-7S) lacks any neurotrophic activity as most part of β-NGF subunit is buried within the NGF-7S complex.

forma física

Lyophilized from 0.2 μm filtered solution in 25 mM sodium phosphate, pH 7.0.

Nota de preparo

Nerve growth factor-7S (NGF-7S) is isolated from male mouse submaxillary glands using a modification of the method of Varon, et al.

Nota de análise

The biological activity is measured using a 3-day MTT assay.

Código de classe de armazenamento

13 - Non Combustible Solids

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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